BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_D09 (882 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g16260.1 68414.m01947 protein kinase family protein contains ... 30 2.4 At3g58570.1 68416.m06528 DEAD box RNA helicase, putative similar... 29 5.4 At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase / 3-meth... 28 9.5 At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha ch... 28 9.5 >At1g16260.1 68414.m01947 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 720 Score = 29.9 bits (64), Expect = 2.4 Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Frame = +3 Query: 264 RSSGLWERATKDFLVKVVT-TGXFFNDDRGKLTGQAYGTRVLGPGGDSTSYGGRLD 428 R+ GL + FL V T F ++D T + +R+LG GG T Y G L+ Sbjct: 355 RNGGLLLQQQTSFLHGSVNRTKVFSSNDLENATDRFNASRILGQGGQGTVYKGMLE 410 >At3g58570.1 68416.m06528 DEAD box RNA helicase, putative similar to SP|O00571 DEAD-box protein 3 (Helicase-like protein 2) {Homo sapiens}, DEAD box RNA helicase DDX3 [Homo sapiens] GI:3523150; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 646 Score = 28.7 bits (61), Expect = 5.4 Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 2/31 (6%) Frame = +3 Query: 336 NDDRGKLTG--QAYGTRVLGPGGDSTSYGGR 422 N+DRG G YG R G GG + YGGR Sbjct: 52 NNDRGGYGGANSGYGGRGQGYGGRGSGYGGR 82 >At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase / 3-methyl-2-oxobutanoate dehydrogenase / branched-chain alpha-keto acid dehydrogenase E1 beta subunit (DIN4) identical to branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] GI:7021286 Length = 358 Score = 27.9 bits (59), Expect = 9.5 Identities = 19/63 (30%), Positives = 31/63 (49%) Frame = -3 Query: 343 SSLKNXPVVTTFTKKSLVALSQSPEDLPSPHXPCPK*QSREYREGVGLTLKIGW*SDTSL 164 SS+++ V F K L Q+ ED+P P ++ REG +TL +GW + ++ Sbjct: 194 SSIRDPNPVVFFEPKWLYR--QAVEDVPEDDYMIPLSEAEVMREGSDITL-VGWGAQLTI 250 Query: 163 RNQ 155 Q Sbjct: 251 MEQ 253 >At1g03090.2 68414.m00284 methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) nearly identical to SP|Q42523 Methylcrotonyl-CoA carboxylase alpha chain, mitochondrial precursor (EC 6.4.1.4) (3-Methylcrotonyl-CoA carboxylase 1) (MCCase alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase alpha subunit) {Arabidopsis thaliana} Length = 734 Score = 27.9 bits (59), Expect = 9.5 Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 1/60 (1%) Frame = +3 Query: 180 HQPIFKVSPTPSRYSRL-CHLGQGKWGEGRSSGLWERATKDFLVKVVTTGXFFNDDRGKL 356 HQ I + +P P+ + +LGQ R+ G + T +F+V + +F + +L Sbjct: 271 HQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAGTVEFIVDTESDQFYFMEMNTRL 330 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,786,066 Number of Sequences: 28952 Number of extensions: 310341 Number of successful extensions: 771 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 752 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 770 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 2077687200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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