Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_D08
(1013 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 101 1e-22
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 94 3e-20
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 75 2e-14
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 61 3e-10
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 58 3e-09
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 43 6e-05
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 39 0.001
SPAC17G8.13c |mst2||histone acetyltransferase Mst2|Schizosacchar... 28 1.8
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 28 2.4
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 27 3.2
SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces po... 27 3.2
SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor |Schizo... 27 4.2
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 27 4.2
SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces po... 26 7.4
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 26 7.4
SPAC3C7.14c |obr1|apt1, uhp1|ubiquitinated histone-like protein ... 26 9.7
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 101 bits (243), Expect = 1e-22
Identities = 58/154 (37%), Positives = 88/154 (57%), Gaps = 10/154 (6%)
Frame = +3
Query: 141 LSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGR 320
L +G IP + LGTW GK ++T+ AV AL+AGY HIDTA+ YG E ++G
Sbjct: 17 LPNGDKIPSIGLGTWRS----GK-----DETKNAVCAALKAGYRHIDTAHIYGNEKEIGE 67
Query: 321 ALTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWPIAMF- 497
+ + G+ R I++T+KLW + H V AL K+L++LNLEY+D YLIHWP A+
Sbjct: 68 GIRES---GVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLS 124
Query: 498 ---------ENDTLLDNVDFLDTWRGMIDAKKLG 572
+ + + ++V +TW+ M + + G
Sbjct: 125 GPEELPRNEKGELIYEDVPIEETWQAMEELLETG 158
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 93.9 bits (223), Expect = 3e-20
Identities = 50/117 (42%), Positives = 70/117 (59%)
Frame = +3
Query: 141 LSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGR 320
L+ G IP + LGTW N T+ AV AL+ GY HID A YG ED+VG
Sbjct: 18 LADGSKIPGLGLGTWRSE---------PNQTKNAVKTALQYGYRHIDAAAIYGNEDEVGD 68
Query: 321 ALTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWPIA 491
+ + G+ R+ I++T+KLW + H E V AL K+L++L L+Y+D YLIHWP++
Sbjct: 69 GIKES---GVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVS 122
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 74.5 bits (175), Expect = 2e-14
Identities = 49/142 (34%), Positives = 74/142 (52%)
Frame = +3
Query: 159 IPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKF 338
+P +GT L + KG++ + D+ V AL AG+ HID A YG E++VG AL +
Sbjct: 12 VPAYGVGTALFKKEKGEINRTIVDS---VKNALAAGFIHIDCAEVYGNEEEVGVALKEA- 67
Query: 339 SEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWPIAMFENDTLLD 518
+ R +FIT+K+ H + + AL +SL L +Y+DLYL+H PI +E
Sbjct: 68 --NVPRSKLFITSKVM---HNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYEK----- 117
Query: 519 NVDFLDTWRGMIDAKKLGLTKS 584
+ + W+ M A GL S
Sbjct: 118 KIPISEGWKAMETALGTGLVHS 139
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 60.9 bits (141), Expect = 3e-10
Identities = 40/118 (33%), Positives = 63/118 (53%), Gaps = 1/118 (0%)
Frame = +3
Query: 135 LQLSSGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTAYKYGIEDQV 314
++L++G P A G+++ +R K +V AL+ GY HID+A Y E
Sbjct: 6 VKLNNGLKCPQFAYGSYMVNRTK---------CFDSVYAALQCGYRHIDSAQMYHNEADC 56
Query: 315 GRALTKKFSE-GLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWP 485
GRA+ K E G KRE I+ T+KL N + + ++ S++ L Y+DL+L+H P
Sbjct: 57 GRAILKFMEETGTKREDIWFTSKL-NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSP 113
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 57.6 bits (133), Expect = 3e-09
Identities = 36/88 (40%), Positives = 50/88 (56%), Gaps = 1/88 (1%)
Frame = +3
Query: 225 NDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKFSE-GLKRESIFITTKLWNSYHE 401
N+ V AL++GY HIDTA YG ED G+A+ + +KR IF+T+KL N +
Sbjct: 32 NECYGLVTQALDSGYRHIDTAAVYGNEDICGKAIVDWCEKNNVKRTDIFLTSKLANC-SD 90
Query: 402 REQVIPALRKSLENLNLEYVDLYLIHWP 485
A+R SL +L Y+DL+LI P
Sbjct: 91 YYSTRAAIRSSLHHLG-TYIDLFLIQSP 117
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 43.2 bits (97), Expect = 6e-05
Identities = 31/97 (31%), Positives = 47/97 (48%), Gaps = 5/97 (5%)
Frame = +3
Query: 228 DTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKFSEG--LKRESIFITTKLWNSYHE 401
D Q VI AL G ID+A Y E + +A+ + +KRE I + TK+ +S
Sbjct: 25 DCYQQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQG 84
Query: 402 REQVIPALRKSLENLNLEYVDLYLIH---WPIAMFEN 503
E+ A+ +SL +D+ LIH WP+ E+
Sbjct: 85 FERTWKAVEQSLRRTGRPKLDVVLIHSPKWPVRRIES 121
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 38.7 bits (86), Expect = 0.001
Identities = 38/122 (31%), Positives = 58/122 (47%), Gaps = 9/122 (7%)
Frame = +3
Query: 147 SGGNIPVVALGTWLGHRPKGKVVQVTNDTEQAVIYALEAGYTHIDTA--YKYGIEDQV-G 317
SG + +LG WL + +G V+ T+ + A + G DTA Y G + V G
Sbjct: 21 SGLKVSAFSLGGWLTYGNEGYDVE---HTKNCLKQAWDLGINTFDTAEIYSNGNSETVMG 77
Query: 318 RALTK---KFSEGLKRESIFIT--TKLWNSYH-EREQVIPALRKSLENLNLEYVDLYLIH 479
+A+ + SE + +F TKL N+ R+ +I L SL+ L L YVD+ + H
Sbjct: 78 KAIKELGWDRSEYVITTKVFFGAGTKLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAH 137
Query: 480 WP 485
P
Sbjct: 138 RP 139
>SPAC17G8.13c |mst2||histone acetyltransferase
Mst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 407
Score = 28.3 bits (60), Expect = 1.8
Identities = 23/73 (31%), Positives = 33/73 (45%), Gaps = 1/73 (1%)
Frame = +3
Query: 324 LTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIHWPIAM-FE 500
L KK+ LKR+ + K WN H ++V P L + L E + L+ I +
Sbjct: 335 LKKKYVLQLKRDELENVYKAWNIKHP-QRVNPKLLRWTPYLGEEQISNLLLKENILIPLP 393
Query: 501 NDTLLDNVDFLDT 539
LLDN LD+
Sbjct: 394 QKRLLDNSHHLDS 406
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 27.9 bits (59), Expect = 2.4
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +3
Query: 324 LTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEYVDLYLIH 479
L +K + LK+ IF T L ++ ++P + K + NLN + D Y IH
Sbjct: 77 LCRKLASTLKQRVIFFTRVLL-TFFCWTVLLPLIFKHVWNLNFKIGDTYTIH 127
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 27.5 bits (58), Expect = 3.2
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 580 LVRPSFLASIIPRHVSRKSTLSSKVSFSNI 491
L+ PSF A I RHV + T S+ V N+
Sbjct: 698 LIEPSFTAKIFARHVLLEKTKSTLVVKKNV 727
>SPBC1778.09 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 414
Score = 27.5 bits (58), Expect = 3.2
Identities = 19/73 (26%), Positives = 32/73 (43%)
Frame = +3
Query: 399 EREQVIPALRKSLENLNLEYVDLYLIHWPIAMFENDTLLDNVDFLDTWRGMIDAKKLGLT 578
+R + + L++ NLN+ I+ + + L+NVD +R + LG
Sbjct: 13 QRIRSLYRLQEDKNNLNIPEP----INGKVQEIDTKESLENVDRYGNYRSDFNVNNLGFY 68
Query: 579 KS*ASPTLTRTNP 617
K PTL + NP
Sbjct: 69 KVSKDPTLDKPNP 81
>SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 512
Score = 27.1 bits (57), Expect = 4.2
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = +3
Query: 225 NDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKFSEGL 350
N+ E +Y + A H+ AY+ I Q+G +T+ E +
Sbjct: 244 NEKEAYRLYKMAADLNHVQAAYRVAICLQMGFGVTQNTEEAI 285
>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
heterochromatin assembly Hrr1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1015
Score = 27.1 bits (57), Expect = 4.2
Identities = 12/50 (24%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +3
Query: 396 HEREQVIPALRKSLENLNLEYVDLYLIHWP--IAMFENDTLLDNVDFLDT 539
H+++++I + + N NLE+V L +H I + ++L +N +++ +
Sbjct: 482 HKKQRLIKQITNIMHNFNLEFVTLSYLHSKGIITTSQLESLRNNTEWISS 531
>SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 418
Score = 26.2 bits (55), Expect = 7.4
Identities = 17/34 (50%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = +3
Query: 225 NDTEQAVIYA---LEAGYTHIDTAYKYGIEDQVG 317
NDT A+ A LEAG THIDT+ GI ++ G
Sbjct: 227 NDTGMAIANAYCALEAGATHIDTSI-LGIGERNG 259
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 26.2 bits (55), Expect = 7.4
Identities = 36/144 (25%), Positives = 57/144 (39%), Gaps = 11/144 (7%)
Frame = +3
Query: 303 EDQVGRALTKKFSEGLKRESIFITTKLWNSYHEREQVIPALRKSLENLNLEY---VDLYL 473
+ Q+ A K E K KL S + + +L+KSLE +EY + +
Sbjct: 798 QQQLSDAARKVKFESEKESLSLSLQKLKESNEKMSNDLHSLQKSLEKSGIEYSSRIKTLM 857
Query: 474 IHWPIAMFENDTLLDNVDF----LDTWRGMIDAKKLGLTKS*ASPTLTRTNPEDHRQRTG 641
+ +N LLDN L G+I+ +K + A T + R+
Sbjct: 858 LEKQSLSEDNRKLLDNQQMMEIKLQELNGVIELEKQRFSTLEAKFTQQKNTSYSEREALL 917
Query: 642 EXSA--LQIELTS--TAHKFHLRN 701
E S LQ + TS + + + LRN
Sbjct: 918 ESSLSDLQSKHTSLESQYNYSLRN 941
>SPAC3C7.14c |obr1|apt1, uhp1|ubiquitinated histone-like protein
Uhp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 202
Score = 25.8 bits (54), Expect = 9.7
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +3
Query: 222 TNDTEQAVIYALEAGYTHIDTAYKYGIEDQVGRALTKKFSEGLKRE 359
T +T VIY+ + A K GIE G+A+ +F E L E
Sbjct: 3 TANTVAIVIYSTYGHVVKLAEAEKAGIEKAGGKAVIYQFPETLSPE 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,267,713
Number of Sequences: 5004
Number of extensions: 66706
Number of successful extensions: 198
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 527257104
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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