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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_J06
         (983 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_6444| Best HMM Match : CRAM_rpt (HMM E-Value=3e-11)                 30   2.5  
SB_36998| Best HMM Match : TUDOR (HMM E-Value=0)                       29   4.4  
SB_6115| Best HMM Match : efhand (HMM E-Value=0.22)                    29   4.4  
SB_37335| Best HMM Match : Sigma54_activat (HMM E-Value=7.8)           29   7.6  

>SB_6444| Best HMM Match : CRAM_rpt (HMM E-Value=3e-11)
          Length = 2297

 Score = 30.3 bits (65), Expect = 2.5
 Identities = 40/175 (22%), Positives = 67/175 (38%), Gaps = 1/175 (0%)
 Frame = +1

Query: 184  QAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRHKLSAASAGLALDNVNGHGL 363
            ++G   V      G  ++  +   D NV   I + DFN +  +    A     +++  G 
Sbjct: 589  KSGDIDVEGPELKGPKVEGDIDTPDVNVKGDIDTPDFNVKGDIEGPDA-----DIDSPGW 643

Query: 364  SLTGTRIPGF-GEQLGVAGKVNLFHNNNHDLSAKAFAIRNSPSAIPNAPNFNTLGGGVDY 540
               G ++P F G   G  GK     + + D+    F   N    I + P+ N L G +D 
Sbjct: 644  KFKGPKMPKFKGPSFGGKGK-----SGDIDIEGPDFDGPNVKGDI-DTPDIN-LKGDIDT 696

Query: 541  MFKQKVGASLSAAHSDVINRNDYSAGGKLNLFRSPSSSLDFNAGFKKFDTPFYRS 705
                 V   +    +D+        G K+  F+ PS      +G   FD P ++S
Sbjct: 697  P-DVNVKGDIDGPDADLHGPGWKFKGPKMPKFKGPSFGGKGKSGDIDFDGPDFKS 750



 Score = 29.9 bits (64), Expect = 3.3
 Identities = 40/171 (23%), Positives = 64/171 (37%), Gaps = 1/171 (0%)
 Frame = +1

Query: 184 QAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRHKLSAASAGLALDNVNGHGL 363
           ++G   V      G  ++  +   D NV   I + DFN +  +    A     ++NG   
Sbjct: 181 KSGDIDVEGPELKGPKVEGDIDTPDVNVKGDIDTPDFNVKGDIDGPDA-----DINGPSW 235

Query: 364 SLTGTRIPGF-GEQLGVAGKVNLFHNNNHDLSAKAFAIRNSPSAIPNAPNFNTLGGGVDY 540
              G ++P F G   G  GK     + + D+    F   N    I + P+ N L G +D 
Sbjct: 236 KFKGPKMPKFKGPSFGGKGK-----SGDIDIEGPDFDGPNVKGDI-DTPDIN-LKGDIDT 288

Query: 541 MFKQKVGASLSAAHSDVINRNDYSAGGKLNLFRSPSSSLDFNAGFKKFDTP 693
                V   +    +DV        G K+  F+ PS      +G   +D P
Sbjct: 289 P-DVNVKGDIDGPDADVHGPGWKFKGPKMPKFKGPSFGGKGKSGDIDYDGP 338


>SB_36998| Best HMM Match : TUDOR (HMM E-Value=0)
          Length = 2538

 Score = 29.5 bits (63), Expect = 4.4
 Identities = 18/50 (36%), Positives = 26/50 (52%)
 Frame = -1

Query: 506 GALGMALGEFLIANALALRSWLLLWNKLTLPATPSCSPKPGMRVPVRLSP 357
           G + M + + LIA   A+++     N L +PA  S  PKP   V V+ SP
Sbjct: 395 GDMDMQISQILIAAGYAVKTSEFNPNPLRIPAAKSTPPKPA-PVQVQASP 443


>SB_6115| Best HMM Match : efhand (HMM E-Value=0.22)
          Length = 822

 Score = 29.5 bits (63), Expect = 4.4
 Identities = 17/53 (32%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
 Frame = +1

Query: 160 HVPTRARRQAGSFTVNS---DGTSGAALKVPLTGNDKNVLSAIGSADFNDRHK 309
           HVP +     G +  NS     T G+ + +PL  ++K V+  +G    ND HK
Sbjct: 526 HVP-KVGSHGGVYFWNSFRNKDTDGSLIVLPLKDHEKRVIGLLGVDTLNDSHK 577


>SB_37335| Best HMM Match : Sigma54_activat (HMM E-Value=7.8)
          Length = 206

 Score = 28.7 bits (61), Expect = 7.6
 Identities = 20/50 (40%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
 Frame = -1

Query: 416 PATPSCSP--KPGMRVPVRLSPCPFTLSRASPA-EAALSLWRSLKSADPM 276
           P TPSCSP  +  + +PVRL      LS  +P  EA + L  S+K+  P+
Sbjct: 10  PLTPSCSPLERDVLALPVRLG----GLSMTNPCLEADIELSSSVKATAPL 55


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,685,469
Number of Sequences: 59808
Number of extensions: 412559
Number of successful extensions: 1162
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1032
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1147
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2919714245
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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