Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_D07
(999 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 129 7e-31
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 73 5e-14
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 66 1e-11
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 65 2e-11
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 58 2e-09
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 58 3e-09
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 56 6e-09
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 52 1e-07
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 48 3e-06
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 47 4e-06
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 46 8e-06
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 43 6e-05
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 32 0.15
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 30 0.59
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 129 bits (311), Expect = 7e-31
Identities = 56/137 (40%), Positives = 87/137 (63%), Gaps = 1/137 (0%)
Frame = +1
Query: 166 KRLMREAIELAEATEEYC-ARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMH 342
KRLM+E + L + E A+P +N+ EWH+ + GP T +EGG YHG ++ P +YP
Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67
Query: 343 PPHIILLTPNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTPAEGTIGSLD 522
PP I ++TP+GRF+ N ++CLS S HP++W PSW + T L+ L++FM T E T G +
Sbjct: 68 PPAIRMITPSGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFM-TSDEITTGGIV 126
Query: 523 YTPAERKILAKKSTNWS 573
+ + R+ AK + ++
Sbjct: 127 TSESTRRTYAKDTKRFN 143
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 73.3 bits (172), Expect = 5e-14
Identities = 42/113 (37%), Positives = 63/113 (55%), Gaps = 3/113 (2%)
Frame = +1
Query: 166 KRLMREAIELA-EATEEYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMH 342
KR+++E L + A P EDNL + T++GP+ + +EGG +H + LP EYPM
Sbjct: 5 KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMM 64
Query: 343 PPHIILLTPNGRFEINK--KICLSISGHHPETWQPSWSIRTALLALIAFMPTP 495
PP++ LT ++K +ICLS + W P+ IRT LL++ A M P
Sbjct: 65 PPNVRFLTKIYHPNVDKLGRICLSTL---KKDWSPALQIRTVLLSIQALMGAP 114
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 65.7 bits (153), Expect = 1e-11
Identities = 43/139 (30%), Positives = 68/139 (48%), Gaps = 14/139 (10%)
Frame = +1
Query: 148 TKCPGVKRLMREAIELAE-ATEEYCARPL-EDNLFEWHFTVQGPKGTEFEGGIYHGRILL 321
+K ++RLM+E EL E + A P ED+ F W +QGP GT FEGG+Y +
Sbjct: 2 SKAMALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKF 61
Query: 322 PKEYPMHPPHIILLTPNGRFEINK--KICLSI---SGHHP-------ETWQPSWSIRTAL 465
P +YP+ PP + + K +C+SI G P E W P S+ L
Sbjct: 62 PSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKIL 121
Query: 466 LALIAFMPTPAEGTIGSLD 522
L++++ + P + + ++D
Sbjct: 122 LSVMSMLAEPNDESGANID 140
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 64.9 bits (151), Expect = 2e-11
Identities = 35/114 (30%), Positives = 56/114 (49%), Gaps = 3/114 (2%)
Frame = +1
Query: 163 VKRLMREAIELAEATEEYC-ARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPM 339
+KR+ RE +L + C A P+ D+LF W T+ GP + + GG++ I P +YP
Sbjct: 3 LKRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPF 62
Query: 340 HPPHIILLTP--NGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 495
PP + T + N ICL I + W P+ +I LL++ + + P
Sbjct: 63 KPPKVNFTTRIYHPNINSNGSICLDIL---RDQWSPALTISKVLLSICSLLTDP 113
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 58.0 bits (134), Expect = 2e-09
Identities = 34/115 (29%), Positives = 63/115 (54%), Gaps = 7/115 (6%)
Frame = +1
Query: 163 VKRLMRE--AIELAEATEEYCARPLED-NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEY 333
+KRL +E + + + + +P+ D ++F W ++GP T +EGG + I + + Y
Sbjct: 5 IKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGY 64
Query: 334 PMHPPHIILLT----PNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFM 486
P+ PP + T PN + N ++C+ I H W P+WS+++A LA+I+ +
Sbjct: 65 PISPPSVYFQTKIVHPNISW-TNGEVCMDILKTH---WSPAWSLQSACLAIISLL 115
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 57.6 bits (133), Expect = 3e-09
Identities = 31/110 (28%), Positives = 57/110 (51%), Gaps = 13/110 (11%)
Frame = +1
Query: 232 EDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMHPPHIILLTP--NGRFEINKKICL 405
+ ++FEW + GP+ T +EGG +H + P++YP+ PP + T + N ++C+
Sbjct: 32 DKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCI 91
Query: 406 SISGHHP-----------ETWQPSWSIRTALLALIAFMPTPAEGTIGSLD 522
SI H P E W P S T L+++I+ + +P + + ++D
Sbjct: 92 SIL-HPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSPNDESPANID 140
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 56.4 bits (130), Expect = 6e-09
Identities = 34/123 (27%), Positives = 60/123 (48%), Gaps = 5/123 (4%)
Frame = +1
Query: 166 KRLMREAIELAE-ATEEYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMH 342
+RL +E +L E + ++DNLF W T GP + + GG +H + P +YP
Sbjct: 10 RRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQ 69
Query: 343 PPHIILLTP--NGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFM--PTPAEGTI 510
PP I T + F+ +CL+I + ++PS +R+ L ++ + P P + +
Sbjct: 70 PPTIEFTTRIYHPNFDSEGNVCLAIL--KQQVFKPSIKLRSVLEQILQLLREPNPDDPLV 127
Query: 511 GSL 519
S+
Sbjct: 128 ASI 130
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 52.4 bits (120), Expect = 1e-07
Identities = 36/114 (31%), Positives = 55/114 (48%), Gaps = 4/114 (3%)
Frame = +1
Query: 166 KRLMREAIELAEA-TEEYCARPLED-NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPM 339
KRL E + L + T A P D NL W T+ GP T +EG + + P YP
Sbjct: 31 KRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPY 90
Query: 340 HPPHIILLTP--NGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 495
PP II +P + +++ ICL I + W ++++T LL+L + + P
Sbjct: 91 SPPTIIFTSPMWHPNVDMSGNICLDIL---KDKWSAVYNVQTILLSLQSLLGEP 141
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 47.6 bits (108), Expect = 3e-06
Identities = 26/88 (29%), Positives = 42/88 (47%), Gaps = 2/88 (2%)
Frame = +1
Query: 238 NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMHPPHIILLTP--NGRFEINKKICLSI 411
+L W + G T +EGG+Y + P+EYP PP P + + +CLSI
Sbjct: 37 DLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSI 96
Query: 412 SGHHPETWQPSWSIRTALLALIAFMPTP 495
+ E W+P+ +I+ LL + + P
Sbjct: 97 L-NEEEGWKPAITIKQILLGIQDLLDDP 123
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 47.2 bits (107), Expect = 4e-06
Identities = 35/117 (29%), Positives = 59/117 (50%), Gaps = 8/117 (6%)
Frame = +1
Query: 169 RLMREAIELAEATEEYCAR----PLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYP 336
R R A ELA+ ++ A + D++ +GP+GT +EGG + I +P +YP
Sbjct: 5 RSRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYP 64
Query: 337 MHPPHIILLT----PNGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 495
PP + T PN + ICL I + W P +++++AL++L + + TP
Sbjct: 65 FRPPKMNFDTKIYHPNVSSQTG-AICLDIL---KDQWSPVYTMKSALISLQSLLCTP 117
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 46.0 bits (104), Expect = 8e-06
Identities = 27/113 (23%), Positives = 53/113 (46%), Gaps = 3/113 (2%)
Frame = +1
Query: 166 KRLMREAIELAEATEE-YCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMH 342
+RLMR+ + + A P+ DN+ W+ + GP T FE G + + ++YP
Sbjct: 7 RRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNK 66
Query: 343 PPHIILLTP--NGRFEINKKICLSISGHHPETWQPSWSIRTALLALIAFMPTP 495
PP + ++ + N ++CL I + W P++ + L ++ + + P
Sbjct: 67 PPLVKFVSTMFHPNVYANGELCLDILQNR---WSPTYDVAAILTSIQSLLNDP 116
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 43.2 bits (97), Expect = 6e-05
Identities = 25/85 (29%), Positives = 41/85 (48%), Gaps = 4/85 (4%)
Frame = +1
Query: 211 EYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMHPPHI----ILLTPNGR 378
+Y + DN+ E++ GP T + GGI+ + LP EYP P I + PN
Sbjct: 19 DYEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPN-I 77
Query: 379 FEINKKICLSISGHHPETWQPSWSI 453
E++ +CL + +TW P + +
Sbjct: 78 DELSGSVCLDVIN---QTWSPMFDM 99
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 31.9 bits (69), Expect = 0.15
Identities = 27/118 (22%), Positives = 51/118 (43%), Gaps = 3/118 (2%)
Frame = +1
Query: 157 PGVKRLMREAIELAEATEEYCARPLEDNLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYP 336
P R+ ++ +L + + P L H ++ +G ++GG + RI + YP
Sbjct: 23 PAQIRIQKDVTDLEIPSTMSTSWPDPIKLNVLHLEIRPDEGY-YKGGKFKFRIQIDDNYP 81
Query: 337 MHPPHIILLTP--NGRFEINKKICLSISGHHPETWQPSWSIRTALLAL-IAFMPTPAE 501
PP + L + +I +CL+I + W P ++ + L+ L F+ AE
Sbjct: 82 HDPPKVKCLNKIYHPNIDIEGNVCLNIL---RQDWNPVLNLNSILVGLQFLFLSPNAE 136
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 29.9 bits (64), Expect = 0.59
Identities = 17/59 (28%), Positives = 28/59 (47%), Gaps = 1/59 (1%)
Frame = +1
Query: 193 LAEATEEYCARPLED-NLFEWHFTVQGPKGTEFEGGIYHGRILLPKEYPMHPPHIILLT 366
L E++ Y +D L +W+ T+ GP + E IY +I YP PP + ++
Sbjct: 21 LGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVS 79
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,245,082
Number of Sequences: 5004
Number of extensions: 65480
Number of successful extensions: 162
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 517271174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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