BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_J24 (981 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 36 0.009 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.14 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.19 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.76 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 29 1.0 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 28 2.3 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.1 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 4.0 SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3||... 26 7.1 SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|c... 26 7.1 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 9.3 SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 26 9.3 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 35.9 bits (79), Expect = 0.009 Identities = 15/42 (35%), Positives = 17/42 (40%) Frame = +1 Query: 856 PPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXPXXXP 981 PP P S +P + P P PPPP PP P P Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAP 1731 Score = 31.9 bits (69), Expect = 0.14 Identities = 14/36 (38%), Positives = 14/36 (38%) Frame = +1 Query: 832 PXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPP 939 P P PP P S P P A P P PPP Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Score = 29.1 bits (62), Expect = 1.0 Identities = 19/51 (37%), Positives = 20/51 (39%) Frame = +2 Query: 794 PXXPXXXXPXPXXPAXXPFPPXXXXPXLFPPPPAXGXXPXPPXXXXXPPPP 946 P P P P A P PP P PPPP+ P P PPPP Sbjct: 1691 PVRPQSAAP-PQMSAPTPPPP----PMSVPPPPS--APPMPAGPPSAPPPP 1734 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 31.9 bits (69), Expect = 0.14 Identities = 15/41 (36%), Positives = 15/41 (36%) Frame = +1 Query: 832 PXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPP 954 P P P PP P P P P PPPP PP Sbjct: 744 PAPAPIPVPPPAPIMGGP-PPPPPPPGVAGAGPPPPPPPPP 783 Score = 31.5 bits (68), Expect = 0.19 Identities = 21/55 (38%), Positives = 21/55 (38%), Gaps = 4/55 (7%) Frame = +2 Query: 794 PXXPXXXXPXPXXPAXXPFPP----XXXXPXLFPPPPAXGXXPXPPXXXXXPPPP 946 P P P P PA P PP P PPP G P PP PPPP Sbjct: 734 PPPPAVIVPTPA-PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP----PPPPP 783 Score = 27.5 bits (58), Expect = 3.1 Identities = 16/46 (34%), Positives = 17/46 (36%), Gaps = 2/46 (4%) Frame = +1 Query: 823 PXXPXPTXXPXP-PXXPPS-XSPXPXAXXXPXXPXXXXPPPPXXPP 954 P PT P P P PP+ P P PPPP PP Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 27.1 bits (57), Expect = 4.0 Identities = 16/47 (34%), Positives = 17/47 (36%), Gaps = 4/47 (8%) Frame = +2 Query: 818 PXPXXPAXXPFPPXXXXPXLFPPPPAXGXXPXPP----XXXXXPPPP 946 P P PA P + PP P G P PP PPPP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778 Score = 26.2 bits (55), Expect = 7.1 Identities = 11/34 (32%), Positives = 13/34 (38%) Frame = +1 Query: 850 PXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXP 951 P P P+ +P P P PPPP P Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.5 bits (68), Expect = 0.19 Identities = 21/86 (24%), Positives = 23/86 (26%), Gaps = 3/86 (3%) Frame = +1 Query: 733 PXGXPP---PXXXPDXXLXFXXXXGXPXXXXXXPXXPXPTXXPXPPXXPPSXSPXPXAXX 903 P G PP P P P P P P+ P P + P P Sbjct: 1079 PSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSG 1138 Query: 904 XPXXPXXXXPPPPXXPPXXXXPXXXP 981 P P PP P P P Sbjct: 1139 APPVPKPSVAAPPVPAPSGAPPVPKP 1164 Score = 28.3 bits (60), Expect = 1.7 Identities = 17/53 (32%), Positives = 17/53 (32%), Gaps = 2/53 (3%) Frame = +2 Query: 794 PXXPXXXXPXPXXPAXXPFPPXXXXPXLFPPPPA--XGXXPXPPXXXXXPPPP 946 P P P PA PP PP PA G P P PP P Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVP 1192 Score = 27.5 bits (58), Expect = 3.1 Identities = 21/83 (25%), Positives = 26/83 (31%), Gaps = 2/83 (2%) Frame = +2 Query: 704 PPPXXQXPXX-PXGXPPLXXXRTXXSFXRQXPXXPXXXXPXPXXPAXXPFPPXXXXPXLF 880 P P P P PP+ ++ P P P PA PP + Sbjct: 1035 PVPSTAPPVPIPTSTPPVP--KSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIP 1092 Query: 881 P-PPPAXGXXPXPPXXXXXPPPP 946 P P P+ P P PP P Sbjct: 1093 PVPKPSVAAPPVPKPSVAVPPVP 1115 Score = 27.5 bits (58), Expect = 3.1 Identities = 16/52 (30%), Positives = 16/52 (30%), Gaps = 1/52 (1%) Frame = +2 Query: 794 PXXPXXXXPXPXXPAXXPFPPXXXXPXLFPPPPAX-GXXPXPPXXXXXPPPP 946 P P P P PP PP PA G P P PP P Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVP 1172 Score = 27.5 bits (58), Expect = 3.1 Identities = 14/56 (25%), Positives = 16/56 (28%) Frame = +1 Query: 802 PXXXXXXPXXPXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXP 969 P P P P+ P P + P P P P PP P P Sbjct: 1124 PKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIP 1179 Score = 27.5 bits (58), Expect = 3.1 Identities = 19/88 (21%), Positives = 20/88 (22%) Frame = +1 Query: 706 PPXXTTPXXPXGXPPPXXXPDXXLXFXXXXGXPXXXXXXPXXPXPTXXPXPPXXPPSXSP 885 PP P P P P P P P+ P P P Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209 Query: 886 XPXAXXXPXXPXXXXPPPPXXPPXXXXP 969 P P P PP P P Sbjct: 1210 VPPPSTAPPVPTPSAGLPPVPVPTAKAP 1237 Score = 27.1 bits (57), Expect = 4.0 Identities = 16/61 (26%), Positives = 17/61 (27%), Gaps = 1/61 (1%) Frame = +1 Query: 802 PXXXXXXPXXPXPTXX-PXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXPXXX 978 P P P P+ P P P P P P PP PP P Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPT 1221 Query: 979 P 981 P Sbjct: 1222 P 1222 Score = 26.2 bits (55), Expect = 7.1 Identities = 16/53 (30%), Positives = 19/53 (35%), Gaps = 2/53 (3%) Frame = +2 Query: 794 PXXPXXXXPXPXXPAXX--PFPPXXXXPXLFPPPPAXGXXPXPPXXXXXPPPP 946 P P P P+ P PP P + P P+ G P P PP P Sbjct: 1190 PVPPPSEAPPVPKPSVGVPPVPPPSTAPPV--PTPSAGLPPVPVPTAKAPPVP 1240 Score = 25.8 bits (54), Expect = 9.3 Identities = 13/51 (25%), Positives = 15/51 (29%) Frame = +1 Query: 802 PXXXXXXPXXPXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPP 954 P P P P+ P P + P P P P P PP Sbjct: 1015 PKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPP 1065 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 29.5 bits (63), Expect = 0.76 Identities = 13/25 (52%), Positives = 13/25 (52%), Gaps = 3/25 (12%) Frame = +2 Query: 881 PPPP---AXGXXPXPPXXXXXPPPP 946 PPPP A G P PP PPPP Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPP 364 Score = 27.9 bits (59), Expect = 2.3 Identities = 22/85 (25%), Positives = 24/85 (28%), Gaps = 4/85 (4%) Frame = +2 Query: 704 PPPXXQXPXXPXGXPPLXXXRTXXSFXRQXPXXPXXXXP--XPXXPAXXPFPPXXXXPXL 877 PPP P PPL R + P P P P A P P Sbjct: 363 PPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPS 422 Query: 878 FPP--PPAXGXXPXPPXXXXXPPPP 946 PP PP+ P P P Sbjct: 423 LPPSAPPSLPPSAPPSLPMGAPAAP 447 Score = 27.9 bits (59), Expect = 2.3 Identities = 16/50 (32%), Positives = 16/50 (32%) Frame = +1 Query: 832 PXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXPXXXP 981 P PT PP PPS P P P PP P P P Sbjct: 416 PVPTPPSLPPSAPPSL-PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMP 464 Score = 27.5 bits (58), Expect = 3.1 Identities = 20/72 (27%), Positives = 20/72 (27%) Frame = +2 Query: 704 PPPXXQXPXXPXGXPPLXXXRTXXSFXRQXPXXPXXXXPXPXXPAXXPFPPXXXXPXLFP 883 P P P P PP P P P PA P P P Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPI-APPLPAGMPAAP-PLPPAAPA 475 Query: 884 PPPAXGXXPXPP 919 PPPA P P Sbjct: 476 PPPAPAPAPAAP 487 Score = 26.6 bits (56), Expect = 5.3 Identities = 23/98 (23%), Positives = 26/98 (26%), Gaps = 6/98 (6%) Frame = +1 Query: 706 PPXXTTPXXPXGXPPPXXXPDXXLXFXXXXGXPXXXXXXPXXPXPTXXP-XPPXXPPSXS 882 PP ++ PP P G P P+ P PP PPS Sbjct: 377 PPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAP 436 Query: 883 P-----XPXAXXXPXXPXXXXPPPPXXPPXXXXPXXXP 981 P P A P P P P P P Sbjct: 437 PSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474 Score = 26.2 bits (55), Expect = 7.1 Identities = 16/59 (27%), Positives = 17/59 (28%), Gaps = 1/59 (1%) Frame = +1 Query: 709 PXXTTPXXPXGXPP-PXXXPDXXLXFXXXXGXPXXXXXXPXXPXPTXXPXPPXXPPSXS 882 P P P G P P P + G P P P P P P P S Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVAS 490 Score = 25.8 bits (54), Expect = 9.3 Identities = 19/78 (24%), Positives = 19/78 (24%) Frame = +1 Query: 709 PXXTTPXXPXGXPPPXXXPDXXLXFXXXXGXPXXXXXXPXXPXPTXXPXPPXXPPSXSPX 888 P P PPP P P P PP PPS S Sbjct: 241 PPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPS-SRV 299 Query: 889 PXAXXXPXXPXXXXPPPP 942 A PPPP Sbjct: 300 SAAALAANKKRPPPPPPP 317 Score = 25.8 bits (54), Expect = 9.3 Identities = 15/60 (25%), Positives = 16/60 (26%) Frame = +1 Query: 802 PXXXXXXPXXPXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXPXXXP 981 P P P+ P PP P A P P PP P P P Sbjct: 425 PSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 29.1 bits (62), Expect = 1.0 Identities = 12/29 (41%), Positives = 12/29 (41%) Frame = +1 Query: 868 PPSXSPXPXAXXXPXXPXXXXPPPPXXPP 954 PPS P A P PPPP PP Sbjct: 168 PPSFQPPSAAAPATSLPSDYNPPPPPPPP 196 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 27.9 bits (59), Expect = 2.3 Identities = 15/48 (31%), Positives = 15/48 (31%), Gaps = 1/48 (2%) Frame = +1 Query: 841 TXXPXPPXX-PPSXSPXPXAXXXPXXPXXXXPPPPXXPPXXXXPXXXP 981 T P PP P P P P P PP PP P P Sbjct: 489 TSAPLPPTTFAPPGVPLPPIPGAPGMPNLNMSQPPMVPPGMALPPGMP 536 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.5 bits (58), Expect = 3.1 Identities = 14/48 (29%), Positives = 15/48 (31%) Frame = +2 Query: 803 PXXXXPXPXXPAXXPFPPXXXXPXLFPPPPAXGXXPXPPXXXXXPPPP 946 P P P+ P P P PP PP PPPP Sbjct: 158 PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205 Score = 27.1 bits (57), Expect = 4.0 Identities = 14/50 (28%), Positives = 16/50 (32%) Frame = +1 Query: 802 PXXXXXXPXXPXPTXXPXPPXXPPSXSPXPXAXXXPXXPXXXXPPPPXXP 951 P P P P+ PP P P P + P P PP P Sbjct: 140 PTSAPPRPSIPPPSPASAPPI-PSKAPPIPSSLPPPAQPAAPVKSPPSAP 188 Score = 26.2 bits (55), Expect = 7.1 Identities = 16/46 (34%), Positives = 16/46 (34%), Gaps = 2/46 (4%) Frame = +1 Query: 823 PXXPXPTXXPXPPXX-PPSXS-PXPXAXXXPXXPXXXXPPPPXXPP 954 P P P PP PP S P P P P P P PP Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPP 173 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 27.1 bits (57), Expect = 4.0 Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 2/58 (3%) Frame = +3 Query: 144 KMAVNSATSFVLSSILTLLIFSGMQMYKPQLILSPMTIIFGGYLG--SLMFMFFVTAV 311 +M +NS + S LT+ + +G+ Y LS +I GYL S+ F+ V +V Sbjct: 323 QMRINSPSHVQRLSFLTIAMQAGLDAYIAIFFLSTNAVIEKGYLPFVSVAFLSLVPSV 380 >SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 465 Score = 26.2 bits (55), Expect = 7.1 Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Frame = +3 Query: 273 LGSLMFMFFVTAVGNLETILFGKAFQLKLPEVVL-SMGISLIAAGMVHRICFTTCLIF 443 L L F F V + ++L G + LP ++L ++ I +AA + FTTCLI+ Sbjct: 131 LTGLCFATFSYEVSPVVSVLSG-VLLISLPTLILLNLCILKLAAKLHLSALFTTCLIY 187 >SPAC13A11.05 |||peptidase family M17|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 26.2 bits (55), Expect = 7.1 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = -3 Query: 355 FNWNALPNRIVSRLPTAVTK 296 FNW++L + ++ R+P A TK Sbjct: 11 FNWSSLSSILLPRIPLATTK 30 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 25.8 bits (54), Expect = 9.3 Identities = 11/26 (42%), Positives = 11/26 (42%) Frame = +2 Query: 824 PXXPAXXPFPPXXXXPXLFPPPPAXG 901 P P P PP P PPPP G Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPPG 31 Score = 25.8 bits (54), Expect = 9.3 Identities = 11/22 (50%), Positives = 11/22 (50%) Frame = +2 Query: 881 PPPPAXGXXPXPPXXXXXPPPP 946 PPPP G P P PPPP Sbjct: 11 PPPPPPGFEP--PSQPPPPPPP 30 >SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 624 Score = 25.8 bits (54), Expect = 9.3 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = -3 Query: 187 IDDSTKDVALFTAILPYFIWSNLCQLVFLLSLT*MTGLFRLMILSLTQN 41 +D++T + ++ P+ +SN+ ++ T +G+ LS TQN Sbjct: 488 VDNNTPPLLVYFPNYPWVYYSNISTFTMSMNDTLSSGILENAALSATQN 536 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,989,252 Number of Sequences: 5004 Number of extensions: 59363 Number of successful extensions: 295 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 228 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 505288058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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