BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_E16
(996 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 36 0.009
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.063
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.58
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.3
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 3.1
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 27 4.1
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.1
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 26 7.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 9.5
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 35.9 bits (79), Expect = 0.009
Identities = 26/94 (27%), Positives = 34/94 (36%), Gaps = 3/94 (3%)
Frame = +3
Query: 723 PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902
P+P ++ P PS SP P PP+ + P QP + KSPP
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASA-PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189
Query: 903 XP---PPXXPTFXXQTHPRLXLVXTPSRXSXXXP 995
P PP P + + T SR S P
Sbjct: 190 LPSAVPPMPPKVPPPPLSQAPVANTSSRPSSFAP 223
Score = 33.1 bits (72), Expect = 0.063
Identities = 21/67 (31%), Positives = 25/67 (37%)
Frame = +3
Query: 747 PAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSXXPPPXXPT 926
P+A P L PP PR PP P P K+PP S PPP P
Sbjct: 125 PSAPAPPTPQSE-LRPPTSAPPRPSIPPP-----SPASAPPIPSKAPPIPSSLPPPAQPA 178
Query: 927 FXXQTHP 947
++ P
Sbjct: 179 APVKSPP 185
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.063
Identities = 25/98 (25%), Positives = 32/98 (32%), Gaps = 1/98 (1%)
Frame = +2
Query: 620 AXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXPRRXPXXXPL-XXXPLTP 796
A P + TPP P P + PP L S P + P P PL P+ P
Sbjct: 400 APALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAP--PSLPMGAPAAPPLPPSAPIAP 457
Query: 797 PXXXSPRXXXPPPXPRXETXXSTHLXPQIPPXSLSXAP 910
P P P + P P S++ P
Sbjct: 458 PLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASIAELP 495
Score = 32.7 bits (71), Expect = 0.083
Identities = 33/130 (25%), Positives = 40/130 (30%), Gaps = 5/130 (3%)
Frame = +2
Query: 569 TNEKLAPQDQGPAYDDFAXXTPQXXDQGXXXTPPTPKPXAFDIDXPPXLXISFPIHTTXP 748
+N L P P + A P PP P+ PP L S + + P
Sbjct: 332 SNSSLPPPPPPPR-SNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAV--SNP 388
Query: 749 RRXPXXXPLXXXPLTPPXXXSPRXXXPP--PXPRXETXXSTHLXPQIPPX---SLSXAPP 913
P P P PP + R PP P L P PP APP
Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448
Query: 914 XPSNXXXANP 943
P + A P
Sbjct: 449 LPPSAPIAPP 458
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.58
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +3
Query: 300 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 437
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.3
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +3
Query: 279 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 434
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 3.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 411 ASTVPKPPWPCRSRLRALPG 352
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 473
Score = 27.1 bits (57), Expect = 4.1
Identities = 17/56 (30%), Positives = 21/56 (37%), Gaps = 3/56 (5%)
Frame = +2
Query: 809 SPRXXXPPPXPRXETXXSTHLXPQI---PPXSLSXAPPXPSNXXXANPSXSXSCXN 967
SP+ PP P+I PP S+ PP P AN + S S N
Sbjct: 321 SPKAQQPPAHLVQSAPVQRKTTPKIQRLPPSSIQIPPPKPMQKFPANAASSESPPN 376
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.1 bits (57), Expect = 4.1
Identities = 13/46 (28%), Positives = 15/46 (32%)
Frame = +2
Query: 704 PPXLXISFPIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841
PP + P P P P+ P PP PPP P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 26.2 bits (55), Expect = 7.2
Identities = 12/33 (36%), Positives = 13/33 (39%)
Frame = +2
Query: 827 PPPXPRXETXXSTHLXPQIPPXSLSXAPPXPSN 925
PPP PR S P + PP PSN
Sbjct: 514 PPPVPRLSITRSQVSREPEKPEEIPSVPPLPSN 546
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 9.5
Identities = 20/75 (26%), Positives = 25/75 (33%)
Frame = +3
Query: 723 PSPFTQRXPAAXLXXXPSXXXLSPPPXRXPRAXXXPPLXRGVKPGXQPTSXHKSPPXRSX 902
P+P + P A + P P P P PP V P P+S PP +S
Sbjct: 1470 PAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPP--SSVAPATAPSS--TLPPSQSS 1525
Query: 903 XPPPXXPTFXXQTHP 947
P HP
Sbjct: 1526 FAHVPSPAPPAPQHP 1540
Score = 25.8 bits (54), Expect = 9.5
Identities = 15/53 (28%), Positives = 18/53 (33%), Gaps = 1/53 (1%)
Frame = +2
Query: 686 AFDIDXPPXLXISF-PIHTTXPRRXPXXXPLXXXPLTPPXXXSPRXXXPPPXP 841
A + PP S P + P P + P PP P PPP P
Sbjct: 1684 AHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,747,225
Number of Sequences: 5004
Number of extensions: 44572
Number of successful extensions: 203
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 515273988
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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