BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_E16 (996 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 36 0.009 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.063 SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.58 SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.3 SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 3.1 SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 27 4.1 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.1 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 26 7.2 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 9.5 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 35.9 bits (79), Expect = 0.009 Identities = 26/94 (27%), Positives = 34/94 (36%), Gaps = 3/94 (3%) Frame = +3 Query: 723 PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902 P+P ++ P PS SP P PP+ + P QP + KSPP Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASA-PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189 Query: 903 XP---PPXXPTFXXQTHPRLXLVXTPSRXSXXXP 995 P PP P + + T SR S P Sbjct: 190 LPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAP 223 Score = 33.1 bits (72), Expect = 0.063 Identities = 21/67 (31%), Positives = 25/67 (37%) Frame = +3 Query: 747 PAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSXXPPPXXPT 926 P+A P L PP PR PP P P K+PP S PPP P Sbjct: 125 PSAPAPPTPQSE-LRPPTSAPPRPSIPPP-----SPASAPPIPSKAPPIPSSLPPPAQPA 178 Query: 927 FXXQTHP 947 ++ P Sbjct: 179 APVKSPP 185 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 33.1 bits (72), Expect = 0.063 Identities = 25/98 (25%), Positives = 32/98 (32%), Gaps = 1/98 (1%) Frame = +2 Query: 620 AXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXPRRXPXXXPL-XXXPLTP 796 A P + TPP P P + PP L S P + P P PL P+ P Sbjct: 400 APALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAP--PSLPMGAPAAPPLPPSAPIAP 457 Query: 797 PXXXSPRXXXPPPXPRXETXXSTHLXPQIPPXSLSXAP 910 P P P + P P S++ P Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASIAELP 495 Score = 32.7 bits (71), Expect = 0.083 Identities = 33/130 (25%), Positives = 40/130 (30%), Gaps = 5/130 (3%) Frame = +2 Query: 569 TNEKLAPQDQGPAYDDFAXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXP 748 +N L P P + A P PP P+ PP L S + + P Sbjct: 332 SNSSLPPPPPPPR-SNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAV--SNP 388 Query: 749 RRXPXXXPLXXXPLTPPXXXSPRXXXPP--PXPRXETXXSTHLXPQIPPX---SLSXAPP 913 P P P PP + R PP P L P PP APP Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448 Query: 914 XPSNXXXANP 943 P + A P Sbjct: 449 LPPSAPIAPP 458 >SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 29.9 bits (64), Expect = 0.58 Identities = 13/46 (28%), Positives = 25/46 (54%) Frame = +3 Query: 300 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 437 KDG+++ +LN FAK L + + +A + + IE+++ E Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156 >SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 28.7 bits (61), Expect = 1.3 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%) Frame = +3 Query: 279 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 434 +D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132 >SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 631 Score = 27.5 bits (58), Expect = 3.1 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 411 ASTVPKPPWPCRSRLRALPG 352 AST+ K PWP + L +PG Sbjct: 38 ASTLEKEPWPASTALLVMPG 57 >SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces pombe|chr 1|||Manual Length = 473 Score = 27.1 bits (57), Expect = 4.1 Identities = 17/56 (30%), Positives = 21/56 (37%), Gaps = 3/56 (5%) Frame = +2 Query: 809 SPRXXXPPPXPRXETXXSTHLXPQI---PPXSLSXAPPXPSNXXXANPSXSXSCXN 967 SP+ PP P+I PP S+ PP P AN + S S N Sbjct: 321 SPKAQQPPAHLVQSAPVQRKTTPKIQRLPPSSIQIPPPKPMQKFPANAASSESPPN 376 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.1 bits (57), Expect = 4.1 Identities = 13/46 (28%), Positives = 15/46 (32%) Frame = +2 Query: 704 PPXLXISFPIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841 PP + P P P P+ P PP PPP P Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 26.2 bits (55), Expect = 7.2 Identities = 12/33 (36%), Positives = 13/33 (39%) Frame = +2 Query: 827 PPPXPRXETXXSTHLXPQIPPXSLSXAPPXPSN 925 PPP PR S P + PP PSN Sbjct: 514 PPPVPRLSITRSQVSREPEKPEEIPSVPPLPSN 546 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.8 bits (54), Expect = 9.5 Identities = 20/75 (26%), Positives = 25/75 (33%) Frame = +3 Query: 723 PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902 P+P + P A + P P P P PP V P P+S PP +S Sbjct: 1470 PAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPP--SSVAPATAPSS--TLPPSQSS 1525 Query: 903 XPPPXXPTFXXQTHP 947 P HP Sbjct: 1526 FAHVPSPAPPAPQHP 1540 Score = 25.8 bits (54), Expect = 9.5 Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%) Frame = +2 Query: 686 AFDIDXPPXLXISF-PIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841 A + PP S P + P P + P PP P PPP P Sbjct: 1684 AHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,747,225 Number of Sequences: 5004 Number of extensions: 44572 Number of successful extensions: 203 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 515273988 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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