BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_G20 (933 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 0.38 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 0.59 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.94 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 24 1.6 SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 28 2.2 SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyc... 27 3.8 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 6.6 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 6.6 SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6 |Schizosaccharo... 26 8.7 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect(2) = 0.38 Identities = 11/27 (40%), Positives = 11/27 (40%) Frame = +3 Query: 825 PPPPXPXXXETXGXXPXPXXXXSXPTP 905 PPPP P G P P S P P Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPP 363 Score = 25.8 bits (54), Expect = 8.7 Identities = 12/36 (33%), Positives = 12/36 (33%) Frame = +3 Query: 735 PPQXXXPXXXLPLXWNXXGPPXXPXXQPXPPPPPXP 842 PP PL P P P PPP P P Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482 Score = 21.0 bits (42), Expect(2) = 0.38 Identities = 6/8 (75%), Positives = 7/8 (87%) Frame = +3 Query: 813 QPXPPPPP 836 +P PPPPP Sbjct: 310 RPPPPPPP 317 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 24.2 bits (50), Expect(2) = 0.59 Identities = 14/34 (41%), Positives = 15/34 (44%) Frame = +3 Query: 735 PPQXXXPXXXLPLXWNXXGPPXXPXXQPXPPPPP 836 PP P LP +N PP P PPPPP Sbjct: 173 PPSAAAPATSLPSDYN---PP------PPPPPPP 197 Score = 23.8 bits (49), Expect(2) = 0.59 Identities = 11/37 (29%), Positives = 12/37 (32%) Frame = +3 Query: 816 PXPPPPPXPXXXETXGXXPXPXXXXSXPTPXFSXXPP 926 P PPPPP P + P S PP Sbjct: 189 PPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPP 225 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 29.1 bits (62), Expect = 0.94 Identities = 15/49 (30%), Positives = 15/49 (30%) Frame = +3 Query: 732 PPPQXXXPXXXLPLXWNXXGPPXXPXXQPXPPPPPXPXXXETXGXXPXP 878 PPP P PP P PPPPP P P P Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780 Score = 25.8 bits (54), Expect = 8.7 Identities = 9/17 (52%), Positives = 9/17 (52%) Frame = +3 Query: 792 PPXXPXXQPXPPPPPXP 842 PP P PPPPP P Sbjct: 767 PPGVAGAGPPPPPPPPP 783 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 24.2 bits (50), Expect(2) = 1.6 Identities = 9/21 (42%), Positives = 9/21 (42%) Frame = +3 Query: 816 PXPPPPPXPXXXETXGXXPXP 878 P PPPPP P G P Sbjct: 945 PPPPPPPPPLVSAAGGKFVSP 965 Score = 22.2 bits (45), Expect(2) = 1.6 Identities = 7/11 (63%), Positives = 7/11 (63%) Frame = +3 Query: 804 PXXQPXPPPPP 836 P P PPPPP Sbjct: 942 PAFPPPPPPPP 952 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 27.9 bits (59), Expect = 2.2 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = -3 Query: 643 SGXSRQPSIIXTXVHFIFNFHVDKIWRISF 554 S PS + T + +F+ H D++W+IS+ Sbjct: 192 SDYKADPSELPTKEYHVFHDHSDEVWQISY 221 >SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 150 Score = 27.1 bits (57), Expect = 3.8 Identities = 14/49 (28%), Positives = 16/49 (32%) Frame = +3 Query: 732 PPPQXXXPXXXLPLXWNXXGPPXXPXXQPXPPPPPXPXXXETXGXXPXP 878 PPP P + PP +P P PP ET P P Sbjct: 79 PPPYTAEPEARYNSTIHNPMPPMSQAYRPPPTEPPTVPAYETSSEFPPP 127 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 26.2 bits (55), Expect = 6.6 Identities = 11/23 (47%), Positives = 12/23 (52%), Gaps = 2/23 (8%) Frame = +3 Query: 780 NXXGPPXXPXXQPX--PPPPPXP 842 N PP P +P PPPPP P Sbjct: 8 NPPPPPPPPGFEPPSQPPPPPPP 30 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 26.2 bits (55), Expect = 6.6 Identities = 13/35 (37%), Positives = 14/35 (40%) Frame = -3 Query: 835 GGGGGXGXXXGXXGGPXXFQXRGXXXXGFXXWGGG 731 GG GG G GG F G G +GGG Sbjct: 195 GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGG 229 >SPAC1F3.01 |rrp6|SPAC3H8.11|exosome subunit Rrp6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 777 Score = 25.8 bits (54), Expect = 8.7 Identities = 16/54 (29%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = -3 Query: 328 DLVHH-HEIFVGFHVCVAVLAGLDVEVLGDFVVLSFIVDLVNVVEKRQNLLLLF 170 DL HH + F GF VC+ ++ + + + D + L ++ +NVV N++ +F Sbjct: 243 DLEHHDYRSFRGF-VCLMQISNREKDWIVDTLELREELEALNVVFTNPNIIKVF 295 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,888,654 Number of Sequences: 5004 Number of extensions: 55306 Number of successful extensions: 241 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 211 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 473333082 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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