BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_G08
(963 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 37 0.004
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 36 0.006
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 33 0.045
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.079
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.24
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.0
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 5.2
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 37.1 bits (82), Expect = 0.004
Identities = 17/48 (35%), Positives = 18/48 (37%)
Frame = +1
Query: 817 PPXPPXVHXPPPXXPPXPXXPXXPLTLXSXPSPPXXXXXXFXPXPXXP 960
PP PP V P P P P P P+ P PP P P P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 28.3 bits (60), Expect = 1.7
Identities = 20/67 (29%), Positives = 24/67 (35%), Gaps = 7/67 (10%)
Frame = +1
Query: 715 PFPPFXPXPXTLXXXXSPXHPPXGLXXSRXXLPXPPXPPXV-------HXPPPXXPPXPX 873
P P P P + P PP G+ + P PP PP V + P P P P
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP-PPPPPPPAVSAGGSRYYAPAPQAEPEPK 804
Query: 874 XPXXPLT 894
LT
Sbjct: 805 IDETSLT 811
Score = 27.9 bits (59), Expect = 2.3
Identities = 13/37 (35%), Positives = 17/37 (45%), Gaps = 3/37 (8%)
Frame = +1
Query: 763 SPXHPPXGLXX---SRXXLPXPPXPPXVHXPPPXXPP 864
SP PP + + +P PP P + PPP PP
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
Score = 26.2 bits (55), Expect = 6.9
Identities = 16/50 (32%), Positives = 17/50 (34%)
Frame = +1
Query: 715 PFPPFXPXPXTLXXXXSPXHPPXGLXXSRXXLPXPPXPPXVHXPPPXXPP 864
P PP P T P PP + P PP PPP PP
Sbjct: 734 PPPPAVIVP-TPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 36.3 bits (80), Expect = 0.006
Identities = 20/56 (35%), Positives = 22/56 (39%), Gaps = 4/56 (7%)
Frame = +1
Query: 763 SPXHPPXGLXXSRXXLPXPPXPPXVHXPPPXXPP----XPXXPXXPLTLXSXPSPP 918
+P P + P PP PP PPP PP P P PL S PS P
Sbjct: 1689 TPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 31.1 bits (67), Expect = 0.24
Identities = 22/74 (29%), Positives = 26/74 (35%), Gaps = 2/74 (2%)
Frame = +3
Query: 744 NAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXXXXPXHXPLLXLPP-- 917
NA AP + +PP P P P P + P + P P P LPP
Sbjct: 1468 NAPAPSSAPAPPAPVSQLPPAVPNVPVPSMI----PSVAQQPPSSVAPATAPSSTLPPSQ 1523
Query: 918 XXXXXXLSPXPPXP 959
SP PP P
Sbjct: 1524 SSFAHVPSPAPPAP 1537
Score = 28.3 bits (60), Expect = 1.7
Identities = 14/43 (32%), Positives = 18/43 (41%), Gaps = 3/43 (6%)
Frame = +3
Query: 732 PPSXNAXAPX---LSXSPPXRSXPFPXXXPXXPXPXLXSXPPP 851
PP +A P +S PP + P P P P P L + P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 33.5 bits (73), Expect = 0.045
Identities = 15/52 (28%), Positives = 21/52 (40%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXP 869
P+P+ PP ++ P + P +S P P P PPP S P
Sbjct: 159 PIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAP 210
Score = 30.7 bits (66), Expect = 0.32
Identities = 20/64 (31%), Positives = 22/64 (34%), Gaps = 2/64 (3%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSX-SPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXXXXP- 887
P+P+ PP AP S S P P P P P L P S P P
Sbjct: 166 PIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAPPA 225
Query: 888 XHXP 899
H P
Sbjct: 226 GHAP 229
Score = 30.3 bits (65), Expect = 0.42
Identities = 19/60 (31%), Positives = 23/60 (38%), Gaps = 2/60 (3%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXP--PPXXSPXPXXXXXP 887
P P++ PPS + P S +PP S P P P S P P P P P
Sbjct: 145 PRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPP 204
Score = 27.5 bits (58), Expect = 3.0
Identities = 22/81 (27%), Positives = 26/81 (32%), Gaps = 2/81 (2%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXP--XPXLXSXPPPXXSPXPXXXXXP 887
P L PP+ P + P + P P P P P P SP P P
Sbjct: 133 PQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP-PSAPSLP 191
Query: 888 XHXPLLXLPPXXXXXXLSPXP 950
P +PP LS P
Sbjct: 192 SAVP--PMPPKVPPPPLSQAP 210
Score = 26.6 bits (56), Expect = 5.2
Identities = 17/62 (27%), Positives = 22/62 (35%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXXXXPXH 893
P A PP+ + + +PP S P P P P + P P P P P
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPS-KAPPIPSSLPPPAQPAAPVK 182
Query: 894 XP 899
P
Sbjct: 183 SP 184
Score = 26.6 bits (56), Expect = 5.2
Identities = 13/35 (37%), Positives = 14/35 (40%)
Frame = +1
Query: 811 PXPPXPPXVHXPPPXXPPXPXXPXXPLTLXSXPSP 915
P PP P PP PP P P P + P P
Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIP-PPSPASAPPIP 161
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.7 bits (71), Expect = 0.079
Identities = 24/88 (27%), Positives = 30/88 (34%), Gaps = 5/88 (5%)
Frame = +3
Query: 711 IPVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXS-----PXPXX 875
+ VP + PS P S + P P P P P P + + P P S P P
Sbjct: 1109 VAVPPVPAPSGAPPVPKPSVAAPP--VPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSV 1166
Query: 876 XXXPXHXPLLXLPPXXXXXXLSPXPPXP 959
P P +PP P P P
Sbjct: 1167 AAPPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Score = 31.1 bits (67), Expect = 0.24
Identities = 23/79 (29%), Positives = 28/79 (35%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXXXXPXH 893
PVP + +A P + S S P P P P P S PP P P P
Sbjct: 1051 PVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAP---SGIPPV--PKPSVAAPPVP 1105
Query: 894 XPLLXLPPXXXXXXLSPXP 950
P + +PP P P
Sbjct: 1106 KPSVAVPPVPAPSGAPPVP 1124
Score = 29.9 bits (64), Expect = 0.56
Identities = 23/87 (26%), Positives = 27/87 (31%), Gaps = 4/87 (4%)
Frame = +3
Query: 711 IPVPALXPP----SXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXX 878
IPVP+ PP + P S P P P P S PP +P
Sbjct: 1034 IPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAP---SG 1090
Query: 879 XXPXHXPLLXLPPXXXXXXLSPXPPXP 959
P P + PP P P P
Sbjct: 1091 IPPVPKPSVAAPPVPKPSVAVPPVPAP 1117
Score = 29.5 bits (63), Expect = 0.74
Identities = 26/84 (30%), Positives = 30/84 (35%), Gaps = 5/84 (5%)
Frame = +3
Query: 714 PVPALXPPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXS----PXP-XXX 878
PVPA P S P S +PP P P P P P + + P P S P P
Sbjct: 1065 PVPA--PSSEIPSIPAPSGAPPV---PAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSG 1119
Query: 879 XXPXHXPLLXLPPXXXXXXLSPXP 950
P P + PP P P
Sbjct: 1120 APPVPKPSVAAPPVPVPSGAPPVP 1143
Score = 27.1 bits (57), Expect = 3.9
Identities = 21/84 (25%), Positives = 25/84 (29%), Gaps = 1/84 (1%)
Frame = +3
Query: 711 IPVPALX-PPSXNAXAPXLSXSPPXRSXPFPXXXPXXPXPXLXSXPPPXXSPXPXXXXXP 887
+P P+ PP A PP + P P P S PP P P P
Sbjct: 1171 VPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV--PTPSAGLPP 1228
Query: 888 XHXPLLXLPPXXXXXXLSPXPPXP 959
P PP +P P
Sbjct: 1229 VPVPTAKAPPVPAPSSEAPSVSTP 1252
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.1 bits (67), Expect = 0.24
Identities = 19/66 (28%), Positives = 21/66 (31%), Gaps = 1/66 (1%)
Frame = +1
Query: 724 PFXPXPXTLXXXXSPXHPPXGLXXSRXXLPX-PPXPPXVHXPPPXXPPXPXXPXXPLTLX 900
P P P +L P PP P PP PP PP P P P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 901 SXPSPP 918
+ P P
Sbjct: 475 APPPAP 480
Score = 30.7 bits (66), Expect = 0.32
Identities = 18/60 (30%), Positives = 24/60 (40%)
Frame = +1
Query: 739 PXTLXXXXSPXHPPXGLXXSRXXLPXPPXPPXVHXPPPXXPPXPXXPXXPLTLXSXPSPP 918
P + +P PP G SR P P PP + PP P P L + + +PP
Sbjct: 392 PPAIPGRSAPALPPLG-NASRTSTPPVPTPPSL--PPSAPPSLPPSAPPSLPMGAPAAPP 448
Score = 29.5 bits (63), Expect = 0.74
Identities = 17/56 (30%), Positives = 20/56 (35%), Gaps = 3/56 (5%)
Frame = +1
Query: 721 PPFXPXPXTLXXXXSPXHPPXG--LXXSRXXLPX-PPXPPXVHXPPPXXPPXPXXP 879
PP P + +P PP +P PP PP PPP P P P
Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 26.6 bits (56), Expect = 5.2
Identities = 20/82 (24%), Positives = 22/82 (26%), Gaps = 6/82 (7%)
Frame = +1
Query: 733 PXPXTLXXXXSPXHPPXGLXXSRXXLPXPPXPP------XVHXPPPXXPPXPXXPXXPLT 894
P P + PP G S LP PP PP + PP P P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Query: 895 LXSXPSPPXXXXXXFXPXPXXP 960
PP P P
Sbjct: 371 STGRQPPPLSSSRAVSNPPAPP 392
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.5 bits (58), Expect = 3.0
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 811 PXPPXPPXVHXPPPXXPPXP 870
P PP PP PP PP P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPP 28
Score = 27.1 bits (57), Expect = 3.9
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = +1
Query: 817 PPXPPXVHXPPPXXPPXPXXP 879
PP PP PP PP P P
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 5.2
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +1
Query: 763 SPXHPPXGLXXSRXXLPXPPXPPXVHXPP 849
SP PP LP PP PP PP
Sbjct: 220 SPEIPPTYTPKQADPLPAPPPPPPPTLPP 248
Score = 22.6 bits (46), Expect(2) = 7.9
Identities = 18/75 (24%), Positives = 23/75 (30%), Gaps = 6/75 (8%)
Frame = +1
Query: 709 EYPFPPFXPXPXTLXXXXSPXHPPXGLXXSRXXLPXPPXPPXVHX------PPPXXPPXP 870
+Y PP P P + + + P S + P PP P P PP P
Sbjct: 186 DYNPPPPPPPPPAVEDQAADANEPDDYYSSGRAVS-PEIPPTYTPKQADPLPAPPPPPPP 244
Query: 871 XXPXXPLTLXSXPSP 915
P P P
Sbjct: 245 TLPPQSTNTSQLPMP 259
Score = 21.4 bits (43), Expect(2) = 7.9
Identities = 8/21 (38%), Positives = 10/21 (47%)
Frame = +1
Query: 532 AFXPLGLRPPLTSIPKXXTPP 594
+F P P TS+P PP
Sbjct: 170 SFQPPSAAAPATSLPSDYNPP 190
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,618,215
Number of Sequences: 5004
Number of extensions: 46516
Number of successful extensions: 266
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 493304942
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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