Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_J09
(942 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 163 2e-41
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 160 3e-40
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 33 0.044
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 33 0.058
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.10
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 29 0.72
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 28 1.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.2
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 28 2.2
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 23 2.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.9
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 6.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 8.8
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 8.8
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 8.8
>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 163 bits (397), Expect = 2e-41
Identities = 75/108 (69%), Positives = 90/108 (83%)
Frame = +1
Query: 160 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRK 339
AP +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRK
Sbjct: 8 APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRK 67
Query: 340 SEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 483
SE+EYYA+L++ VHHYSGNNI+LGTACGK +RV LA+ D GDSDI+
Sbjct: 68 SELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115
>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 109
Score = 160 bits (388), Expect = 3e-40
Identities = 73/104 (70%), Positives = 87/104 (83%)
Frame = +1
Query: 172 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 351
V KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63
Query: 352 YYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 483
YYA+L+K VHHY+G NI+LGTACGK +RV LAITD GDSDI+
Sbjct: 64 YYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 33.5 bits (73), Expect = 0.044
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +2
Query: 866 PPXPPXPXXSXPXXXXPPPPPP 931
PP PP P P PPPPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
Score = 32.7 bits (71), Expect = 0.077
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +2
Query: 851 PXXXXPPXPPXPXXSXPXXXXPPPPP 928
P PP PP P P PPPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.5 bits (58), Expect = 2.9
Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 2/26 (7%)
Frame = +2
Query: 866 PPXPPXPXXSXPXXXXP--PPPPPXP 937
PP P P P P PPPPP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 822 PPXXXPPPXDXXXPXPPXPPXP 887
PP PPP PP PP P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 33.1 bits (72), Expect = 0.058
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +2
Query: 851 PXXXXPPXPPXPXXSXPXXXXPPPPPPXP 937
P PP P S P PPPPPP P
Sbjct: 168 PPSFQPPSAAAPATSLPSDYNPPPPPPPP 196
Score = 26.2 bits (55), Expect(2) = 0.058
Identities = 11/24 (45%), Positives = 12/24 (50%), Gaps = 2/24 (8%)
Frame = +2
Query: 866 PPXPPX--PXXSXPXXXXPPPPPP 931
P PP P + P PPPPPP
Sbjct: 221 PEIPPTYTPKQADPLPAPPPPPPP 244
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +3
Query: 801 RAVXXXPPPXXXPPPXDXXXPXPPXPPXP 887
RAV PP P D P PP PP P
Sbjct: 217 RAVSPEIPPTYTPKQADPL-PAPPPPPPP 244
Score = 25.4 bits (53), Expect(2) = 0.058
Identities = 12/35 (34%), Positives = 13/35 (37%)
Frame = +2
Query: 782 PXXXXSPGRLAXPPXXXXAASXXPXXXXPPXPPXP 886
P SP PP A+ P PP PP P
Sbjct: 161 PNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPPP 195
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.3 bits (70), Expect = 0.10
Identities = 14/37 (37%), Positives = 15/37 (40%)
Frame = +3
Query: 777 ASXXVXGPRAVXXXPPPXXXPPPXDXXXPXPPXPPXP 887
A V P + PPP PP P PP PP P
Sbjct: 747 APIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 31.5 bits (68), Expect = 0.18
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +2
Query: 866 PPXPPXPXXSXPXXXXPPPPPP 931
PP PP P + PPPPPP
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPP 783
Score = 31.1 bits (67), Expect = 0.24
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = +2
Query: 851 PXXXXPPXPPXPXXSXPXXXXPPPPPP 931
P PP PP P PPPPPP
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 30.7 bits (66), Expect = 0.31
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 869 PXPPXPXXSXPXXXXPPPPPPXP 937
P PP P PPPPPP P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.5 bits (58), Expect = 2.9
Identities = 16/43 (37%), Positives = 16/43 (37%)
Frame = +2
Query: 809 LAXPPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPPXP 937
L PP A P P P P P PPPPPP P
Sbjct: 729 LKSPPPPPPAV-IVPTPAPAPIPVPPPA--PIMGGPPPPPPPP 768
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +3
Query: 819 PPPXXXPPPXDXXXPXPPXPPXP 887
P P PPP PP PP P
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPP 768
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 29.5 bits (63), Expect = 0.72
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +1
Query: 184 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 309
++KK I+ ++L SGKYC G T+ L++G + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 28.3 bits (60), Expect = 1.7
Identities = 14/38 (36%), Positives = 14/38 (36%)
Frame = +2
Query: 818 PPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPP 931
P AAS P P S P PPPPPP
Sbjct: 1064 PSTVTSAASSPSNISKPSAPVANNVSKPSAVPPPPPPP 1101
Score = 27.1 bits (57), Expect = 3.8
Identities = 12/39 (30%), Positives = 14/39 (35%)
Frame = +2
Query: 821 PXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPPXP 937
P +A+ P P P PPPPPP P
Sbjct: 1064 PSTVTSAASSPSNISKPSAPVANNVSKPSAVPPPPPPPP 1102
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = +2
Query: 809 LAXPPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPPXP 937
++ PP +A+ P P PP P S P PP P P
Sbjct: 1687 VSTPPVRPQSAA--PPQMSAPTPPPPPMSVPPPPSAPPMPAGP 1727
Score = 27.9 bits (59), Expect = 2.2
Identities = 13/40 (32%), Positives = 13/40 (32%)
Frame = +2
Query: 812 AXPPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPP 931
A PP P PP P P PPPP P
Sbjct: 1697 AAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 27.9 bits (59), Expect = 2.2
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +1
Query: 292 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 426
++ +VI++K P+RK I+ + L+ GVH ++ EL CG
Sbjct: 87 RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.4 bits (48), Expect(2) = 2.7
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +2
Query: 914 PPPPPPXP 937
PPPPPP P
Sbjct: 946 PPPPPPPP 953
Score = 22.2 bits (45), Expect(2) = 2.7
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +2
Query: 899 PXXXXPPPPPP 931
P PPPPPP
Sbjct: 942 PAFPPPPPPPP 952
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.9
Identities = 15/42 (35%), Positives = 15/42 (35%), Gaps = 2/42 (4%)
Frame = +2
Query: 818 PPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPP--PPPXP 937
PP AA P PP P S PPP PP P
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/48 (27%), Positives = 16/48 (33%)
Frame = +2
Query: 797 SPGRLAXPPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPPPPPPXPR 940
+P P AA P P P P + P P PP P+
Sbjct: 1116 APSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPK 1163
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 26.2 bits (55), Expect = 6.7
Identities = 17/58 (29%), Positives = 32/58 (55%)
Frame = +1
Query: 160 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 333
APK + KK KT++ + + ++ G K+ +K +R+G+ LVI+A + P+
Sbjct: 33 APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/41 (31%), Positives = 13/41 (31%), Gaps = 1/41 (2%)
Frame = +2
Query: 818 PPXXXXAASXXPXXXXPPXPPXPXXSXPXXXXPP-PPPPXP 937
PP P PP P P P PP P P P
Sbjct: 107 PPLPNEPVPEEPLPGEPPLPDEPVPEEPLPGEPPLPNEPVP 147
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 8.8
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = +2
Query: 869 PXPPXPXXSXPXXXXPPPPP 928
P PP P + PPPPP
Sbjct: 729 PAPPTPAPTPAVKHHPPPPP 748
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.8
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +2
Query: 851 PXXXXPPXPPXPXXSXPXXXXPPPPPPXP 937
P P PP P S PP PP P
Sbjct: 246 PSLSAPAPPPIPPPSNGTVSSPPNSPPRP 274
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,881,684
Number of Sequences: 5004
Number of extensions: 50983
Number of successful extensions: 266
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 479324640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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