Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_I07
(921 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 148 1e-36
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 145 9e-36
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 95 9e-21
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 76 6e-15
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 68 2e-12
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 46 1e-05
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 36 0.008
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 28 1.6
SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 3.7
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 26 6.5
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.5
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 26 6.5
SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual 26 8.6
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 26 8.6
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 26 8.6
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 148 bits (358), Expect = 1e-36
Identities = 70/154 (45%), Positives = 94/154 (61%)
Frame = +1
Query: 130 TFNNGESIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKE 309
T +G IP GLGTW+S+P + AVK A+ GYRHID A +YGNE EVG+ I KE
Sbjct: 17 TLADGSKIPGLGLGTWRSEPNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGDGI----KE 72
Query: 310 GVVTREDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDLYLIHWPQAYKEDGSLFPT 489
V R+D+++TSKLW H P+ V L++TL +L + YLD YLIHWP ++K FP
Sbjct: 73 SGVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPK 132
Query: 490 XQSGKIQFSDXDYVDXWKAMEPLXKEGLVXSIGV 591
+ G + + + WKAME L + G V IG+
Sbjct: 133 DKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGL 166
Score = 39.5 bits (88), Expect = 7e-04
Identities = 19/41 (46%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Frame = +3
Query: 594 NFNSKQLERLLQXATVKPVVNQVECHP-LPHQKXLXSSVRL 713
NFN LER+L+ A VKP V+Q+E HP LP + + +L
Sbjct: 168 NFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKL 208
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 145 bits (351), Expect = 9e-36
Identities = 70/154 (45%), Positives = 94/154 (61%)
Frame = +1
Query: 130 TFNNGESIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKE 309
T NG+ IP GLGTW+S E AV A+ GYRHID A++YGNEKE+GE I +E
Sbjct: 16 TLPNGDKIPSIGLGTWRSGKDETKNAVCAALKAGYRHIDTAHIYGNEKEIGEGI----RE 71
Query: 310 GVVTREDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDLYLIHWPQAYKEDGSLFPT 489
V R D+++TSKLW HR LV L++TL +LN++Y+D YLIHWP A P
Sbjct: 72 SGVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPR 131
Query: 490 XQSGKIQFSDXDYVDXWKAMEPLXKEGLVXSIGV 591
+ G++ + D + W+AME L + G V IG+
Sbjct: 132 NEKGELIYEDVPIEETWQAMEELLETGKVRYIGI 165
Score = 39.5 bits (88), Expect = 7e-04
Identities = 18/41 (43%), Positives = 28/41 (68%), Gaps = 1/41 (2%)
Frame = +3
Query: 594 NFNSKQLERLLQXATVKPVVNQVECHP-LPHQKXLXSSVRL 713
NFN++ L+R+L+ A VKP ++Q+E HP LP + L +L
Sbjct: 167 NFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKKL 207
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 95.5 bits (227), Expect = 9e-21
Identities = 72/172 (41%), Positives = 91/172 (52%), Gaps = 6/172 (3%)
Frame = +1
Query: 94 LINKMAPKIPVVTFNNGESIPVFGLGT--WKSKPGEVTQ----AVKDAIDIGYRHIDCAY 255
LI M PKIPV P +G+GT +K + GE+ + +VK+A+ G+ HIDCA
Sbjct: 2 LIAAMGPKIPV---------PAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAE 52
Query: 256 VYGNEKEVGEAITSKIKEGVVTREDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDL 435
VYGNE+EVG A +KE V R LFITSK+ H D + L E+L L YLDL
Sbjct: 53 VYGNEEEVGVA----LKEANVPRSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDL 105
Query: 436 YLIHWPQAYKEDGSLFPTXQSGKIQFSDXDYVDXWKAMEPLXKEGLVXSIGV 591
YL+H P + E KI S + WKAME GLV S+GV
Sbjct: 106 YLLHSPIPFYEK----------KIPIS-----EGWKAMETALGTGLVHSVGV 142
Score = 34.3 bits (75), Expect = 0.025
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +3
Query: 567 GSGXEHRS--PNFNSKQLERLLQXATVKPVVNQVECHP 674
G+G H NF LE LL+ +T+ P VNQ+E HP
Sbjct: 133 GTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHP 170
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 76.2 bits (179), Expect = 6e-15
Identities = 55/155 (35%), Positives = 72/155 (46%)
Frame = +1
Query: 127 VTFNNGESIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIK 306
VT NG IP G G + K E V A+D GYRHID A VYGNE G+AI +
Sbjct: 11 VTLTNGMVIPRIGFGAFMLKYNECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCE 70
Query: 307 EGVVTREDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDLYLIHWPQAYKEDGSLFP 486
+ V R D+F+TSKL N ++ +L +L Y+DL+LI P K+
Sbjct: 71 KNNVKRTDIFLTSKLANCSDYYS-TRAAIRSSLHHLGT-YIDLFLIQSPAGGKK------ 122
Query: 487 TXQSGKIQFSDXDYVDXWKAMEPLXKEGLVXSIGV 591
+ WKAME G + S+GV
Sbjct: 123 ------------SRIASWKAMEEFVDSGDIRSVGV 145
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 68.1 bits (159), Expect = 2e-12
Identities = 47/155 (30%), Positives = 71/155 (45%)
Frame = +1
Query: 127 VTFNNGESIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIK 306
V NNG P F G++ + +V A+ GYRHID A +Y NE + G AI ++
Sbjct: 6 VKLNNGLKCPQFAYGSYMVNRTKCFDSVYAALQCGYRHIDSAQMYHNEADCGRAILKFME 65
Query: 307 EGVVTREDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDLYLIHWPQAYKEDGSLFP 486
E RED++ TSKL N + ++ + Y+DL+L+H P
Sbjct: 66 ETGTKREDIWFTSKL-NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSPYG--------- 115
Query: 487 TXQSGKIQFSDXDYVDXWKAMEPLXKEGLVXSIGV 591
D ++ WKA+E +EG + +IGV
Sbjct: 116 ------------DRIESWKALEKGVEEGKLRAIGV 138
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 45.6 bits (103), Expect = 1e-05
Identities = 27/86 (31%), Positives = 46/86 (53%), Gaps = 1/86 (1%)
Frame = +1
Query: 202 QAVKDAIDIGYRHIDCAYVYGNEKEVGEAITSKIKEGV-VTREDLFITSKLWNTFHRPDL 378
Q V +A+ +G R ID A Y NEKE +AI + V + RED+ + +K+ ++ +
Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFER 87
Query: 379 VXGXLQETLDNLNVQYLDLYLIHWPQ 456
++++L LD+ LIH P+
Sbjct: 88 TWKAVEQSLRRTGRPKLDVVLIHSPK 113
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 35.9 bits (79), Expect = 0.008
Identities = 35/120 (29%), Positives = 53/120 (44%), Gaps = 15/120 (12%)
Frame = +1
Query: 139 NGESIPVFGLGTWKSKPGE------VTQAVKDAIDIGYRHIDCAYVYGN---EKEVGEAI 291
+G + F LG W + E +K A D+G D A +Y N E +G+AI
Sbjct: 21 SGLKVSAFSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGNSETVMGKAI 80
Query: 292 TS---KIKEGVVTREDLF-ITSKLWNT--FHRPDLVXGXLQETLDNLNVQYLDLYLIHWP 453
E V+T + F +KL NT R ++ G L +L L + Y+D+ + H P
Sbjct: 81 KELGWDRSEYVITTKVFFGAGTKLPNTTGLSRKHIIEG-LNASLKRLGLPYVDVIMAHRP 139
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/43 (27%), Positives = 19/43 (44%)
Frame = +1
Query: 133 FNNGESIPVFGLGTWKSKPGEVTQAVKDAIDIGYRHIDCAYVY 261
FN+GE +P+F L W+ G + + G +D Y
Sbjct: 1979 FNHGEQLPLFILANWRGFSGGQRDMFNEVLKYGSYIVDALASY 2021
>SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 390
Score = 27.1 bits (57), Expect = 3.7
Identities = 10/39 (25%), Positives = 19/39 (48%)
Frame = +1
Query: 334 FITSKLWNTFHRPDLVXGXLQETLDNLNVQYLDLYLIHW 450
+ + + + R +L+ L E L + LD+Y +HW
Sbjct: 123 YFSKEYLTAYRRSELLQNKLHEYQKKLITRVLDMYQLHW 161
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 6.5
Identities = 7/23 (30%), Positives = 16/23 (69%)
Frame = +1
Query: 496 SGKIQFSDXDYVDXWKAMEPLXK 564
S +++F+ DY+ W+A+ P+ +
Sbjct: 162 SQRVEFARLDYIKVWRALAPIYR 184
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.5
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -2
Query: 794 GGRGXXKGGGXXRXFGXRGG 735
GGRG +GGG G RGG
Sbjct: 30 GGRGGARGGGRGGARGGRGG 49
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/75 (25%), Positives = 27/75 (36%)
Frame = +2
Query: 479 SSRPXNQVKSSSRTSITWTPGRLWSRSSKRVWSRASESQLQLEAAGEVAAARDCQAGRQS 658
SS+ + VK +S S TWTP L SK L+ A+ +
Sbjct: 321 SSKHSSPVKGNSSISSTWTPSNLKIYHSKNSMGLMDLDALKTVASNSKYRTKPKNCKTYG 380
Query: 659 GGMSSLTSPETPXEF 703
G T E P ++
Sbjct: 381 DGTELDTLDELPVDY 395
>SPAC9E9.17c |||dubiuos|Schizosaccharomyces pombe|chr 1|||Manual
Length = 72
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 345 RSYEQIFPRYDSFLNFAGYSFSDFLLVSVNIGTVDVSVANVN 220
RS E + D LN +F+DF ++N+G + +S N
Sbjct: 29 RSREFSVIQKDEELNVLEINFNDFYRTTLNVGELSMSTRKTN 70
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/40 (40%), Positives = 20/40 (50%)
Frame = +2
Query: 587 ESQLQLEAAGEVAAARDCQAGRQSGGMSSLTSPETPXEFC 706
ESQ L AA E A+ CQ G ++ M L + P FC
Sbjct: 773 ESQSNL-AAAERQASTYCQLGNETIFMLKLFTSSIPKAFC 811
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 25.8 bits (54), Expect = 8.6
Identities = 41/152 (26%), Positives = 66/152 (43%), Gaps = 20/152 (13%)
Frame = +1
Query: 118 IPVVTFNNGESIPVFGLGT------WKSKPGEVTQAV-KDAIDIGYRHIDCAYVYG---N 267
IP N +++P G G + E QAV A D+G D + +YG N
Sbjct: 3 IPTRKIGN-DTVPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGAN 61
Query: 268 EKEVGEAI--TSKIKE-GVVTR---EDLFITSKLWNTFHRPDLVXGXLQETLDNLNVQYL 429
E+ +G T + KE + T+ E T +L + + PD + L +L L + +
Sbjct: 62 EECIGRWFKQTGRRKEIFLATKFGYEKNPETGEL-SLNNEPDYIEKALDLSLKRLGIDCI 120
Query: 430 DLYLIH----WPQAYKEDGSLFPTXQSGKIQF 513
DLY +H K G+L ++GKI++
Sbjct: 121 DLYYVHRFSGETPIEKIMGALKKCVEAGKIRY 152
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,934,528
Number of Sequences: 5004
Number of extensions: 55024
Number of successful extensions: 194
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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