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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_E16
         (996 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    36   0.009
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    33   0.063
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    30   0.58 
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz...    29   1.3  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    27   3.1  
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p...    27   4.1  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   4.1  
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar...    26   7.2  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    26   9.5  

>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 35.9 bits (79), Expect = 0.009
 Identities = 26/94 (27%), Positives = 34/94 (36%), Gaps = 3/94 (3%)
 Frame = +3

Query: 723 PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902
           P+P ++  P       PS    SP     P     PP+   + P  QP +  KSPP    
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASA-PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189

Query: 903 XP---PPXXPTFXXQTHPRLXLVXTPSRXSXXXP 995
            P   PP  P        +  +  T SR S   P
Sbjct: 190 LPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAP 223



 Score = 33.1 bits (72), Expect = 0.063
 Identities = 21/67 (31%), Positives = 25/67 (37%)
 Frame = +3

Query: 747 PAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSXXPPPXXPT 926
           P+A     P    L PP    PR    PP      P   P    K+PP  S  PPP  P 
Sbjct: 125 PSAPAPPTPQSE-LRPPTSAPPRPSIPPP-----SPASAPPIPSKAPPIPSSLPPPAQPA 178

Query: 927 FXXQTHP 947
              ++ P
Sbjct: 179 APVKSPP 185


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 33.1 bits (72), Expect = 0.063
 Identities = 25/98 (25%), Positives = 32/98 (32%), Gaps = 1/98 (1%)
 Frame = +2

Query: 620 AXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXPRRXPXXXPL-XXXPLTP 796
           A   P   +     TPP P P +     PP L  S P   + P   P   PL    P+ P
Sbjct: 400 APALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAP--PSLPMGAPAAPPLPPSAPIAP 457

Query: 797 PXXXSPRXXXPPPXPRXETXXSTHLXPQIPPXSLSXAP 910
           P         P P        +    P  P  S++  P
Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASIAELP 495



 Score = 32.7 bits (71), Expect = 0.083
 Identities = 33/130 (25%), Positives = 40/130 (30%), Gaps = 5/130 (3%)
 Frame = +2

Query: 569 TNEKLAPQDQGPAYDDFAXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXP 748
           +N  L P    P   + A   P          PP P+        PP L  S  +  + P
Sbjct: 332 SNSSLPPPPPPPR-SNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAV--SNP 388

Query: 749 RRXPXXXPLXXXPLTPPXXXSPRXXXPP--PXPRXETXXSTHLXPQIPPX---SLSXAPP 913
              P   P    P  PP   + R   PP    P         L P  PP        APP
Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448

Query: 914 XPSNXXXANP 943
            P +   A P
Sbjct: 449 LPPSAPIAPP 458


>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 168

 Score = 29.9 bits (64), Expect = 0.58
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +3

Query: 300 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 437
           KDG+++   +LN FAK L  +    + +A +   +    IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156


>SPBC646.09c |int6|yin6|translation initiation factor
           eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 28.7 bits (61), Expect = 1.3
 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
 Frame = +3

Query: 279 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 434
           +D + A+ +   SVLQ+L+   + +QG LG   N     AL Q + QN++   E
Sbjct: 79  EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 27.5 bits (58), Expect = 3.1
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -1

Query: 411 ASTVPKPPWPCRSRLRALPG 352
           AST+ K PWP  + L  +PG
Sbjct: 38  ASTLEKEPWPASTALLVMPG 57


>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 473

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 17/56 (30%), Positives = 21/56 (37%), Gaps = 3/56 (5%)
 Frame = +2

Query: 809 SPRXXXPPPXPRXETXXSTHLXPQI---PPXSLSXAPPXPSNXXXANPSXSXSCXN 967
           SP+   PP              P+I   PP S+   PP P     AN + S S  N
Sbjct: 321 SPKAQQPPAHLVQSAPVQRKTTPKIQRLPPSSIQIPPPKPMQKFPANAASSESPPN 376


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 27.1 bits (57), Expect = 4.1
 Identities = 13/46 (28%), Positives = 15/46 (32%)
 Frame = +2

Query: 704 PPXLXISFPIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841
           PP   +  P     P   P   P+   P  PP         PPP P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779


>SPAC2F7.03c |pom1||DYRK family protein kinase
           Pom1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1087

 Score = 26.2 bits (55), Expect = 7.2
 Identities = 12/33 (36%), Positives = 13/33 (39%)
 Frame = +2

Query: 827 PPPXPRXETXXSTHLXPQIPPXSLSXAPPXPSN 925
           PPP PR     S        P  +   PP PSN
Sbjct: 514 PPPVPRLSITRSQVSREPEKPEEIPSVPPLPSN 546


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 25.8 bits (54), Expect = 9.5
 Identities = 20/75 (26%), Positives = 25/75 (33%)
 Frame = +3

Query: 723  PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902
            P+P +   P A +   P      P P   P     PP    V P   P+S    PP +S 
Sbjct: 1470 PAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPP--SSVAPATAPSS--TLPPSQSS 1525

Query: 903  XPPPXXPTFXXQTHP 947
                  P      HP
Sbjct: 1526 FAHVPSPAPPAPQHP 1540



 Score = 25.8 bits (54), Expect = 9.5
 Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%)
 Frame = +2

Query: 686  AFDIDXPPXLXISF-PIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841
            A  +  PP    S  P   + P   P    +   P  PP    P    PPP P
Sbjct: 1684 AHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,747,225
Number of Sequences: 5004
Number of extensions: 44572
Number of successful extensions: 203
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 515273988
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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