Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_J11
(922 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 134 1e-32
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 134 1e-32
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 81 2e-16
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 53 7e-08
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 4e-05
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 40 7e-04
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 36 0.006
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 36 0.008
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.033
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.033
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.033
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.13
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.53
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.6
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 2.1
SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pom... 27 4.9
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.5
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 6.5
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 134 bits (325), Expect = 1e-32
Identities = 75/159 (47%), Positives = 98/159 (61%)
Frame = +3
Query: 123 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 302
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 303 KDEQDRCITIKSTAISMFFELEEKDLVFITNPEPA*KE*ERFLDQLD*LTWNTLISLLK* 482
DEQ+R +TIKSTAIS+F E+ + D+ + P +D + +++ ++
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTDFLVNLIDSPGHVDFSSEVTAA-- 118
Query: 483 QLHSVFTDGALXXXXXXXXXXXQTETVXXQAIAXRIXPL 599
TDGAL QTETV QA+ RI P+
Sbjct: 119 ---LRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPV 154
Score = 34.7 bits (76), Expect = 0.019
Identities = 27/82 (32%), Positives = 35/82 (42%)
Frame = +2
Query: 458 HVDFSSEVTAALRVH*WSPCGC*LCVWCVCTN*NSTSSGYCXAHXTPILXMNKMDRXXXX 637
HVDFSSEVTAALRV + + VC + P++ +NK+DR
Sbjct: 108 HVDFSSEVTAALRVTDGALVVV-DTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLE 166
Query: 638 XXXXXXXXYHTSHRXVEXVXVL 703
Y R VE V V+
Sbjct: 167 LQISQEELYQNFARVVESVNVV 188
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 134 bits (325), Expect = 1e-32
Identities = 75/159 (47%), Positives = 98/159 (61%)
Frame = +3
Query: 123 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 302
MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60
Query: 303 KDEQDRCITIKSTAISMFFELEEKDLVFITNPEPA*KE*ERFLDQLD*LTWNTLISLLK* 482
DEQ+R +TIKSTAIS+F E+ + D+ + P +D + +++ ++
Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDMKDMKEPADGTDFLVNLIDSPGHVDFSSEVTAA-- 118
Query: 483 QLHSVFTDGALXXXXXXXXXXXQTETVXXQAIAXRIXPL 599
TDGAL QTETV QA+ RI P+
Sbjct: 119 ---LRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPV 154
Score = 34.7 bits (76), Expect = 0.019
Identities = 27/82 (32%), Positives = 35/82 (42%)
Frame = +2
Query: 458 HVDFSSEVTAALRVH*WSPCGC*LCVWCVCTN*NSTSSGYCXAHXTPILXMNKMDRXXXX 637
HVDFSSEVTAALRV + + VC + P++ +NK+DR
Sbjct: 108 HVDFSSEVTAALRVTDGALVVV-DTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLE 166
Query: 638 XXXXXXXXYHTSHRXVEXVXVL 703
Y R VE V V+
Sbjct: 167 LQISQEELYQNFARVVESVNVV 188
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 81.4 bits (192), Expect = 2e-16
Identities = 37/75 (49%), Positives = 55/75 (73%)
Frame = +3
Query: 141 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 320
+++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R
Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66
Query: 321 CITIKSTAISMFFEL 365
IT+KS+AIS+FF++
Sbjct: 67 GITMKSSAISLFFKV 81
Score = 29.9 bits (64), Expect = 0.53
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +1
Query: 400 NQREKSEKGFLINLIDSPG 456
N ++ EK +LINLIDSPG
Sbjct: 85 NDEKRVEKDYLINLIDSPG 103
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 52.8 bits (121), Expect = 7e-08
Identities = 27/71 (38%), Positives = 43/71 (60%)
Frame = +3
Query: 147 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 326
+RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I
Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106
Query: 327 TIKSTAISMFF 359
T+K+ SM +
Sbjct: 107 TVKAQTCSMIY 117
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 4e-05
Identities = 26/73 (35%), Positives = 40/73 (54%), Gaps = 3/73 (4%)
Frame = +3
Query: 174 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCITIKSTA 344
+IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R ITI S A
Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITINSAA 85
Query: 345 ISMFFELEEKDLV 383
IS + + +L+
Sbjct: 86 ISFTWRNQRINLI 98
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 39.5 bits (88), Expect = 7e-04
Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 1/68 (1%)
Frame = +3
Query: 153 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 329
G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++
Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191
Query: 330 IKSTAISM 353
IKST +++
Sbjct: 192 IKSTPLTL 199
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 36.3 bits (80), Expect = 0.006
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = +3
Query: 165 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 344
KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S
Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108
Query: 345 I 347
+
Sbjct: 109 V 109
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 35.9 bits (79), Expect = 0.008
Identities = 27/85 (31%), Positives = 42/85 (49%), Gaps = 7/85 (8%)
Frame = +3
Query: 129 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAR--AGETRF-- 290
N + E DKKR IRN+ + AH+D GK+T T+ ++ G I G+
Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100
Query: 291 -TDTRKDEQDRCITIKSTAISMFFE 362
D + E+++ ITI+S A +E
Sbjct: 101 KMDFMELEREKGITIQSAATHCTWE 125
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.033
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 159 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 254
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.033
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 159 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 254
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 33.9 bits (74), Expect = 0.033
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +3
Query: 159 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 254
M K++ N+ VI HVD GKST T L+ K G
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.13
Identities = 17/52 (32%), Positives = 17/52 (32%)
Frame = +2
Query: 761 PPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXPXPXSXPXWGKXPPPPP 916
PPP P P P P P P P P G PPPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMG--GPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 2.1
Identities = 19/71 (26%), Positives = 21/71 (29%)
Frame = +1
Query: 700 PLSPSTYNXDXWXXXLTSXXTPPXAPXVXAPXPXXXXXPXPPPXXXXXXXQXPXXXXXXL 879
PL P + L PP P V P P P PPP P
Sbjct: 712 PLLPDVSDTVEEQQKLLLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP----- 766
Query: 880 XPXLGXXXPPP 912
P + PPP
Sbjct: 767 PPGVAGAGPPP 777
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 29.9 bits (64), Expect = 0.53
Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
Frame = +3
Query: 186 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 353
++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M
Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231
Query: 354 FFELEEK 374
FE K
Sbjct: 232 AFEAMRK 238
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.3 bits (60), Expect = 1.6
Identities = 20/74 (27%), Positives = 21/74 (28%), Gaps = 4/74 (5%)
Frame = +2
Query: 707 PHPPIMXTXGPXX*RPXPPPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXP----XP 874
P P P P PP P+P P P P P P P
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Query: 875 XSXPXWGKXPPPPP 916
P G P PPP
Sbjct: 1200 VPKPSVGVPPVPPP 1213
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 2.1
Identities = 17/56 (30%), Positives = 17/56 (30%), Gaps = 3/56 (5%)
Frame = +2
Query: 758 PPPPXPRPXRXXX---PXXGXXPPXPXXPXXXXXNXPXXPXPXSXPXWGKXPPPPP 916
PPPP PR P G P P P P S P PPP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 27.1 bits (57), Expect = 3.7
Identities = 16/56 (28%), Positives = 17/56 (30%)
Frame = +2
Query: 749 RPXPPPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXPXPXSXPXWGKXPPPPP 916
+P PPP R PP P P P P PPPPP
Sbjct: 289 KPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKP--PIGNGSSNSSLPPPPPP 342
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/75 (28%), Positives = 23/75 (30%), Gaps = 7/75 (9%)
Frame = +2
Query: 713 PPIMXTXGPXX*RPXPPPPXPRPXRXXXPXXG-----XXPPXPXXPXXXXXNXPXXP--X 871
PP + + P PPP P P G PP P P P P
Sbjct: 376 PPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSA 435
Query: 872 PXSXPXWGKXPPPPP 916
P S P PP P
Sbjct: 436 PPSLPMGAPAAPPLP 450
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/70 (24%), Positives = 19/70 (27%)
Frame = +2
Query: 707 PHPPIMXTXGPXX*RPXPPPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXPXPXSXP 886
P PP + P P PP P P P P P P P + P
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
Query: 887 XWGKXPPPPP 916
P P
Sbjct: 478 PAPAPAPAAP 487
Score = 25.8 bits (54), Expect = 8.6
Identities = 19/63 (30%), Positives = 20/63 (31%), Gaps = 11/63 (17%)
Frame = +2
Query: 761 PPPXPRPXRXXXPXXGXXPPXP-----XXPXXXXXNXPXXPXPXSXPXWG------KXPP 907
PPP P P PP P P + P P P S K PP
Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPP 312
Query: 908 PPP 916
PPP
Sbjct: 313 PPP 315
Score = 25.8 bits (54), Expect = 8.6
Identities = 20/58 (34%), Positives = 20/58 (34%), Gaps = 4/58 (6%)
Frame = +2
Query: 707 PHPPIMXTXG----PXX*RPXPPPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXP 868
P PP G P R PPPP PR P G PP P N P P
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPPPR----SAPSTGRQPP-PLSSSRAVSNPPAPP 392
>SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 577
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 485 LLLQKRNQRVPGESIKLIKKPFSLFSRWFRV 393
L L +N V G + +P SLF RWF++
Sbjct: 234 LYLSNKNAAVDGCPDDNLDEPGSLFMRWFKI 264
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = +2
Query: 758 PPPPXPRPXRXXXPXXGXXPPXPXXPXXXXXNXPXXPXP 874
PPPP P P PP P + P P P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 26.2 bits (55), Expect = 6.5
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 183 NMSVIAHVDHGKSTLTDSLVSKAGII 260
N+ I HVD GKSTL +++ G++
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,229,456
Number of Sequences: 5004
Number of extensions: 63474
Number of successful extensions: 231
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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