BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_J14 (893 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 31 0.29 SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 29 0.89 SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosacc... 27 2.7 SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 27 2.7 SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi... 27 3.6 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 30.7 bits (66), Expect = 0.29 Identities = 16/55 (29%), Positives = 31/55 (56%), Gaps = 2/55 (3%) Frame = +2 Query: 89 IMASKFKSEKIGIVGSGLIGRSWA--MLFASVGYQVTVYDVVAKQITDAIEDIKY 247 + +S FKS KI IVG+G +G + A +L + + ++ + D+ K+ D+ + Sbjct: 13 VRSSSFKSIKIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNH 67 >SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 29.1 bits (62), Expect = 0.89 Identities = 11/36 (30%), Positives = 22/36 (61%) Frame = +2 Query: 101 KFKSEKIGIVGSGLIGRSWAMLFASVGYQVTVYDVV 208 + + + +GI+G G IG ++L ++G V YD++ Sbjct: 192 EIRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYDIL 227 >SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosaccharomyces pombe|chr 3|||Manual Length = 431 Score = 27.5 bits (58), Expect = 2.7 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +2 Query: 116 KIGIVGSGLIGRSWAMLFASVGYQVTVYD 202 ++GI+G G +GR +A + G++V V D Sbjct: 6 QVGIIGFGDMGRLYAEYISKAGWRVNVCD 34 >SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 27.5 bits (58), Expect = 2.7 Identities = 9/30 (30%), Positives = 20/30 (66%) Frame = +2 Query: 113 EKIGIVGSGLIGRSWAMLFASVGYQVTVYD 202 +++GI+G G IG+S+A +G ++ ++ Sbjct: 160 KRVGIIGMGAIGKSFAQKILPLGCEIVYHN 189 >SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schizosaccharomyces pombe|chr 1|||Manual Length = 511 Score = 27.1 bits (57), Expect = 3.6 Identities = 12/43 (27%), Positives = 26/43 (60%) Frame = +2 Query: 74 VASTVIMASKFKSEKIGIVGSGLIGRSWAMLFASVGYQVTVYD 202 V+ST ++ +K+ ++G G+IG +++ +G +VTV + Sbjct: 205 VSSTGALSLSEVPKKMTVLGGGIIGLEMGSVWSRLGAEVTVVE 247 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,191,683 Number of Sequences: 5004 Number of extensions: 64247 Number of successful extensions: 168 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 450492750 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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