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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_I06
         (987 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    36   0.012
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ...    33   0.047
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te...    32   0.14 
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    29   1.3  
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb...    27   4.1  
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma...    27   4.1  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    27   4.1  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   5.4  
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc...    26   7.1  
SPBC19F8.04c |||nuclease|Schizosaccharomyces pombe|chr 2|||Manual      26   7.1  
SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa...    26   7.1  

>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 35.5 bits (78), Expect = 0.012
 Identities = 22/59 (37%), Positives = 22/59 (37%)
 Frame = -2

Query: 983 GRXGGXXGGXXXXXGXXKKXRGXGGXRXXRGPXGGXXGXXXGADGXXXXGGXPGGXGGG 807
           G  GG  G      G      G GG     G  GG  G   G  G    GG PGG GGG
Sbjct: 208 GGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGG--GLGGFGGGPGGFGGG 264



 Score = 32.7 bits (71), Expect = 0.082
 Identities = 21/59 (35%), Positives = 22/59 (37%)
 Frame = -2

Query: 983 GRXGGXXGGXXXXXGXXKKXRGXGGXRXXRGPXGGXXGXXXGADGXXXXGGXPGGXGGG 807
           G  GG  GG     G      G GG     G  GG  G   G +G       PGG GGG
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGG------PGGFGGG 243



 Score = 31.5 bits (68), Expect = 0.19
 Identities = 22/58 (37%), Positives = 22/58 (37%)
 Frame = -2

Query: 983 GRXGGXXGGXXXXXGXXKKXRGXGGXRXXRGPXGGXXGXXXGADGXXXXGGXPGGXGG 810
           G  GG  GG     G      G GG     G  GG  G   G  G    GG PGG GG
Sbjct: 221 GGHGGFGGGPGGFEG------GPGGFGGGPGGFGGGLGGFGGGPGGF--GGGPGGHGG 270


>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 255

 Score = 33.5 bits (73), Expect = 0.047
 Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
 Frame = +1

Query: 121 MLAASAGVV--ELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQGKGSII 276
           ++ A+AG+    LS +  N+D+  K+    L G Y +A      ++ QGKGS+I
Sbjct: 91  VMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144


>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
           termination factor Reb1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 504

 Score = 31.9 bits (69), Expect = 0.14
 Identities = 13/59 (22%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
 Frame = +1

Query: 268 SIIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVRKYFPLNFR--THHGRKLCQDHLQK 438
           +II   V+N I+D+  +  ++C ++W G   + +R ++   ++  +H   K    H+++
Sbjct: 247 AIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRR 305


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 28.7 bits (61), Expect = 1.3
 Identities = 16/52 (30%), Positives = 18/52 (34%)
 Frame = +2

Query: 821 PXANPXXXXPHRPLXXPXXXPPXAPSXSXTPPXXAXFFXPXPXXXXXPPXPP 976
           P + P    P  P+  P   PP  PS    PP  A      P     P  PP
Sbjct: 428 PPSLPPSAPPSLPMGAPAA-PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478



 Score = 28.3 bits (60), Expect = 1.8
 Identities = 18/56 (32%), Positives = 20/56 (35%), Gaps = 1/56 (1%)
 Frame = +2

Query: 821 PXANPXXXXPHRPLXXPXXXPPXAPSXSXTPPXXAXFFXPXP-XXXXXPPXPPXXP 985
           P + P    P  P   P   P  AP+    PP  A    P P      PP PP  P
Sbjct: 420 PPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPP-SAPIAPPLPAGMPAAPPLPPAAP 474


>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 300

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = -1

Query: 246 FQALTDSTVVVAGEDAVVQFLLEVLV 169
           F  +T   V+V  EDAVV+F+L +LV
Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206


>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 918

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
 Frame = -1

Query: 384 WEVLSNNFLS--VADPQL---VAVLHGVPSLVNDQVVNYILD 274
           W+VL +++L+  ++ P     V  LHGV + VN  V +YI D
Sbjct: 188 WDVLFHDYLNETLSQPAFSFNVPDLHGVDNKVNQYVFDYIKD 229


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +1

Query: 169 NQDLEEKLYNSILTGDYDSAVRQSLE 246
           + DLE+ +  ++LTGD  SAV+  LE
Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 26.6 bits (56), Expect = 5.4
 Identities = 17/58 (29%), Positives = 17/58 (29%)
 Frame = +2

Query: 812 LXXPXANPXXXXPHRPLXXPXXXPPXAPSXSXTPPXXAXFFXPXPXXXXXPPXPPXXP 985
           L  P   P       P   P   PP AP     PP       P       PP PP  P
Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP---PPGVAGAGPPPPPPPPP 783


>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
           Thi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +2

Query: 134 ARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRAW 244
           AR SLNY ++   + SRRN    ++P      S + W
Sbjct: 622 ARESLNYLKSFNKQLSRRNAPDINNPIADFQNSFQNW 658


>SPBC19F8.04c |||nuclease|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 230

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 2/76 (2%)
 Frame = +1

Query: 268 SIIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVRKYFPLNFRTHH--GRKLCQDHLQKL 441
           +I +N +NN  ++ R+    + Y   VG+G          NFR +H  G +L   H  + 
Sbjct: 38  TIPKNYLNNSTVENRKYKTMFGYVTRVGDGD---------NFRFYHTPGGRLLGWHWLRK 88

Query: 442 QPRSEARFHNQSLEMR 489
            P S +   N+++ +R
Sbjct: 89  VPCSRSDLSNETISVR 104


>SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 889

 Score = 26.2 bits (55), Expect = 7.1
 Identities = 11/34 (32%), Positives = 18/34 (52%)
 Frame = +1

Query: 271 IIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVR 372
           +++++  NL I    N +EY   LW  NG  I +
Sbjct: 447 VLKDIFFNLQIGVTFNILEYLRHLWSNNGDAIAK 480


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,965,217
Number of Sequences: 5004
Number of extensions: 54224
Number of successful extensions: 186
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 509282430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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