BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_D10 (1019 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.065 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.8 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 5.6 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 7.4 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 33.1 bits (72), Expect = 0.065 Identities = 14/36 (38%), Positives = 14/36 (38%) Frame = +1 Query: 553 PXXGXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTPP 660 P G PVPP S P P P P P PP Sbjct: 1203 PSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238 Score = 29.9 bits (64), Expect = 0.60 Identities = 13/37 (35%), Positives = 14/37 (37%) Frame = -3 Query: 267 GAPPXPXXFFXXXPPPPPRGGXPXXPXXPXPIXPXXP 157 GAPP P P P P G P P + P P Sbjct: 1157 GAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193 Score = 29.1 bits (62), Expect = 1.1 Identities = 13/35 (37%), Positives = 13/35 (37%) Frame = +1 Query: 553 PXXGXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTP 657 P G PVPP S P P P P P P Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218 Score = 28.7 bits (61), Expect = 1.4 Identities = 12/31 (38%), Positives = 13/31 (41%) Frame = +1 Query: 571 PVPPKSXXXPXXXPKGPTPXAPXPXXPTPPG 663 PVP S P P P P + P P P G Sbjct: 1051 PVPKSSSGAPSAPPPVPAPSSEIPSIPAPSG 1081 Score = 28.7 bits (61), Expect = 1.4 Identities = 12/36 (33%), Positives = 13/36 (36%) Frame = +1 Query: 553 PXXGXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTPP 660 P G PVP + P P P P P PP Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP 1209 Score = 27.5 bits (58), Expect = 3.2 Identities = 12/33 (36%), Positives = 12/33 (36%) Frame = +1 Query: 562 GXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTPP 660 G PVP S P P P P P PP Sbjct: 1081 GAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPP 1113 Score = 26.2 bits (55), Expect = 7.4 Identities = 12/36 (33%), Positives = 12/36 (33%) Frame = +1 Query: 553 PXXGXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTPP 660 P PVP S P P P P P PP Sbjct: 1145 PSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180 Score = 26.2 bits (55), Expect = 7.4 Identities = 12/36 (33%), Positives = 12/36 (33%) Frame = +1 Query: 553 PXXGXXPVPPKSXXXPXXXPKGPTPXAPXPXXPTPP 660 P PVP S P P P P P PP Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPP 1228 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.3 bits (60), Expect = 1.8 Identities = 11/35 (31%), Positives = 14/35 (40%) Frame = +1 Query: 571 PVPPKSXXXPXXXPKGPTPXAPXPXXPTPPGRGXK 675 P PP + P P P P P P+ P G + Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDR 1749 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 26.6 bits (56), Expect = 5.6 Identities = 12/32 (37%), Positives = 12/32 (37%) Frame = -3 Query: 261 PPXPXXFFXXXPPPPPRGGXPXXPXXPXPIXP 166 PP PPPPP G P P P P Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 26.2 bits (55), Expect = 7.4 Identities = 10/24 (41%), Positives = 11/24 (45%) Frame = -3 Query: 228 PPPPPRGGXPXXPXXPXPIXPXXP 157 PPPPP P P P+ P P Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAP 756 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 26.2 bits (55), Expect = 7.4 Identities = 15/38 (39%), Positives = 15/38 (39%), Gaps = 1/38 (2%) Frame = +2 Query: 158 GXXGXXGXGXXGXXGXPPR-GGGGGXXXKXKXGXGGAP 268 G G G G G G P GGG G G GG P Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGP 258 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.148 0.511 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,679,191 Number of Sequences: 5004 Number of extensions: 18176 Number of successful extensions: 77 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 63 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 531251476 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.9 bits)
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