BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_J13 (951 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.31 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.31 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.55 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 29 0.96 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.9 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.1 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 5.1 SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 9.0 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 9.0 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 30.7 bits (66), Expect = 0.31 Identities = 35/133 (26%), Positives = 35/133 (26%), Gaps = 4/133 (3%) Frame = +3 Query: 552 PNXXXPXXXLGPPPPPXXXAPXXPXFXXXXTPGHXPGXXLXPXXXPP-PXPXFXPP-PXX 725 P P P P P P P P P PP P P PP P Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVP------APSGAPPVPKPSVAAPPVPVPSGAPPVPKP 1145 Query: 726 XFXLXXPXXPXGX--VPXXXXPAXTXXAPXXXXPXXXXFXFFXAPXPXPPXXPXPXPXPX 899 P G VP A AP P P P P P P P Sbjct: 1146 SVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAP---PVPK 1202 Query: 900 XXAXXXPXPPPPT 938 P PPP T Sbjct: 1203 PSVGVPPVPPPST 1215 Score = 26.6 bits (56), Expect = 5.1 Identities = 31/140 (22%), Positives = 35/140 (25%), Gaps = 3/140 (2%) Frame = +1 Query: 526 PPXXXVXGXPTXXXPXXXWAPPPPPXXXXPXPPSFXXAXPPXTXLAXIWXPXXXPXXXPX 705 PP P APP P P P A PP + P P Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAP----- 1117 Query: 706 SXPPPXXLSXXXFPXXPXXRFXXXXXXXXXXSPPXXA---XPXXXXXXFXGXPXXXPXXX 876 S PP P P +PP A P P P Sbjct: 1118 SGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSG 1177 Query: 877 XXPXPXPSXXXXXXPXLXPP 936 P P P+ P + PP Sbjct: 1178 IPPVPKPA---AGVPPVPPP 1194 Score = 26.2 bits (55), Expect = 6.8 Identities = 21/90 (23%), Positives = 23/90 (25%) Frame = +3 Query: 552 PNXXXPXXXLGPPPPPXXXAPXXPXFXXXXTPGHXPGXXLXPXXXPPPXPXFXPPPXXXF 731 P P + P P P P P P P PP P PP Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPP------PSEAPPVPKPSVGVPPVPPPSTAPPVPTP 1222 Query: 732 XLXXPXXPXGXVPXXXXPAXTXXAPXXXXP 821 P P PA + AP P Sbjct: 1223 SAGLPPVPVPTAKAPPVPAPSSEAPSVSTP 1252 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 30.7 bits (66), Expect = 0.31 Identities = 14/45 (31%), Positives = 16/45 (35%) Frame = +1 Query: 586 PPPPPXXXXPXPPSFXXAXPPXTXLAXIWXPXXXPXXXPXSXPPP 720 PPPPP P P PP + P P + PPP Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777 Score = 29.1 bits (62), Expect = 0.96 Identities = 11/27 (40%), Positives = 11/27 (40%) Frame = +3 Query: 852 PXPXPPXXPXPXPXPXXXAXXXPXPPP 932 P P P P P P P P PPP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPP 768 Score = 29.1 bits (62), Expect = 0.96 Identities = 11/26 (42%), Positives = 11/26 (42%) Frame = +3 Query: 858 PXPPXXPXPXPXPXXXAXXXPXPPPP 935 P P P P P P P PPPP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPP 767 Score = 28.3 bits (60), Expect = 1.7 Identities = 14/35 (40%), Positives = 14/35 (40%), Gaps = 6/35 (17%) Frame = +3 Query: 849 APXPXPPXXPX------PXPXPXXXAXXXPXPPPP 935 AP P PP P P P P P PPPP Sbjct: 747 APIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781 Score = 25.8 bits (54), Expect = 9.0 Identities = 11/29 (37%), Positives = 11/29 (37%) Frame = +3 Query: 849 APXPXPPXXPXPXPXPXXXAXXXPXPPPP 935 AP P P P P P PPPP Sbjct: 755 APIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 29.9 bits (64), Expect = 0.55 Identities = 30/123 (24%), Positives = 31/123 (25%), Gaps = 2/123 (1%) Frame = +3 Query: 567 PXXXLGPPPPPXXXAPXXPXFXXXXTPGHXPGXXLXPXXXPPPXPXFXPPPXXXFXLXXP 746 P PPPPP AP P P PP P P Sbjct: 355 PQGRSAPPPPPPRSAPST---GRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLG---N 408 Query: 747 XXPXGXVPXXXXPAXTXXAPXXXXPXXXXFXFFXAPX--PXPPXXPXPXPXPXXXAXXXP 920 P P+ AP P AP P PP P P P P Sbjct: 409 ASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468 Query: 921 XPP 929 PP Sbjct: 469 LPP 471 Score = 29.5 bits (63), Expect = 0.73 Identities = 27/104 (25%), Positives = 27/104 (25%) Frame = +2 Query: 584 PPPPPXXPXXXXPXLFXXXNPRXPPWPXFGXPFXTPXXTPXXXPPXXXFLXXXSXGPRGX 763 P PPP P P L N P P TP P PP S P Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTP----PVPTPPSLPPSAPP--------SLPPSAP 436 Query: 764 GSXPXXXXXNXXRPXPXPXPXXXLXXFXGXXXXXPXXPXPPPPP 895 S P P P P P PPP P Sbjct: 437 PSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480 Score = 26.2 bits (55), Expect = 6.8 Identities = 29/121 (23%), Positives = 32/121 (26%), Gaps = 3/121 (2%) Frame = +3 Query: 585 PPPPPXXXAPXXPXFXXXXTPGHXPGXXLXPXXXPPPXPXFXPPPXXXFXLXXPXXPXGX 764 PPPPP P + P PPP PPP P P G Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLP---------PPP-----PPPRSNAAGSIPLPPQGR 358 Query: 765 VPXXXXPAXTXXAPXXXXPXXXXFXFFXAPXPXPPXXPX---PXPXPXXXAXXXPXPPPP 935 P + + P P PP P P P A PP P Sbjct: 359 SAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVP 418 Query: 936 T 938 T Sbjct: 419 T 419 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 29.1 bits (62), Expect = 0.96 Identities = 19/65 (29%), Positives = 21/65 (32%) Frame = +3 Query: 525 SPLXXGXWXPNXXXPXXXLGPPPPPXXXAPXXPXFXXXXTPGHXPGXXLXPXXXPPPXPX 704 +P G P G P PP AP P P PG L P P P P Sbjct: 483 APFIPGTSAPLPPTTFAPPGVPLPPIPGAPGMPNLNMSQPPMVPPGMAL-PPGMPAPFPG 541 Query: 705 FXPPP 719 + P Sbjct: 542 YPAVP 546 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 27.5 bits (58), Expect = 2.9 Identities = 17/50 (34%), Positives = 18/50 (36%) Frame = -3 Query: 715 GGXXWGXGGGXKXGXKXXPGXXPGVXXXKKXGXXGAXXXGGGGGPXXXKG 566 GG G GGG G PG G G G GGGP +G Sbjct: 187 GGGFGGFGGG-SGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEG 235 Score = 25.8 bits (54), Expect = 9.0 Identities = 19/63 (30%), Positives = 21/63 (33%) Frame = -3 Query: 754 GXXGXXXKKXXXGGGXXWGXGGGXKXGXKXXPGXXPGVXXXKKXGXXGAXXXGGGGGPXX 575 G G + GG +G G G G G PG G G G GGGP Sbjct: 209 GFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPG-GFGGGLGGFGGGPGGFGGGPGG 267 Query: 574 XKG 566 G Sbjct: 268 HGG 270 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.6 bits (56), Expect = 5.1 Identities = 13/31 (41%), Positives = 13/31 (41%), Gaps = 2/31 (6%) Frame = +3 Query: 849 APXPXPP--XXPXPXPXPXXXAXXXPXPPPP 935 AP P PP P P P A PPPP Sbjct: 1704 APTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734 Score = 25.8 bits (54), Expect = 9.0 Identities = 19/57 (33%), Positives = 19/57 (33%), Gaps = 1/57 (1%) Frame = +1 Query: 553 PTXXXPXXXWAP-PPPPXXXXPXPPSFXXAXPPXTXLAXIWXPXXXPXXXPXSXPPP 720 P P AP PPPP P PPS PP P P S P P Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPS----APPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 26.6 bits (56), Expect = 5.1 Identities = 10/24 (41%), Positives = 10/24 (41%) Frame = +3 Query: 864 PPXXPXPXPXPXXXAXXXPXPPPP 935 PP P P P P PPPP Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28 Score = 26.2 bits (55), Expect = 6.8 Identities = 10/24 (41%), Positives = 10/24 (41%) Frame = +3 Query: 864 PPXXPXPXPXPXXXAXXXPXPPPP 935 P P P P P P PPPP Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPP 29 >SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 296 Score = 25.8 bits (54), Expect = 9.0 Identities = 12/44 (27%), Positives = 16/44 (36%) Frame = +1 Query: 583 APPPPPXXXXPXPPSFXXAXPPXTXLAXIWXPXXXPXXXPXSXP 714 A PPPP PP+ + PP + P P + P Sbjct: 204 AQPPPPPPQQNYPPAASSSAPPMQYQQTAYPPQQAPYPPVQAYP 247 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 25.8 bits (54), Expect = 9.0 Identities = 20/71 (28%), Positives = 22/71 (30%), Gaps = 4/71 (5%) Frame = +1 Query: 517 AXXPPXXXVXGXPTXXXPXXXWAPPPPPXXXXP----XPPSFXXAXPPXTXLAXIWXPXX 684 A PP P P PPP P P PP PP A + P Sbjct: 127 APAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPS 186 Query: 685 XPXXXPXSXPP 717 P P + PP Sbjct: 187 AP-SLPSAVPP 196 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,857,480 Number of Sequences: 5004 Number of extensions: 24981 Number of successful extensions: 167 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 51 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 485316198 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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