Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_I16
(925 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 31 0.17
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.93
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 29 1.2
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 27 4.9
SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa... 27 4.9
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.5
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 6.5
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 26 8.6
SPBC1271.13 |mrpl8||mitochondrial ribosomal protein subunit L8|S... 26 8.6
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 31.5 bits (68), Expect = 0.17
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = +3
Query: 135 AGVVELSADSMSPSNQDLEDKLYNSILTGDYDSAVRKSLE 254
A +V+ S +P + DLE + ++LTGD SAV+ LE
Sbjct: 537 AEIVKDSFKIFNPEDSDLEKNITEALLTGDVLSAVKACLE 576
>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 300
Score = 29.1 bits (62), Expect = 0.93
Identities = 14/26 (53%), Positives = 18/26 (69%)
Frame = -3
Query: 254 FQALTYSTVVVTGEDAVVQFVLEVLV 177
F +T V+V EDAVV+FVL +LV
Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206
>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
termination factor Reb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 504
Score = 28.7 bits (61), Expect = 1.2
Identities = 11/59 (18%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +1
Query: 277 SIVQNVVNNLIIDKRRNTMEYCYKLWVGNGQDIVKKYFPLSFR--THHGRKLRQAHLQK 447
+I+ V+N I+D+ + ++C ++W G ++ ++ ++ +H K H+++
Sbjct: 247 AIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRR 305
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +3
Query: 120 VLAASAGVVELSADSMSPSNQDLEDKLYNSILTGDYDSAVRKSLEYESQGQG 275
V+ A+AG+ + S+ N+D+ K+ L G Y +A ++ QG+G
Sbjct: 91 VMIANAGIA-IPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKG 141
>SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 889
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 280 IVQNVVNNLIIDKRRNTMEYCYKLWVGNGQDIVK 381
+++++ NL I N +EY LW NG I K
Sbjct: 447 VLKDIFFNLQIGVTFNILEYLRHLWSNNGDAIAK 480
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.5
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -1
Query: 922 GGXXGTNGGXXXGXRGGXRG 863
GG G GG G RGG RG
Sbjct: 26 GGFGGGRGGARGGGRGGARG 45
Score = 25.8 bits (54), Expect = 8.6
Identities = 20/56 (35%), Positives = 20/56 (35%), Gaps = 2/56 (3%)
Frame = -1
Query: 922 GGXXGTNGGXXX--GXRGGXRGMXVAPKR*RRSKXXGRERXXXGXXXXGXDAVGGA 761
GG G NGG G RGG RG R R G G A GGA
Sbjct: 16 GGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGGA 71
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 124 SPPAPASWNYPRTA*ALLTKTSRTNCTTASS 216
+PP PAS + T+ + L TS T+CTT++S
Sbjct: 315 TPPPPASTSSTGTSSSPLPSTS-TSCTTSTS 344
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 124 SPPAPASWNYPRTA*ALLTKTSRTNCTTASS 216
+PP PAS + T+ + L TS T+CTT++S
Sbjct: 369 TPPPPASTSSTGTSSSPLLSTS-TSCTTSTS 398
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 5/40 (12%)
Frame = -2
Query: 393 WEVLFDNILS--VADPQL---VAVLHGVPSLVNDQIVNYI 289
W+VLF + L+ ++ P V LHGV + VN + +YI
Sbjct: 188 WDVLFHDYLNETLSQPAFSFNVPDLHGVDNKVNQYVFDYI 227
>SPBC1271.13 |mrpl8||mitochondrial ribosomal protein subunit
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 207
Score = 25.8 bits (54), Expect = 8.6
Identities = 29/100 (29%), Positives = 42/100 (42%), Gaps = 4/100 (4%)
Frame = +1
Query: 175 LTKTSRTNCTTASSPVTTTVLYVRAWNTRAKARXSIVQNVVNNLI-IDKRRNTMEYCYKL 351
L +TS + VT+ V + T AKA+ + Q LI + KR N L
Sbjct: 10 LGRTSAHRQALLRNLVTSLVKHESIQTTWAKAKEA--QRFAEKLITMAKRANPQNNRKGL 67
Query: 352 WVG---NGQDIVKKYFPLSFRTHHGRKLRQAHLQKLQPRS 462
G + +KK F + ++GR+ L KL PRS
Sbjct: 68 AEGMVFEKETTLKKVFDVLVPRYNGRRCGYTRLLKLPPRS 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,731,145
Number of Sequences: 5004
Number of extensions: 46160
Number of successful extensions: 145
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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