BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_G17 (962 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 207 2e-54 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 207 2e-54 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 147 2e-36 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 88 2e-18 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 75 2e-14 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 73 5e-14 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 69 1e-12 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 39 0.001 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.034 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.034 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.034 SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 31 0.32 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.56 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 1.7 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 3.9 SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil... 27 5.2 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 9.1 SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 26 9.1 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 207 bits (505), Expect = 2e-54 Identities = 107/185 (57%), Positives = 130/185 (70%), Gaps = 1/185 (0%) Frame = +2 Query: 188 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 367 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 368 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 544 DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEV Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115 Query: 545 TAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXY 724 TAALRVTDGAL TETVLRQA+ ERI+P++ +NK+DR Y Sbjct: 116 TAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELY 175 Query: 725 XTFQR 739 F R Sbjct: 176 QNFAR 180 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 207 bits (505), Expect = 2e-54 Identities = 107/185 (57%), Positives = 130/185 (70%), Gaps = 1/185 (0%) Frame = +2 Query: 188 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 367 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 368 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 544 DEQ+R +TIKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEV Sbjct: 61 ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115 Query: 545 TAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXY 724 TAALRVTDGAL TETVLRQA+ ERI+P++ +NK+DR Y Sbjct: 116 TAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELY 175 Query: 725 XTFQR 739 F R Sbjct: 176 QNFAR 180 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 147 bits (356), Expect = 2e-36 Identities = 80/160 (50%), Positives = 108/160 (67%) Frame = +2 Query: 206 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 385 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 386 CITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 565 IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ Sbjct: 67 GITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLC 118 Query: 566 DGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685 DGA T TVLRQA +RIK IL +NKMDR Sbjct: 119 DGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR 158 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 88.2 bits (209), Expect = 2e-18 Identities = 61/171 (35%), Positives = 86/171 (50%), Gaps = 7/171 (4%) Frame = +2 Query: 194 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF-- 355 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I G+ Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100 Query: 356 -TDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF 532 D + E+++ ITI+S A +E + N Q+ EK + IN+ID+PGH+DF Sbjct: 101 KMDFMELEREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDF 158 Query: 533 SSEVTAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685 + EV ALRV DGA+ T TV RQ + I F+NKMDR Sbjct: 159 TIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 74.9 bits (176), Expect = 2e-14 Identities = 54/151 (35%), Positives = 77/151 (50%), Gaps = 2/151 (1%) Frame = +2 Query: 239 NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAI 412 +IRN+ +IAH+D GK+TLT+ ++ G + +T T D E+ R ITI S AI Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86 Query: 413 SMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 592 S + +QR INLID+PGH DF+ EV ++ V DGA+ Sbjct: 87 SFTWR------------NQR--------INLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126 Query: 593 XXXXXXXTETVLRQAIAERIKPILFMNKMDR 685 T+ V +QA I ++F+NKMDR Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 73.3 bits (172), Expect = 5e-14 Identities = 48/157 (30%), Positives = 78/157 (49%) Frame = +2 Query: 212 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 391 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106 Query: 392 TIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 571 T+K+ SM + + +L+NLID+PGHVDF +EV +L +G Sbjct: 107 TVKAQTCSMIYYYH----------------GQSYLLNLIDTPGHVDFRAEVMHSLAACEG 150 Query: 572 ALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMD 682 + T + A ++ + I +NK+D Sbjct: 151 CILLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVD 187 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 68.5 bits (160), Expect = 1e-12 Identities = 46/157 (29%), Positives = 75/157 (47%), Gaps = 1/157 (0%) Frame = +2 Query: 218 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 394 G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++ Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191 Query: 395 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 574 IKST +++ D + K+ F ID+PGHVDF EV A + ++DG Sbjct: 192 IKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDGV 236 Query: 575 LXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685 + T +++ AI + +L +NK+DR Sbjct: 237 VLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDR 273 Score = 26.2 bits (55), Expect = 6.9 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = +1 Query: 40 LKILIVVXDSFYFDVDLRHPALARVLFQSVRHCWRMVVG 156 ++ LI V DS F+ DLR + + Q V W++V G Sbjct: 884 VRALIPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVPG 922 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 38.7 bits (86), Expect = 0.001 Identities = 38/152 (25%), Positives = 60/152 (39%), Gaps = 1/152 (0%) Frame = +2 Query: 230 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 409 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108 Query: 410 ISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXX 589 + +E + +D PGH D+ + DGA+ Sbjct: 109 VE--YETANRHYAH------------------VDCPGHADYIKNMITGAATMDGAIIVVS 148 Query: 590 XXXXXXXXTETVLRQAIAERIKPI-LFMNKMD 682 T L A +K I +++NK+D Sbjct: 149 ATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.034 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.034 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.034 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 30.7 bits (66), Expect = 0.32 Identities = 28/117 (23%), Positives = 52/117 (44%), Gaps = 1/117 (0%) Frame = +2 Query: 203 VDEIRGMMDKKR-NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQ 379 +DE M D+ R I + K+ +T L S+ ++A A R+ D + + Sbjct: 435 IDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTILNSRTSVLAAANPIFGRYDDMKTPGE 494 Query: 380 DRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTA 550 + I +ST +S F D++FI + E ++ ++I+ ++ SSE A Sbjct: 495 N--IDFQSTILSRF------DMIFIVKDEHDETKDRNIARHVINLHTNLQESSETLA 543 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 29.9 bits (64), Expect = 0.56 Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%) Frame = +2 Query: 251 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 418 ++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231 Query: 419 FFELEEK 439 FE K Sbjct: 232 AFEAMRK 238 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 28.3 bits (60), Expect = 1.7 Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 1/111 (0%) Frame = +2 Query: 248 NMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFE 427 N+ I HVD GKSTL +++ G++ R E + E + S A+ E Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV-DKRTMEK--IEREAKEAGKESWYLSWALDSTSE 296 Query: 428 LEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF-SSEVTAALRVTDGAL 577 EK E + F +L+D+PGH + ++ + A + G L Sbjct: 297 EREKGKTVEVGRAYFETEHRRF--SLLDAPGHKGYVTNMINGASQADIGVL 345 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 27.1 bits (57), Expect = 3.9 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = -3 Query: 537 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 361 EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++ Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125 >SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 195 Score = 26.6 bits (56), Expect = 5.2 Identities = 14/44 (31%), Positives = 22/44 (50%) Frame = -2 Query: 244 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKG 113 DIPL + P D + RE + RW + +L + Y ++ F G Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFG 145 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.8 bits (54), Expect = 9.1 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -3 Query: 489 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 382 P S +S W+ V+NTK SFS ++M +L+ Q+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203 >SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 25.8 bits (54), Expect = 9.1 Identities = 30/129 (23%), Positives = 51/129 (39%), Gaps = 1/129 (0%) Frame = -3 Query: 399 LMVMQRSCSSLRVSVKRVSPALAPAMIPALETNESVRVDLP*STWAITDMLRIFRFLSII 220 LMV+QR+ +L + P ++ AL+ ++ L + L++ RF S + Sbjct: 839 LMVLQRNAKNLSTCAP-LQPKTRVVILQALKKVDAKAQSLYLEIRKMESQLQVLRFESDL 897 Query: 219 PRISSTVKFTILDGL*FLFY*TNYHTPAM-AYALKKDPCEGRMSQVDVKIKRVXDNNQNL 43 + S +D + P++ + + E S DV DNN NL Sbjct: 898 DKCISLESSNDIDDRPSSTSPSTLKNPSINSINRQTHDVEKESSDRDVPHLLDPDNNFNL 957 Query: 42 KGIPRSSES 16 KG P + S Sbjct: 958 KGNPENPSS 966 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,086,501 Number of Sequences: 5004 Number of extensions: 60018 Number of successful extensions: 179 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 493304942 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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