Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_I18
(961 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 222 7e-59
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 126 6e-30
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 124 2e-29
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 119 5e-28
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 116 4e-27
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 114 2e-26
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 94 2e-20
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 87 3e-18
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 50 6e-07
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 3.9
SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter |Schizo... 27 5.2
SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 27 5.2
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 6.9
SPAC23D3.12 |||inorganic phosphate transporter |Schizosaccharomy... 26 9.1
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 222 bits (542), Expect = 7e-59
Identities = 125/242 (51%), Positives = 161/242 (66%), Gaps = 2/242 (0%)
Frame = +2
Query: 143 LSVAGTRSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKM 322
L ++G + G+ VR QNV+ N+VKSSLGPVGLDKMLVDDIGDVTVTNDGATIL +
Sbjct: 10 LFLSGEKISGEDVRNQNVLATTAIANVVKSSLGPVGLDKMLVDDIGDVTVTNDGATILSL 69
Query: 323 LEVEHPAAKVLVELAQLQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLX 502
L+VEHPA KVLVELAQ QD+EVGDGTTSVVIIAAELL+ A+ELVK KIHPT+II+GYRL
Sbjct: 70 LDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLA 129
Query: 503 CKEAVKYIQDNLTVTVESLGQ--AVSDQHSQNXYVV*TNWS*CRFLLXXXXXXXXXXQNN 676
+EAVK++ D L+ +V+SLG+ ++ + + N S + N+
Sbjct: 130 IREAVKFMTDVLSCSVDSLGKESLINVAKTSMSSKIIGNDSDFFSTMAVDAMLSVKTSNS 189
Query: 677 EPQRKHYIPYQSY*HSESPRXSAXXSVLVKGYALNCTVPSQACPXTFSXAKIPCPDFSLP 856
+ + ++ P ++ ++ S+ SVLVKGYALNCT+ SQA AKI D L
Sbjct: 190 KGETRY--PVKAVNILKAHGKSSRESVLVKGYALNCTIASQAMKTRVQNAKIAVLDMDLQ 247
Query: 857 XT 862
T
Sbjct: 248 KT 249
Score = 62.1 bits (144), Expect = 1e-10
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = +3
Query: 558 LXRPSLINTAKTTMSSKLIGADADFFSEMVVXAAQAIKTMNPRGNTIYP 704
L + SLIN AKT+MSSK+IG D+DFFS M V A ++KT N +G T YP
Sbjct: 148 LGKESLINVAKTSMSSKIIGNDSDFFSTMAVDAMLSVKTSNSKGETRYP 196
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 126 bits (303), Expect = 6e-30
Identities = 59/118 (50%), Positives = 85/118 (72%)
Frame = +2
Query: 173 DPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKV 352
D V++ +++ NIV++SLGP GLDK+L+ G++TVTNDGATIL +EVEH AK+
Sbjct: 34 DAVKS-HILATKTVANIVRTSLGPRGLDKILISPDGEITVTNDGATILDQMEVEHQIAKL 92
Query: 353 LVELAQLQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYI 526
LV+L++ QD+E+GDGTT VV++A LL+ A+ L+ IHP I GY C+ AVK++
Sbjct: 93 LVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGYEKACQVAVKHL 150
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 124 bits (298), Expect = 2e-29
Identities = 60/123 (48%), Positives = 87/123 (70%), Gaps = 1/123 (0%)
Frame = +2
Query: 152 AGTRSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDI-GDVTVTNDGATILKMLE 328
+G + G+ R + + ++VKS+LGP G+DK+L + GD+ VTNDGATILK +
Sbjct: 12 SGIQERGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQSNSSGDIVVTNDGATILKSIA 71
Query: 329 VEHPAAKVLVELAQLQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCK 508
+++ AAKVLV ++++QD+EVGDGTTSV + AAELL+ A+ +V KIHP II GYR+ K
Sbjct: 72 LDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATK 131
Query: 509 EAV 517
A+
Sbjct: 132 TAI 134
Score = 30.7 bits (66), Expect = 0.32
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +3
Query: 564 RPSLINTAKTTMSSKLIGADADFFSEMVVXAAQAIK 671
R L N A+TT+SSK++ + + F+++ V A +K
Sbjct: 152 RSDLENIARTTLSSKILSQNKNHFAQLAVDAVLRLK 187
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 119 bits (287), Expect = 5e-28
Identities = 57/136 (41%), Positives = 87/136 (63%)
Frame = +2
Query: 143 LSVAGTRSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKM 322
++ G R G + N+ +++++ LGP + KML+D +G V +TNDG IL+
Sbjct: 8 MNTNGNRQVGHKAQMSNIQAAKAVADVIRTCLGPRAMLKMLLDPVGSVLLTNDGHAILRE 67
Query: 323 LEVEHPAAKVLVELAQLQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLX 502
+EV HPAAK ++ELA+ QDEEVGDGTTSV+I+A E+L A L+ KIHP +I ++
Sbjct: 68 IEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQA 127
Query: 503 CKEAVKYIQDNLTVTV 550
++A+ I D +T+ V
Sbjct: 128 LEDALSII-DEITLPV 142
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 116 bits (280), Expect = 4e-27
Identities = 54/114 (47%), Positives = 80/114 (70%)
Frame = +2
Query: 191 NVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQ 370
N+ + ++++LGP+G DK++VDD G+V ++NDGATI+K+L++ HPAAK LV++A+
Sbjct: 29 NINACVAVQDTIRTTLGPLGADKLMVDDRGEVVISNDGATIMKLLDIVHPAAKTLVDIAR 88
Query: 371 LQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYIQD 532
QD EVGDGTTSVV+ A ELL+ A V+ + II GYR + AV I++
Sbjct: 89 AQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLAVNKIKE 142
Score = 31.1 bits (67), Expect = 0.24
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +3
Query: 564 RPSLINTAKTTMSSKLIGADADFFSEMVVXAAQAIKTMNPRGNTI 698
R L A T M+SKLI +++ FF++MVV A + + N I
Sbjct: 156 RDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTLDQEDLNENMI 200
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 114 bits (274), Expect = 2e-26
Identities = 57/119 (47%), Positives = 79/119 (66%)
Frame = +2
Query: 179 VRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLV 358
VR N+M + +++SLGP G+DKM+ G+V +TNDGATILK L V HPAAK+LV
Sbjct: 20 VRLSNIMAARSVADAIRTSLGPKGMDKMIQTGKGEVILTNDGATILKHLSVLHPAAKMLV 79
Query: 359 ELAQLQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYIQDN 535
+L+ QD E GDGTTSVVI+A +L A++L+K IHPT I ++ V +++N
Sbjct: 80 DLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTVDCMKEN 138
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 94.3 bits (224), Expect = 2e-20
Identities = 41/112 (36%), Positives = 68/112 (60%)
Frame = +2
Query: 191 NVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQ 370
N+ +++KS+LGP G KMLVD G + +T DG +L +++++P A + + A
Sbjct: 21 NISAAIGLQDVLKSNLGPTGTTKMLVDGAGAIKLTKDGKVLLTEMQIQNPTASCIAKAAT 80
Query: 371 LQDEEVGDGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYI 526
QD+ GDGTTSV ++ ELLK A+ ++ +HP+ I G+ L EA+ ++
Sbjct: 81 AQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGFNLAKNEALTFL 132
>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 546
Score = 87.4 bits (207), Expect = 3e-18
Identities = 39/113 (34%), Positives = 69/113 (61%)
Frame = +2
Query: 221 IVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQLQDEEVGDGT 400
I ++SLGP G +K++V+ + +TND ATI++ LEV HPAAK++V+ Q Q+ E+GD
Sbjct: 41 ITRTSLGPNGKNKIVVNHLQQTFLTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAA 100
Query: 401 TSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYIQDNLTVTVESL 559
VV+ ELL A+ +++ + P I GY + ++ +++ +E++
Sbjct: 101 NFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHTMEVLEEICADKIETV 153
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 49.6 bits (113), Expect = 6e-07
Identities = 26/108 (24%), Positives = 52/108 (48%), Gaps = 4/108 (3%)
Frame = +2
Query: 224 VKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEV----EHPAAKVLVELAQLQDEEVG 391
V +LGP G + ++ G +T DG T+ + + + E+ A+++ ++A +E G
Sbjct: 58 VSVTLGPKGRNVLIDQPFGSPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAG 117
Query: 392 DGTTSVVIIAAELLKNADELVKTKIHPTSIISGYRLXCKEAVKYIQDN 535
DGTT+ ++ + V +P + G +L V+++Q N
Sbjct: 118 DGTTTATVLTRAIFSETVRNVAAGCNPMDLRRGIQLAVDNVVEFLQAN 165
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.1 bits (57), Expect = 3.9
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = +2
Query: 401 TSVVIIAAELLKNADELVKTKIHPTSIISGY 493
T++++++ LL E + TK+HP+ +S Y
Sbjct: 302 TNLIVVSVALLHQWAEELSTKVHPSKKLSVY 332
>SPBC8E4.01c ||SPBP4G3.01|inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 572
Score = 26.6 bits (56), Expect = 5.2
Identities = 7/18 (38%), Positives = 14/18 (77%)
Frame = +3
Query: 231 APLGQLVWIRCWLTILGM 284
+PL +++W+ CW +LG+
Sbjct: 149 SPLSKMMWVFCWRWLLGV 166
>SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 573
Score = 26.6 bits (56), Expect = 5.2
Identities = 7/18 (38%), Positives = 14/18 (77%)
Frame = +3
Query: 231 APLGQLVWIRCWLTILGM 284
+PL +++W+ CW +LG+
Sbjct: 149 SPLSKMMWVFCWRWLLGV 166
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 26.2 bits (55), Expect = 6.9
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = -2
Query: 159 VPATDKGAAIVDIFYCIFNTKFTLKSPSRFFCFWKELQILRNSA 28
VP+ + ++D+F+ IF +++ S S F K L +++N A
Sbjct: 495 VPSFAPRSDVIDLFFLIFQVEYS--SWSESFLAGKRLTVVKNQA 536
>SPAC23D3.12 |||inorganic phosphate transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 559
Score = 25.8 bits (54), Expect = 9.1
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3
Query: 234 PLGQLVWIRCWLTILGM 284
PLG+++WI W +LG+
Sbjct: 145 PLGKMMWIFAWRWLLGL 161
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,121,447
Number of Sequences: 5004
Number of extensions: 57197
Number of successful extensions: 138
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 491307756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -