Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_J15
(945 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 1.1
At1g62250.2 68414.m07023 expressed protein 30 1.9
At1g62250.1 68414.m07022 expressed protein 30 1.9
At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid t... 30 2.6
At4g22670.1 68417.m03272 tetratricopeptide repeat (TPR)-containi... 29 3.4
At5g09400.1 68418.m01089 potassium transporter family protein si... 29 4.5
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 29 4.5
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 5.9
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 5.9
At1g69220.2 68414.m07926 serine/threonine protein kinase, putati... 28 7.8
At1g69220.1 68414.m07925 serine/threonine protein kinase, putati... 28 7.8
At1g47310.1 68414.m05238 expressed protein 28 7.8
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 31.1 bits (67), Expect = 1.1
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = +1
Query: 211 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 384
+G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557
Query: 385 --PIQFRVIFTEQTVKLINKRDHHA 453
P+ F +F + L++ + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582
>At1g62250.2 68414.m07023 expressed protein
Length = 223
Score = 30.3 bits (65), Expect = 1.9
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +2
Query: 107 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 235
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62250.1 68414.m07022 expressed protein
Length = 267
Score = 30.3 bits (65), Expect = 1.9
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +2
Query: 107 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 235
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62500.1 68414.m07052 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to auxin
down regulated GB:X69640 GI:296442 from [Glycine max];
contains Pfam profile PF00234: Protease inhibitor/seed
storage/LTP family
Length = 297
Score = 29.9 bits (64), Expect = 2.6
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = -1
Query: 918 GTRXXHGXXGGGXPXEXGGGXGG 850
G HG GGG P GGG GG
Sbjct: 63 GKPPPHGGKGGGPPHHGGGGGGG 85
>At4g22670.1 68417.m03272 tetratricopeptide repeat (TPR)-containing
protein similar to Hsc70-interacting protein (Hip) from
{Homo sapiens} SP|P50502, {Rattus norvegicus} SP|P50503;
contains Pfam profile PF00515: tetratricopeptide repeat
(TPR) domain
Length = 441
Score = 29.5 bits (63), Expect = 3.4
Identities = 14/33 (42%), Positives = 14/33 (42%)
Frame = -3
Query: 901 RXXGGGFXXRKXGGXXGNFXXGAPGGXXGFWXG 803
R GGGF GG G G PGG G G
Sbjct: 287 RPSGGGFPGGMPGGFPGGMPGGFPGGMGGMPGG 319
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 29.1 bits (62), Expect = 4.5
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -2
Query: 293 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 114
F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468
Query: 113 NKVSLILAQWLSL 75
++ + + W L
Sbjct: 469 GQIYIPVLNWFLL 481
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 29.1 bits (62), Expect = 4.5
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +1
Query: 370 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 546
V S + +QF V + + IN R D HAL ++ + + +K+ F D + + F P
Sbjct: 76 VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135
Query: 547 VLENNRV 567
E R+
Sbjct: 136 KDERPRL 142
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 28.7 bits (61), Expect = 5.9
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -3
Query: 355 PLSITGRRSPWCSSCRFRSDASR 287
PLS GR S +C+SC+ R D +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 28.7 bits (61), Expect = 5.9
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -1
Query: 453 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 361
G +P + +D + G+DH + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251
>At1g69220.2 68414.m07926 serine/threonine protein kinase, putative
identical to serine/threonine kinase [Arabidopsis
thaliana] gi|2352084|gb|AAB68776
Length = 809
Score = 28.3 bits (60), Expect = 7.8
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -1
Query: 132 HGEDEGKQSQSHLGAVVIFESKSTNTVKILREFP 31
HGEDE ++S S V ES S+ + REFP
Sbjct: 571 HGEDETEESDSRSQLVREKESSSSQFEGVPREFP 604
>At1g69220.1 68414.m07925 serine/threonine protein kinase, putative
identical to serine/threonine kinase [Arabidopsis
thaliana] gi|2352084|gb|AAB68776
Length = 836
Score = 28.3 bits (60), Expect = 7.8
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -1
Query: 132 HGEDEGKQSQSHLGAVVIFESKSTNTVKILREFP 31
HGEDE ++S S V ES S+ + REFP
Sbjct: 598 HGEDETEESDSRSQLVREKESSSSQFEGVPREFP 631
>At1g47310.1 68414.m05238 expressed protein
Length = 395
Score = 28.3 bits (60), Expect = 7.8
Identities = 19/67 (28%), Positives = 35/67 (52%)
Frame = +1
Query: 409 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 588
T QTV + + A ++ Q + + G ++ SKKV+W+ P +E RV+F++ +
Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342
Query: 589 EDKQYLK 609
+ LK
Sbjct: 343 IEGDKLK 349
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,886,137
Number of Sequences: 28952
Number of extensions: 371979
Number of successful extensions: 1551
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1534
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2266029384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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