Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_I13
(991 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 52 2e-07
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 49 9e-07
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 49 1e-06
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 41 3e-04
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 37 0.004
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 36 0.007
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 31 0.33
SPCC18.17c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 28 1.8
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 28 2.3
SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces po... 27 5.4
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 26 7.1
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 51.6 bits (118), Expect = 2e-07
Identities = 29/70 (41%), Positives = 41/70 (58%)
Frame = +2
Query: 152 LSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGR 331
L +G IP + LGTW GK ++T+ AV AL+AGY HIDTA+ YG E ++G
Sbjct: 17 LPNGDKIPSIGLGTWRS----GK-----DETKNAVCAALKAGYRHIDTAHIYGNEKEIGE 67
Query: 332 ALXQEILRRT 361
+ + + RT
Sbjct: 68 GIRESGVPRT 77
Score = 44.8 bits (101), Expect = 2e-05
Identities = 36/119 (30%), Positives = 55/119 (46%), Gaps = 11/119 (9%)
Frame = +3
Query: 357 GLKRESIFITTKLWNSYHEREQVIPALRKSLXKSQLG------IRRPVF-----DSLAHS 503
G+ R I++T+KLW + H V AL K+L L I P + L +
Sbjct: 73 GVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPRN 132
Query: 504 YV*ERYLARHVDFLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQIE 680
E + V +TW+ M + + G + IGISNFN E + R++ KP+ Q+E
Sbjct: 133 EKGE-LIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQME 190
Score = 38.3 bits (85), Expect = 0.002
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +1
Query: 448 LXNLNLEYVDLYLIHWPIAM 507
L +LNLEY+D YLIHWP A+
Sbjct: 103 LQDLNLEYIDAYLIHWPFAL 122
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 49.2 bits (112), Expect = 9e-07
Identities = 34/108 (31%), Positives = 57/108 (52%), Gaps = 1/108 (0%)
Frame = +3
Query: 360 LKRESIFITTKLWNSYHEREQVIPALRKSLXKSQLGIRRPVFDS-LAHSYV*ERYLARHV 536
+KRE I + TK+ +S E+ A+ +SL ++ RP D L HS ++ R +
Sbjct: 67 IKREDITLITKIPDSLQGFERTWKAVEQSLRRTG----RPKLDVVLIHS---PKWPVRRI 119
Query: 537 DFLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQIE 680
+ +WR ++ +K G IG+SN+N ++ II GL P+ Q+E
Sbjct: 120 E---SWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVE 164
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 48.8 bits (111), Expect = 1e-06
Identities = 35/118 (29%), Positives = 58/118 (49%), Gaps = 10/118 (8%)
Frame = +3
Query: 357 GLKRESIFITTKLWNSYHEREQVIPALRKSLXKSQLG------IRRPV-FDSLAHSYV*E 515
G+ R+ I++T+KLW + H E V AL K+L +L I PV F + + +
Sbjct: 74 GVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPKD 133
Query: 516 R---YLARHVDFLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQIE 680
+ + +TW+ M + G + IG+SNFN ++RI+ KP+ Q+E
Sbjct: 134 KDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQME 191
Score = 45.2 bits (102), Expect = 1e-05
Identities = 26/64 (40%), Positives = 33/64 (51%)
Frame = +2
Query: 152 LSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGR 331
L+ G IP + LGTW N T+ AV AL+ GY HID A YG ED+VG
Sbjct: 18 LADGSKIPGLGLGTWRSE---------PNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGD 68
Query: 332 ALXQ 343
+ +
Sbjct: 69 GIKE 72
Score = 32.3 bits (70), Expect = 0.11
Identities = 11/19 (57%), Positives = 16/19 (84%)
Frame = +1
Query: 448 LXNLNLEYVDLYLIHWPIA 504
L +L L+Y+D YLIHWP++
Sbjct: 104 LKDLKLDYLDEYLIHWPVS 122
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 40.7 bits (91), Expect = 3e-04
Identities = 24/64 (37%), Positives = 37/64 (57%)
Frame = +2
Query: 170 IPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALXQEI 349
+P +GT L + KG++ + D+ V AL AG+ HID A YG E++VG AL +
Sbjct: 12 VPAYGVGTALFKKEKGEINRTIVDS---VKNALAAGFIHIDCAEVYGNEEEVGVALKEAN 68
Query: 350 LRRT 361
+ R+
Sbjct: 69 VPRS 72
Score = 38.7 bits (86), Expect = 0.001
Identities = 31/106 (29%), Positives = 50/106 (47%), Gaps = 1/106 (0%)
Frame = +3
Query: 366 RESIFITTKLWNSYHEREQVIPALRKSLXKSQLGIRRPVFDS-LAHSYV*ERYLARHVDF 542
R +FIT+K+ H + + AL +SL K LG D L HS + + + +
Sbjct: 71 RSKLFITSKVM---HNVDNIPEALNESLRK--LGT--DYLDLYLLHSPI--PFYEKKIPI 121
Query: 543 LDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQIE 680
+ W+ M A GL S+G+SNF ++ ++ + P QIE
Sbjct: 122 SEGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIE 167
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 37.1 bits (82), Expect = 0.004
Identities = 19/49 (38%), Positives = 27/49 (55%)
Frame = +2
Query: 236 NDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALXQEILRRT*KRIYLY 382
N+ V AL++GY HIDTA YG ED G+A+ + KR ++
Sbjct: 32 NECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCEKNNVKRTDIF 80
Score = 25.8 bits (54), Expect = 9.4
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +3
Query: 549 TWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLE-KPSALQIE 680
+W+ M + G +S+G+SN+ + +Q + S + P QIE
Sbjct: 127 SWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIE 171
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 36.3 bits (80), Expect = 0.007
Identities = 31/103 (30%), Positives = 51/103 (49%), Gaps = 2/103 (1%)
Frame = +3
Query: 345 KFSE--GLKRESIFITTKLWNSYHEREQVIPALRKSLXKSQLGIRRPVFDSLAHSYV*ER 518
KF E G KRE I+ T+KL N + + ++ S+ LG +F L HS
Sbjct: 62 KFMEETGTKREDIWFTSKL-NDLSGYKSTLSSIDASVKACGLGYI-DLF--LLHS----- 112
Query: 519 YLARHVDFLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDS 647
+ D +++W+ + + G ++IG+SNF IQ ++DS
Sbjct: 113 ---PYGDRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDS 152
Score = 31.9 bits (69), Expect = 0.14
Identities = 21/64 (32%), Positives = 33/64 (51%)
Frame = +2
Query: 146 LQLSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQV 325
++L++G P A G+++ +R K +V AL+ GY HID+A Y E
Sbjct: 6 VKLNNGLKCPQFAYGSYMVNRTK---------CFDSVYAALQCGYRHIDSAQMYHNEADC 56
Query: 326 GRAL 337
GRA+
Sbjct: 57 GRAI 60
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 30.7 bits (66), Expect = 0.33
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = +3
Query: 540 FLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQI 677
++DTW+ + + G ++G+S F ++QR+I S P + QI
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQI 203
>SPCC18.17c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 488
Score = 28.3 bits (60), Expect = 1.8
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
Frame = +2
Query: 233 TNDTEQAVIYALEAGYTHIDTAY--KYGIEDQVGRALXQEILRR 358
TND ++Y + T + A KYGIED+ R + + + RR
Sbjct: 305 TNDATSILLYGVFLSNTVVTQALIKKYGIEDEANRYVPRWLTRR 348
>SPBC19G7.10c |||topoisomerase associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 744
Score = 27.9 bits (59), Expect = 2.3
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +3
Query: 540 FLDTWRGMIDAKKLGLTKSIGISNFNQEQIQRIIDSGLEKPSALQI 677
F+ T+ G + ++ G + Q+Q+QR++ S E+P A Q+
Sbjct: 364 FIQTYLGPSNNRRRGRRSENPMQKL-QQQLQRLVSSAKERPKATQL 408
>SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 418
Score = 26.6 bits (56), Expect = 5.4
Identities = 28/87 (32%), Positives = 37/87 (42%), Gaps = 5/87 (5%)
Frame = +2
Query: 236 NDTEQAVIYA---LEAGYTHIDTAYKYGIEDQVGRALXQEILRR--T*KRIYLYNYQALE 400
NDT A+ A LEAG THIDT+ GI ++ G +L R R Y+ + L
Sbjct: 227 NDTGMAIANAYCALEAGATHIDTSI-LGIGERNGITPLGALLARMYVTDREYITHKYKLN 285
Query: 401 FLPRERASDPSAEEVPXKISTWNTSTC 481
L A EV + + T C
Sbjct: 286 QLRELENLVADAVEVQIPFNNYITGMC 312
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 7.1
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 707 LFKFCRENDIXVMAYTPSG 763
+ K CREN+I ++ Y P G
Sbjct: 193 VMKACRENNITIVCYAPLG 211
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,462,495
Number of Sequences: 5004
Number of extensions: 72436
Number of successful extensions: 232
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 511279616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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