BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_F01 (911 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.92 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.9 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 27 4.9 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 22 9.1 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 29.1 bits (62), Expect = 0.92 Identities = 24/104 (23%), Positives = 27/104 (25%), Gaps = 4/104 (3%) Frame = +3 Query: 585 PPKIXAXYXXFPXNTTRXSTXSXXPPTPPXXPSXXXPSXP--XXXXXXSXXPXXXXXXXX 758 PP+ P + S P PP P P+ P S P Sbjct: 365 PPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLP 424 Query: 759 XXXXPXPPXXXPXXXPXXXXXXP--XPXXPXPPPXPPXXXXXPP 884 P P P P P P P PP P PP Sbjct: 425 PSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468 Score = 28.3 bits (60), Expect = 1.6 Identities = 20/78 (25%), Positives = 21/78 (26%), Gaps = 3/78 (3%) Frame = +2 Query: 659 PHSPPXTLXXXXXXXPXXXXXLLXPXPXXXXPPXXXPXXP---PXXXPXXXSXPXXXPXP 829 P PP P L P PP P P P P S P P P Sbjct: 401 PALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLP 460 Query: 830 XXXPXSPXXXXXXXXXPP 883 P +P PP Sbjct: 461 AGMPAAPPLPPAAPAPPP 478 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 26.6 bits (56), Expect = 4.9 Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 7/74 (9%) Frame = +1 Query: 172 RRDAPDFFKDIEHHTKEFHKTLXQQFNSL-----TKSKDAQD--FXQGLEGRLRVRAATA 330 ++ DFFK + ++ H TL ++ NSL TKS A + F GL L ++ Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNW-LSLKLNLL 114 Query: 331 QRLRQESPAXRSET 372 RL+ + R+ T Sbjct: 115 LRLQGRMNSARTNT 128 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 26.6 bits (56), Expect = 4.9 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 351 SSXALGDANGKAKEALEQSRQNIERTAEE 437 SS G NG+AKE E+ R ++T E+ Sbjct: 468 SSAKQGKGNGRAKETPEEKRARKKKTKED 496 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 22.2 bits (45), Expect(2) = 9.1 Identities = 7/11 (63%), Positives = 7/11 (63%) Frame = +3 Query: 831 PXXPXPPPXPP 863 P P PPP PP Sbjct: 6 PGNPPPPPPPP 16 Score = 21.4 bits (43), Expect(2) = 9.1 Identities = 7/13 (53%), Positives = 7/13 (53%) Frame = +3 Query: 846 PPPXPPXXXXXPP 884 PPP PP PP Sbjct: 9 PPPPPPPPGFEPP 21 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,113,960 Number of Sequences: 5004 Number of extensions: 31373 Number of successful extensions: 138 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 90 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 462505890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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