SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_G17
         (962 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   207   2e-54
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   207   2e-54
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...   147   2e-36
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    88   2e-18
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce...    75   2e-14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po...    73   5e-14
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    69   1e-12
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    39   0.001
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...    34   0.034
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...    34   0.034
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...    34   0.034
SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi...    31   0.32 
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch...    30   0.56 
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    28   1.7  
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    27   3.9  
SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil...    27   5.2  
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl...    26   9.1  
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang...    26   9.1  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  207 bits (505), Expect = 2e-54
 Identities = 107/185 (57%), Positives = 130/185 (70%), Gaps = 1/185 (0%)
 Frame = +2

Query: 188 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 367
           MV FT +E+R +M K  N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1   MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60

Query: 368 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 544
            DEQ+R +TIKSTAIS+F E+ + D+      D +E ++   FL+NLIDSPGHVDFSSEV
Sbjct: 61  ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115

Query: 545 TAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXY 724
           TAALRVTDGAL            TETVLRQA+ ERI+P++ +NK+DR            Y
Sbjct: 116 TAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELY 175

Query: 725 XTFQR 739
             F R
Sbjct: 176 QNFAR 180


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  207 bits (505), Expect = 2e-54
 Identities = 107/185 (57%), Positives = 130/185 (70%), Gaps = 1/185 (0%)
 Frame = +2

Query: 188 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 367
           MV FT +E+R +M K  N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR
Sbjct: 1   MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60

Query: 368 KDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEV 544
            DEQ+R +TIKSTAIS+F E+ + D+      D +E ++   FL+NLIDSPGHVDFSSEV
Sbjct: 61  ADEQERGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEV 115

Query: 545 TAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXY 724
           TAALRVTDGAL            TETVLRQA+ ERI+P++ +NK+DR            Y
Sbjct: 116 TAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELY 175

Query: 725 XTFQR 739
             F R
Sbjct: 176 QNFAR 180


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score =  147 bits (356), Expect = 2e-36
 Identities = 80/160 (50%), Positives = 108/160 (67%)
 Frame = +2

Query: 206 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 385
           +++  +   + NIRN +++AHVDHGK+TL DSL++  GII+   AG  RF D R+DE  R
Sbjct: 7   EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66

Query: 386 CITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 565
            IT+KS+AIS+FF++       I+  D++ + EK +LINLIDSPGHVDFSSEV++A R+ 
Sbjct: 67  GITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLC 118

Query: 566 DGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685
           DGA             T TVLRQA  +RIK IL +NKMDR
Sbjct: 119 DGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR 158


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 88.2 bits (209), Expect = 2e-18
 Identities = 61/171 (35%), Positives = 86/171 (50%), Gaps = 7/171 (4%)
 Frame = +2

Query: 194 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF-- 355
           N  + E     DKKR   IRN+ + AH+D GK+T T+ ++   G I       G+     
Sbjct: 41  NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGA 100

Query: 356 -TDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF 532
             D  + E+++ ITI+S A    +E     +    N  Q+   EK + IN+ID+PGH+DF
Sbjct: 101 KMDFMELEREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDF 158

Query: 533 SSEVTAALRVTDGALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685
           + EV  ALRV DGA+            T TV RQ     +  I F+NKMDR
Sbjct: 159 TIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209


>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 813

 Score = 74.9 bits (176), Expect = 2e-14
 Identities = 54/151 (35%), Positives = 77/151 (50%), Gaps = 2/151 (1%)
 Frame = +2

Query: 239 NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAI 412
           +IRN+ +IAH+D GK+TLT+ ++   G  +     +T  T  D    E+ R ITI S AI
Sbjct: 27  SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86

Query: 413 SMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 592
           S  +             +QR        INLID+PGH DF+ EV  ++ V DGA+     
Sbjct: 87  SFTWR------------NQR--------INLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126

Query: 593 XXXXXXXTETVLRQAIAERIKPILFMNKMDR 685
                  T+ V +QA    I  ++F+NKMDR
Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157


>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 646

 Score = 73.3 bits (172), Expect = 5e-14
 Identities = 48/157 (30%), Positives = 78/157 (49%)
 Frame = +2

Query: 212 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 391
           +RG+   +  +RN +VIAH+DHGKSTL+D ++   G+I        +F D  + E+ R I
Sbjct: 50  VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106

Query: 392 TIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 571
           T+K+   SM +                    + +L+NLID+PGHVDF +EV  +L   +G
Sbjct: 107 TVKAQTCSMIYYYH----------------GQSYLLNLIDTPGHVDFRAEVMHSLAACEG 150

Query: 572 ALXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMD 682
            +            T +    A ++ +  I  +NK+D
Sbjct: 151 CILLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVD 187


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 68.5 bits (160), Expect = 1e-12
 Identities = 46/157 (29%), Positives = 75/157 (47%), Gaps = 1/157 (0%)
 Frame = +2

Query: 218 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 394
           G++    ++R+  V  H+ HGKS L D LV          +    R+TDT   E++R ++
Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191

Query: 395 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 574
           IKST +++               D + K+   F    ID+PGHVDF  EV A + ++DG 
Sbjct: 192 IKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDGV 236

Query: 575 LXXXXXXXXXXXXTETVLRQAIAERIKPILFMNKMDR 685
           +            T  +++ AI   +  +L +NK+DR
Sbjct: 237 VLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDR 273



 Score = 26.2 bits (55), Expect = 6.9
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 40   LKILIVVXDSFYFDVDLRHPALARVLFQSVRHCWRMVVG 156
            ++ LI V DS  F+ DLR     + + Q V   W++V G
Sbjct: 884  VRALIPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVPG 922


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 38/152 (25%), Positives = 60/152 (39%), Gaps = 1/152 (0%)
 Frame = +2

Query: 230 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 409
           KK ++ N+  I HVDHGK+TLT ++      +  A   +    D   +E+ R ITI S  
Sbjct: 50  KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108

Query: 410 ISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXX 589
           +   +E   +                      +D PGH D+   +       DGA+    
Sbjct: 109 VE--YETANRHYAH------------------VDCPGHADYIKNMITGAATMDGAIIVVS 148

Query: 590 XXXXXXXXTETVLRQAIAERIKPI-LFMNKMD 682
                   T   L  A    +K I +++NK+D
Sbjct: 149 ATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180


>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.034
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.034
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.034
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 224 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 319
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit
           Mcm5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 30.7 bits (66), Expect = 0.32
 Identities = 28/117 (23%), Positives = 52/117 (44%), Gaps = 1/117 (0%)
 Frame = +2

Query: 203 VDEIRGMMDKKR-NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQ 379
           +DE   M D+ R  I        +   K+ +T  L S+  ++A A     R+ D +   +
Sbjct: 435 IDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTILNSRTSVLAAANPIFGRYDDMKTPGE 494

Query: 380 DRCITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTA 550
           +  I  +ST +S F      D++FI   +  E  ++    ++I+   ++  SSE  A
Sbjct: 495 N--IDFQSTILSRF------DMIFIVKDEHDETKDRNIARHVINLHTNLQESSETLA 543


>SPBC1271.15c |||translation initiation factor
           IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 686

 Score = 29.9 bits (64), Expect = 0.56
 Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
 Frame = +2

Query: 251 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 418
           ++++ HVDHGK+TL D+   K+ I +    G T+    FT    D+  + IT   T   M
Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231

Query: 419 FFELEEK 439
            FE   K
Sbjct: 232 AFEAMRK 238


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 28.3 bits (60), Expect = 1.7
 Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 1/111 (0%)
 Frame = +2

Query: 248 NMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFE 427
           N+  I HVD GKSTL  +++   G++   R  E    +    E  +     S A+    E
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV-DKRTMEK--IEREAKEAGKESWYLSWALDSTSE 296

Query: 428 LEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF-SSEVTAALRVTDGAL 577
             EK           E   + F  +L+D+PGH  + ++ +  A +   G L
Sbjct: 297 EREKGKTVEVGRAYFETEHRRF--SLLDAPGHKGYVTNMINGASQADIGVL 345


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 27.1 bits (57), Expect = 3.9
 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
 Frame = -3

Query: 537 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 361
           EE  + P +  K  KK     S    F+  T   +S S++NI+ A+DL+ +  S S  ++
Sbjct: 66  EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125


>SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 195

 Score = 26.6 bits (56), Expect = 5.2
 Identities = 14/44 (31%), Positives = 22/44 (50%)
 Frame = -2

Query: 244 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKG 113
           DIPL +  P D + RE  + RW  + +L   + Y ++   F  G
Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFG 145


>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
           endonuclease Cce1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 258

 Score = 25.8 bits (54), Expect = 9.1
 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
 Frame = -3

Query: 489 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 382
           P S +S W+  V+NTK SFS     ++M  +L+  Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203


>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
            factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1082

 Score = 25.8 bits (54), Expect = 9.1
 Identities = 30/129 (23%), Positives = 51/129 (39%), Gaps = 1/129 (0%)
 Frame = -3

Query: 399  LMVMQRSCSSLRVSVKRVSPALAPAMIPALETNESVRVDLP*STWAITDMLRIFRFLSII 220
            LMV+QR+  +L      + P     ++ AL+  ++    L      +   L++ RF S +
Sbjct: 839  LMVLQRNAKNLSTCAP-LQPKTRVVILQALKKVDAKAQSLYLEIRKMESQLQVLRFESDL 897

Query: 219  PRISSTVKFTILDGL*FLFY*TNYHTPAM-AYALKKDPCEGRMSQVDVKIKRVXDNNQNL 43
             +  S      +D        +    P++ +   +    E   S  DV      DNN NL
Sbjct: 898  DKCISLESSNDIDDRPSSTSPSTLKNPSINSINRQTHDVEKESSDRDVPHLLDPDNNFNL 957

Query: 42   KGIPRSSES 16
            KG P +  S
Sbjct: 958  KGNPENPSS 966


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,086,501
Number of Sequences: 5004
Number of extensions: 60018
Number of successful extensions: 179
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 493304942
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -