Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_K19
(899 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5; Obtectomera|... 160 4e-38
UniRef50_P50725 Cluster: Attacin-A precursor; n=14; Obtectomera|... 144 3e-33
UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria cunea... 127 4e-28
UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia obliqua... 44 0.004
UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin ... 33 7.5
>UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5;
Obtectomera|Rep: Attacin-like protein - Antheraea
mylitta (Tasar silkworm)
Length = 230
Score = 160 bits (389), Expect = 4e-38
Identities = 85/182 (46%), Positives = 107/182 (58%)
Frame = +2
Query: 170 TRARRXAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRHKLSAASXGLALEQR 349
+R RR AG+ TVNSDGTSGAA+K+P+TGN+ + LSAIGS DFNDR+KL AA+ GLA +
Sbjct: 42 SRVRRQAGALTVNSDGTSGAAVKIPITGNENHKLSAIGSLDFNDRNKLGAATAGLAYDNV 101
Query: 350 KRARAESDRDPHSXXXXXXXXXXXXXXVPXXXXXXXXXXXXXXXTLPXPXPNAPNFXTLG 529
A + H + +P P PNF T+G
Sbjct: 102 NGHGATLTKT-HIPGFGDKMTAAGKVNLFHNDNHDLNANAFATRNMPN-IPQVPNFNTVG 159
Query: 530 GGVDYMFXQKVGASLSAAXSDVIXRNDYSAGGKLXLFRSPSXSLXFXAGFKXFXTPFYXS 709
GGVDYMF ++GAS SAA +D I RNDYS GGKL +F++P+ SL F AG+K F P Y S
Sbjct: 160 GGVDYMFKDRIGASASAAHTDFINRNDYSLGGKLNIFKTPTTSLDFNAGWKKFDMPSYRS 219
Query: 710 XW 715
W
Sbjct: 220 SW 221
Score = 39.5 bits (88), Expect = 0.11
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +3
Query: 372 TGTRIPGFGEXLGVAGXVXLFHNNXXDLSA 461
T T IPGFG+ + AG V LFHN+ DL+A
Sbjct: 109 TKTHIPGFGDKMTAAGKVNLFHNDNHDLNA 138
>UniRef50_P50725 Cluster: Attacin-A precursor; n=14;
Obtectomera|Rep: Attacin-A precursor - Trichoplusia ni
(Cabbage looper)
Length = 254
Score = 144 bits (349), Expect = 3e-33
Identities = 82/192 (42%), Positives = 102/192 (53%), Gaps = 1/192 (0%)
Frame = +2
Query: 173 RARRXA-GSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRHKLSAASXGLALEQR 349
R RR A GS T+NSDG+ G KVP+ GN+KNVLSA+GS D ND+ K ++ GLAL+
Sbjct: 59 RVRRQAQGSVTLNSDGSMGLGAKVPIVGNEKNVLSALGSVDLNDQLKPASRGMGLALDN- 117
Query: 350 KRARAESDRDPHSXXXXXXXXXXXXXXVPXXXXXXXXXXXXXXXTLPXPXPNAPNFXTLG 529
S V +P PN PNF T+G
Sbjct: 118 VNGHGLSVMKETVPGFGDRLTGAGRVNVFHNDNHDISAKAFVTKNMPD-FPNVPNFNTVG 176
Query: 530 GGVDYMFXQKVGASLSAAXSDVIXRNDYSAGGKLXLFRSPSXSLXFXAGFKXFXTPFYXS 709
GGVDYM+ KVGASL A + + R DYSA G L +FRSP+ S+ F AGFK F TP + S
Sbjct: 177 GGVDYMYKNKVGASLGMANTPFLDRKDYSAMGNLNVFRSPTTSVDFNAGFKKFDTPVFKS 236
Query: 710 XWXPXVGFSFSK 745
W P G +FS+
Sbjct: 237 NWEPNFGLTFSR 248
Score = 36.7 bits (81), Expect = 0.81
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +3
Query: 384 IPGFGEXLGVAGXVXLFHNNXXDLSAK 464
+PGFG+ L AG V +FHN+ D+SAK
Sbjct: 130 VPGFGDRLTGAGRVNVFHNDNHDISAK 156
>UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria
cunea|Rep: Putative attacin - Hyphantria cunea (Fall
webworm)
Length = 233
Score = 127 bits (306), Expect = 4e-28
Identities = 77/192 (40%), Positives = 97/192 (50%)
Frame = +2
Query: 173 RARRXAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRHKLSAASXGLALEQRK 352
RARR GS +N D TS A +K+PL G++KNVLSA+GS F+ LS+AS GLAL+
Sbjct: 44 RARRQLGSVFLNPDSTSRANIKLPLAGSNKNVLSALGSVGFDANKHLSSASGGLALDN-V 102
Query: 353 RARAESDRDPHSXXXXXXXXXXXXXXVPXXXXXXXXXXXXXXXTLPXPXPNAPNFXTLGG 532
R S H + +P P PNF T+G
Sbjct: 103 RGHGLSLTGTHIPNFGNQLTGAGRLNLFHNQNHDLNANAFLTRNMPT-IPQVPNFNTVGS 161
Query: 533 GVDYMFXQKVGASLSAAXSDVIXRNDYSAGGKLXLFRSPSXSLXFXAGFKXFXTPFYXSX 712
++YMF KVGASL A+ + + R DYSA G L LFR+PS SL F AG +PF S
Sbjct: 162 -LNYMFKNKVGASLGASRTPFLQRTDYSANGNLNLFRNPSTSLDFNAGVSKSVSPFMQSS 220
Query: 713 WXPXVGFSFSKF 748
W P G SK+
Sbjct: 221 WLPNFGLRLSKY 232
Score = 37.5 bits (83), Expect = 0.46
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = +3
Query: 372 TGTRIPGFGEXLGVAGXVXLFHNNXXDLSA 461
TGT IP FG L AG + LFHN DL+A
Sbjct: 110 TGTHIPNFGNQLTGAGRLNLFHNQNHDLNA 139
>UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia
obliqua|Rep: Defense protein 2 - Lonomia obliqua (Moth)
Length = 113
Score = 44.4 bits (100), Expect = 0.004
Identities = 23/77 (29%), Positives = 35/77 (45%)
Frame = +2
Query: 500 PNAPNFXTLGGGVDYMFXQKVGASLSAAXSDVIXRNDYSAGGKLXLFRSPSXSLXFXAGF 679
PN ++ +DY++ K+ ASL A S ++ R D SA GK+ L + L G
Sbjct: 30 PNLSDYNKYSAILDYLYKDKLSASLGVAHSGLLDRTDLSALGKVNLLNDKNTRLDLFGGL 89
Query: 680 KXFXTPFYXSXWXPXVG 730
+P + S P G
Sbjct: 90 TKSMSPKFDSGLKPNFG 106
>UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin -
Musca domestica (House fly)
Length = 208
Score = 33.5 bits (73), Expect = 7.5
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +2
Query: 503 NAPNFXTLGGGVDYMFXQKVGASLSAAXSDVIXRNDYSAGGKLXLFRS 646
N F T+GGG+DY GAS++A+ + N GK L++S
Sbjct: 124 NGFKFNTVGGGLDYNHANGHGASVTASRIPQLNMNTVDVTGKANLWKS 171
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 504,687,055
Number of Sequences: 1657284
Number of extensions: 6992461
Number of successful extensions: 15751
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 15299
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15744
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 81571813589
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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