Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_E23
(967 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.009
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 31 0.18
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.24
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 29 0.62
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.74
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.98
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 29 1.3
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.3
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.7
SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 9.1
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.9 bits (79), Expect = 0.009
Identities = 19/47 (40%), Positives = 19/47 (40%)
Frame = +2
Query: 824 PTPPXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPPP 964
PTP P P P PPPP P P PP P PPPP
Sbjct: 742 PTPAPAPIPV---PPPAPIMGGPPPP--PPPPGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.7
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 863 PSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPPP 964
P P P P P P P P PPPP
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 27.9 bits (59), Expect = 2.3
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 863 PSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPPP 964
P P P P P P P P PPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP 766
Score = 26.6 bits (56), Expect = 5.2
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 893 PPPXXPXPXXPXXXXPPXPXPPP 961
PPP P P P P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPP 754
Score = 26.2 bits (55), Expect = 6.9
Identities = 15/47 (31%), Positives = 15/47 (31%)
Frame = +3
Query: 822 TQPPPXXPXPXPGXXXXTPXXXXXPPPXXXXXPXPXXXXPXPPXPPP 962
T P P P P P PP P P PP PPP
Sbjct: 743 TPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP------PPPPPPPP 783
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 31.5 bits (68), Expect = 0.18
Identities = 16/45 (35%), Positives = 16/45 (35%)
Frame = +2
Query: 830 PPXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPPP 964
PP P P P PP P P P P PP P P P
Sbjct: 107 PPLPNEPVPEEPLP----GEPPLPDEPVPEEPLPGEPPLPNEPVP 147
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.24
Identities = 16/50 (32%), Positives = 16/50 (32%), Gaps = 2/50 (4%)
Frame = +2
Query: 821 HPTPPXXXXPAXRXPSPHXXXXXPPPPXX--PXPXXPXXXXPPXPXPPPP 964
HP P P PPPP P P P P PPPP
Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 28.3 bits (60), Expect = 1.7
Identities = 15/44 (34%), Positives = 15/44 (34%)
Frame = +2
Query: 824 PTPPXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXP 955
PTPP P P P PP P P P P P P
Sbjct: 1705 PTPPPP--PMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 29.5 bits (63), Expect = 0.74
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +2
Query: 890 PPPPXXPXPXXPXXXXPPXPXPPPP 964
PP P P PP P PPPP
Sbjct: 173 PPSAAAPATSLPSDYNPPPPPPPPP 197
Score = 25.4 bits (53), Expect(2) = 0.62
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = +2
Query: 830 PPXXXXPAXRXPSPHXXXXXPPPP 901
PP PA PS + PPPP
Sbjct: 173 PPSAAAPATSLPSDYNPPPPPPPP 196
Score = 22.6 bits (46), Expect(2) = 0.62
Identities = 9/24 (37%), Positives = 9/24 (37%)
Frame = +2
Query: 893 PPPXXPXPXXPXXXXPPXPXPPPP 964
PP P P PP P P P
Sbjct: 224 PPTYTPKQADPLPAPPPPPPPTLP 247
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.5 bits (63), Expect = 0.74
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 893 PPPXXPXPXXPXXXXPPXPXPPPP 964
PP P P P PP PPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
Score = 28.7 bits (61), Expect = 1.3
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +2
Query: 890 PPPPXXPXPXXPXXXXPPXPXPPPP 964
PP P P P P P PPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +2
Query: 890 PPPPXXPXPXXPXXXXPPXPXPPPP 964
PPPP P P PP PPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPP---PPPP 30
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.98
Identities = 13/44 (29%), Positives = 13/44 (29%)
Frame = +3
Query: 828 PPPXXPXPXPGXXXXTPXXXXXPPPXXXXXPXPXXXXPXPPXPP 959
PP P P P PP P P PP PP
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPP 471
Score = 27.9 bits (59), Expect = 2.3
Identities = 15/46 (32%), Positives = 16/46 (34%)
Frame = +2
Query: 824 PTPPXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPP 961
P PP P+ R P P PP P P PP P P
Sbjct: 231 PIPPSI--PSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRP 274
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/43 (34%), Positives = 16/43 (37%), Gaps = 4/43 (9%)
Frame = +2
Query: 848 PAXRXPSPHXXXXXPPP----PXXPXPXXPXXXXPPXPXPPPP 964
PA P+P PPP P P P PP PP P
Sbjct: 37 PAFMEPAPVSKKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAP 79
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/44 (34%), Positives = 15/44 (34%)
Frame = +2
Query: 824 PTPPXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXP 955
P PP P PS PP P P P PP P P
Sbjct: 1190 PVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPT-PSAGLPPVPVP 1232
Score = 26.6 bits (56), Expect = 5.2
Identities = 13/45 (28%), Positives = 14/45 (31%)
Frame = +3
Query: 828 PPPXXPXPXPGXXXXTPXXXXXPPPXXXXXPXPXXXXPXPPXPPP 962
PP P P + P P P P PP PPP
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Score = 25.8 bits (54), Expect = 9.1
Identities = 14/45 (31%), Positives = 15/45 (33%), Gaps = 3/45 (6%)
Frame = +2
Query: 830 PPXXXXPAXRXPS---PHXXXXXPPPPXXPXPXXPXXXXPPXPXP 955
PP P+ PS P P P P P PP P P
Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKP 1107
Score = 25.8 bits (54), Expect = 9.1
Identities = 14/45 (31%), Positives = 14/45 (31%), Gaps = 2/45 (4%)
Frame = +3
Query: 834 PXXPXPXPGXXXXTPXXXXXPP--PXXXXXPXPXXXXPXPPXPPP 962
P P P G PP P P P PP PPP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 28.3 bits (60), Expect = 1.7
Identities = 19/49 (38%), Positives = 19/49 (38%), Gaps = 2/49 (4%)
Frame = -2
Query: 963 GGGGXGXGGXXXX-GXXGXGXXG-GGGXXXXXXGXGXRXAGXXXXGGVG 823
GG G G GG G G G G GGG G G G GG G
Sbjct: 224 GGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
Score = 27.9 bits (59), Expect = 2.3
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 1/49 (2%)
Frame = -1
Query: 967 GXGGGXGGXGXXXXGXGXXXXX-GGGXXXXXGVXXXXPGXGXGXXGGGW 824
G GGG GG G G GGG G G G G GW
Sbjct: 225 GFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPGW 273
>SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 589
Score = 25.8 bits (54), Expect = 9.1
Identities = 13/43 (30%), Positives = 14/43 (32%)
Frame = +2
Query: 833 PXXXXPAXRXPSPHXXXXXPPPPXXPXPXXPXXXXPPXPXPPP 961
P P+ PS P P P P P P PPP
Sbjct: 145 PAATAPSEFMPSIPGFGNLPNPAMPPIPFLPFNPAAQPPFPPP 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,967,458
Number of Sequences: 5004
Number of extensions: 54874
Number of successful extensions: 231
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 495302128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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