BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_K22 (970 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 35 0.020 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 0.023 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 33 0.080 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 32 0.14 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.24 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 31 0.32 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.56 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.75 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.7 SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 27 4.0 SPAC2F7.02c |||phosphoprotein phosphatase|Schizosaccharomyces po... 27 4.0 SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 27 5.3 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 34.7 bits (76), Expect = 0.020 Identities = 25/111 (22%), Positives = 32/111 (28%), Gaps = 3/111 (2%) Frame = +1 Query: 643 PXAPRXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHPPXRXXRLXHXXXRXXXXXXXX 822 P P+ PP P PP P + P+ PP + Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV 1161 Query: 823 XXXXXXXXPHPXPTA---PXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P P++ P P+ PP PP P G PP P Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212 Score = 33.1 bits (72), Expect = 0.060 Identities = 23/108 (21%), Positives = 29/108 (26%), Gaps = 3/108 (2%) Frame = +1 Query: 643 PXAPRXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHPPXRXXRLXH---XXXRXXXXX 813 P P+ PP P+ PP P + P+ PP + Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180 Query: 814 XXXXXXXXXXXPHPXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPP 957 P P P P+ PP PP P G PP Sbjct: 1181 VPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228 Score = 27.5 bits (58), Expect = 3.0 Identities = 23/105 (21%), Positives = 25/105 (23%) Frame = +1 Query: 643 PXAPRXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHPPXRXXRLXHXXXRXXXXXXXX 822 P P+ PP PL P + PT PP Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSS---SGAPSAPPPVPA 1068 Query: 823 XXXXXXXXPHPXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPP 957 P P P P PP P PP P PP Sbjct: 1069 PSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPP 1113 Score = 25.8 bits (54), Expect = 9.2 Identities = 13/40 (32%), Positives = 13/40 (32%) Frame = +1 Query: 847 PHPXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P AP P PP PP P G P P Sbjct: 1025 PLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPP 1064 Score = 25.8 bits (54), Expect = 9.2 Identities = 12/39 (30%), Positives = 13/39 (33%) Frame = +3 Query: 846 PXPXXXXPXPXLXXXPPPPPXXXSXTPXPXPPPWXPXXP 962 P P P P PP P + P P P P P Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVP 1230 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.1 bits (57), Expect = 4.0 Identities = 13/34 (38%), Positives = 14/34 (41%), Gaps = 1/34 (2%) Frame = +1 Query: 859 PTAPXPRXPXXPPPPXXXPPKP-PXPXXXRGXPP 957 PT+ PR PP P PP P P PP Sbjct: 140 PTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPP 173 Score = 26.6 bits (56), Expect(2) = 0.023 Identities = 14/44 (31%), Positives = 16/44 (36%), Gaps = 1/44 (2%) Frame = +1 Query: 634 PPXPXAP-RXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHPP 762 PP P + R PP P PP P P P+ PP Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPP 173 Score = 26.6 bits (56), Expect(2) = 0.023 Identities = 11/27 (40%), Positives = 11/27 (40%) Frame = +1 Query: 847 PHPXPTAPXPRXPXXPPPPXXXPPKPP 927 P P AP P P P PP PP Sbjct: 173 PPAQPAAPVKSPPSAPSLPSAVPPMPP 199 Score = 25.8 bits (54), Expect = 9.2 Identities = 10/24 (41%), Positives = 11/24 (45%) Frame = +1 Query: 853 PXPTAPXPRXPXXPPPPXXXPPKP 924 P P+ P P PP P PP P Sbjct: 145 PRPSIPPPSPASAPPIPSKAPPIP 168 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 32.7 bits (71), Expect = 0.080 Identities = 13/33 (39%), Positives = 15/33 (45%) Frame = +3 Query: 846 PXPXXXXPXPXLXXXPPPPPXXXSXTPXPXPPP 944 P P P P + PPPPP S +P P P Sbjct: 729 PAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAP 761 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 31.9 bits (69), Expect = 0.14 Identities = 13/33 (39%), Positives = 13/33 (39%) Frame = +1 Query: 868 PXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P PPPP PP P P G P P Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732 Score = 29.5 bits (63), Expect = 0.75 Identities = 13/38 (34%), Positives = 13/38 (34%) Frame = +1 Query: 853 PXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P AP PPPP P PP PP P Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAP 1731 Score = 28.7 bits (61), Expect = 1.3 Identities = 12/34 (35%), Positives = 12/34 (35%) Frame = +1 Query: 853 PXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXP 954 P P P P PP P P PP P P Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741 Score = 27.5 bits (58), Expect = 3.0 Identities = 12/36 (33%), Positives = 13/36 (36%) Frame = +1 Query: 859 PTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P + P P PP P PP P PP P Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAP 1721 Score = 27.1 bits (57), Expect = 4.0 Identities = 15/38 (39%), Positives = 15/38 (39%) Frame = +3 Query: 852 PXXXXPXPXLXXXPPPPPXXXSXTPXPXPPPWXPXXPP 965 P P PPPPP S P P PP P PP Sbjct: 1694 PQSAAPPQMSAPTPPPPP--MSVPPPPSAPP-MPAGPP 1728 Score = 26.6 bits (56), Expect = 5.3 Identities = 12/29 (41%), Positives = 12/29 (41%) Frame = +1 Query: 847 PHPXPTAPXPRXPXXPPPPXXXPPKPPXP 933 P P P P P P P PP PP P Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPP-PPLP 1736 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 31.1 bits (67), Expect = 0.24 Identities = 13/33 (39%), Positives = 13/33 (39%) Frame = +3 Query: 846 PXPXXXXPXPXLXXXPPPPPXXXSXTPXPXPPP 944 P P P P P PPP P P PPP Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767 Score = 30.7 bits (66), Expect = 0.32 Identities = 13/28 (46%), Positives = 13/28 (46%) Frame = +3 Query: 879 LXXXPPPPPXXXSXTPXPXPPPWXPXXP 962 L PPPPP TP P P P P P Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAP 756 Score = 30.7 bits (66), Expect = 0.32 Identities = 16/40 (40%), Positives = 16/40 (40%) Frame = +1 Query: 847 PHPXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P P AP P P P PP PP G PP P Sbjct: 742 PTPAP-APIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 Score = 30.3 bits (65), Expect = 0.43 Identities = 12/29 (41%), Positives = 13/29 (44%) Frame = +3 Query: 867 PXPXLXXXPPPPPXXXSXTPXPXPPPWXP 953 P P + PPPPP P PPP P Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 29.9 bits (64), Expect = 0.56 Identities = 14/40 (35%), Positives = 15/40 (37%) Frame = +3 Query: 846 PXPXXXXPXPXLXXXPPPPPXXXSXTPXPXPPPWXPXXPP 965 P P P + PPPPP PPP P PP Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP--PPPPP 783 Score = 29.1 bits (62), Expect = 0.98 Identities = 15/42 (35%), Positives = 15/42 (35%) Frame = +1 Query: 634 PPXPXAPRXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHP 759 PP P P GPP P PP PP S P P Sbjct: 762 PPPPPPPGVAGAGPPPP--PPPPPAVSAGGSRYYAPAPQAEP 801 Score = 26.2 bits (55), Expect = 6.9 Identities = 12/36 (33%), Positives = 12/36 (33%) Frame = +1 Query: 859 PTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P P P P P PP P PP P Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767 Score = 26.2 bits (55), Expect = 6.9 Identities = 14/36 (38%), Positives = 14/36 (38%), Gaps = 6/36 (16%) Frame = +1 Query: 859 PTAPXPRXPXXPPPPXXX------PPKPPXPXXXRG 948 P AP P PPPP PP PP P G Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAG 788 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 30.7 bits (66), Expect = 0.32 Identities = 19/54 (35%), Positives = 20/54 (37%), Gaps = 2/54 (3%) Frame = -1 Query: 784 GXTGXXGRVGGXXG--RXXGXRKXXGXXGVXXGGXAGRGGXKXXXXGPRGGGGV 629 G G G GG G R G G G G GRGG G +GG V Sbjct: 20 GFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGGAKV 73 Score = 26.6 bits (56), Expect = 5.3 Identities = 14/40 (35%), Positives = 14/40 (35%) Frame = -1 Query: 964 GGXXGXHGGGXGXGVXEXXXGGGGGFXXSXGXGXXXXGXG 845 GG G GG G GGG G G G G G Sbjct: 9 GGRGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRG 48 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 29.9 bits (64), Expect = 0.56 Identities = 15/39 (38%), Positives = 15/39 (38%), Gaps = 2/39 (5%) Frame = +1 Query: 847 PHPXPTAPX--PRXPXXPPPPXXXPPKPPXPXXXRGXPP 957 P P AP P P PP P P PP P PP Sbjct: 440 PMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478 Score = 29.1 bits (62), Expect = 0.98 Identities = 33/127 (25%), Positives = 36/127 (28%), Gaps = 7/127 (5%) Frame = +1 Query: 607 GRFSLXXXLPPXPXAPRXXXXG--PPXPLXPPXPPXCXPXXSGXRXXGPTXHPPXRXXRL 780 G S LPP P PR G P P PP P + P PP R Sbjct: 328 GNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQP---PPLSSSRA 384 Query: 781 XHXXXRXXXXXXXXXXXXXXXXPHPXPTA--PXPRXPXXPP--PPXXXPPKPP-XPXXXR 945 + T+ P P P PP PP P PP P Sbjct: 385 VSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAP 444 Query: 946 GXPPXXP 966 PP P Sbjct: 445 AAPPLPP 451 Score = 27.9 bits (59), Expect = 2.3 Identities = 14/40 (35%), Positives = 15/40 (37%) Frame = +1 Query: 847 PHPXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPPXXP 966 P P+ P P PP P P PP P PP P Sbjct: 433 PSAPPSLPMGA-PAAPPLPPSAPIAPPLPAGMPAAPPLPP 471 Score = 26.6 bits (56), Expect = 5.3 Identities = 28/126 (22%), Positives = 32/126 (25%), Gaps = 3/126 (2%) Frame = +1 Query: 589 VPGXGXGRFSLXXXLPPXPXAPRXXXXGPPXPLXPPXPPXCXPXXSGXRXXGPTXHPPXR 768 +P G S PP P AP PP PP + PP Sbjct: 256 IPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPP 315 Query: 769 XXRLXHXXXRXXXXXXXXXXXXXXXXPHPXPTAPXPRXPXXPPPPXXXPPKPP---XPXX 939 + R P P P + PP PP PP P Sbjct: 316 PPSRRN---RGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPST 372 Query: 940 XRGXPP 957 R PP Sbjct: 373 GRQPPP 378 Score = 26.2 bits (55), Expect = 6.9 Identities = 10/25 (40%), Positives = 11/25 (44%) Frame = +3 Query: 891 PPPPPXXXSXTPXPXPPPWXPXXPP 965 PPPPP + P PP PP Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPP 363 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 29.5 bits (63), Expect = 0.75 Identities = 31/98 (31%), Positives = 31/98 (31%) Frame = -1 Query: 964 GGXXGXHGGGXGXGVXEXXXGGGGGFXXSXGXGXXXXGXGXXXXXXXXXXXXXGXAXXFX 785 GG G GG G GG GGF G G G G G F Sbjct: 188 GGFGGFGGGSGG---PPPGPGGFGGFGGFGGEGHHHGGHG----------GFGGGPGGFE 234 Query: 784 GXTGXXGRVGGXXGRXXGXRKXXGXXGVXXGGXAGRGG 671 G G G GG G G G G GG G GG Sbjct: 235 GGPGGFG--GGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 28.3 bits (60), Expect = 1.7 Identities = 11/27 (40%), Positives = 11/27 (40%) Frame = +1 Query: 868 PXPRXPXXPPPPXXXPPKPPXPXXXRG 948 P P PPPP PP P P G Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPG 31 Score = 27.1 bits (57), Expect = 4.0 Identities = 10/21 (47%), Positives = 10/21 (47%) Frame = +3 Query: 891 PPPPPXXXSXTPXPXPPPWXP 953 PPPPP P PPP P Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29 Score = 26.2 bits (55), Expect = 6.9 Identities = 10/21 (47%), Positives = 10/21 (47%) Frame = +3 Query: 891 PPPPPXXXSXTPXPXPPPWXP 953 PPPPP P PPP P Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 27.1 bits (57), Expect = 4.0 Identities = 13/35 (37%), Positives = 13/35 (37%) Frame = +1 Query: 853 PXPTAPXPRXPXXPPPPXXXPPKPPXPXXXRGXPP 957 P P P P P PP P P P G PP Sbjct: 108 PLPNEPVPEEPLPGEPPLPDEPVPEEPLP--GEPP 140 >SPAC2F7.02c |||phosphoprotein phosphatase|Schizosaccharomyces pombe|chr 1|||Manual Length = 325 Score = 27.1 bits (57), Expect = 4.0 Identities = 9/19 (47%), Positives = 15/19 (78%) Frame = +3 Query: 171 GNFFKDLEKMGQRVRDAVI 227 GNF KDL ++G+ + D++I Sbjct: 253 GNFVKDLSQLGRNLEDSII 271 >SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 2104 Score = 26.6 bits (56), Expect = 5.3 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 250 VSTAGAALMTASRTL*PIFSRSLKKLPGAAETEAVAKTIANTKTKIFENFILYFREIETQ 71 V T+ TAS+ + P F S+ G ETE + + + K K + + +RE Sbjct: 1580 VPTSPLKAPTASQLIIPNFDGSITNYSGEEETEWLQEEVNIMKIKELTSTVNKYREQLAM 1639 Query: 70 RTSCNE 53 S NE Sbjct: 1640 VQSLNE 1645 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,595,180 Number of Sequences: 5004 Number of extensions: 41380 Number of successful extensions: 358 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 242 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 497299314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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