Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_C21
(952 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 112 6e-26
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 112 6e-26
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 112 6e-26
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 63 6e-11
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 3e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 32 0.10
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 31 0.18
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.42
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 28 1.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 112 bits (270), Expect = 6e-26
Identities = 52/67 (77%), Positives = 54/67 (80%)
Frame = +1
Query: 196 IYKCGGIDXRTXEKFEKXAXXMGXGSFKYAWVLXXLKAXRERGIXIDIALWKFETSXYYV 375
IYKCGGID RT EKFEK A +G GSFKYAWVL LKA RERGI IDIALWKFET Y V
Sbjct: 28 IYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNV 87
Query: 376 TIIDAPG 396
T+IDAPG
Sbjct: 88 TVIDAPG 94
Score = 92.3 bits (219), Expect = 9e-20
Identities = 48/93 (51%), Positives = 58/93 (62%)
Frame = +3
Query: 399 RXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXLXVGVHXM 578
R F KNM GTSQADCA+LI+ GTGEFEAG SK+G TRE ALLA+ LGV L V V+ M
Sbjct: 96 RDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKM 155
Query: 579 DXXXXXYREPRFEXXXXXVSSYXTXXGXHPAXV 677
D + + RFE S++ G +P V
Sbjct: 156 D--TTGWSQARFEEIVKETSNFIKKVGFNPKTV 186
Score = 39.5 bits (88), Expect = 7e-04
Identities = 19/27 (70%), Positives = 20/27 (74%)
Frame = +3
Query: 114 MGXXXTXINIVVIGXVDSGKSTTTGSL 194
MG IN+VVIG VDSGKSTTTG L
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHL 27
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 112 bits (270), Expect = 6e-26
Identities = 52/67 (77%), Positives = 54/67 (80%)
Frame = +1
Query: 196 IYKCGGIDXRTXEKFEKXAXXMGXGSFKYAWVLXXLKAXRERGIXIDIALWKFETSXYYV 375
IYKCGGID RT EKFEK A +G GSFKYAWVL LKA RERGI IDIALWKFET Y V
Sbjct: 28 IYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNV 87
Query: 376 TIIDAPG 396
T+IDAPG
Sbjct: 88 TVIDAPG 94
Score = 92.7 bits (220), Expect = 7e-20
Identities = 49/93 (52%), Positives = 58/93 (62%)
Frame = +3
Query: 399 RXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXLXVGVHXM 578
R F KNM GTSQADCAVLI+ GTGEFEAG SK+G TRE ALLA+ LGV L V V+ M
Sbjct: 96 RDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKM 155
Query: 579 DXXXXXYREPRFEXXXXXVSSYXTXXGXHPAXV 677
D + + RFE S++ G +P V
Sbjct: 156 D--TTGWSQARFEEIVKETSNFIKKVGFNPKTV 186
Score = 39.5 bits (88), Expect = 7e-04
Identities = 19/27 (70%), Positives = 20/27 (74%)
Frame = +3
Query: 114 MGXXXTXINIVVIGXVDSGKSTTTGSL 194
MG IN+VVIG VDSGKSTTTG L
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHL 27
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 112 bits (270), Expect = 6e-26
Identities = 52/67 (77%), Positives = 54/67 (80%)
Frame = +1
Query: 196 IYKCGGIDXRTXEKFEKXAXXMGXGSFKYAWVLXXLKAXRERGIXIDIALWKFETSXYYV 375
IYKCGGID RT EKFEK A +G GSFKYAWVL LKA RERGI IDIALWKFET Y V
Sbjct: 28 IYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNV 87
Query: 376 TIIDAPG 396
T+IDAPG
Sbjct: 88 TVIDAPG 94
Score = 92.3 bits (219), Expect = 9e-20
Identities = 48/93 (51%), Positives = 58/93 (62%)
Frame = +3
Query: 399 RXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXLXVGVHXM 578
R F KNM GTSQADCA+LI+ GTGEFEAG SK+G TRE ALLA+ LGV L V V+ M
Sbjct: 96 RDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKM 155
Query: 579 DXXXXXYREPRFEXXXXXVSSYXTXXGXHPAXV 677
D + + RFE S++ G +P V
Sbjct: 156 D--TTGWSQARFEEIVKETSNFIKKVGFNPKTV 186
Score = 39.5 bits (88), Expect = 7e-04
Identities = 19/27 (70%), Positives = 20/27 (74%)
Frame = +3
Query: 114 MGXXXTXINIVVIGXVDSGKSTTTGSL 194
MG IN+VVIG VDSGKSTTTG L
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHL 27
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 62.9 bits (146), Expect = 6e-11
Identities = 31/82 (37%), Positives = 46/82 (56%)
Frame = +3
Query: 399 RXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXLXVGVHXM 578
+ + NM G SQAD VL+++A GEFEAG + G TRE A+LA G+ L V ++ M
Sbjct: 327 KGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKM 386
Query: 579 DXXXXXYREPRFEXXXXXVSSY 644
D + E R++ +S +
Sbjct: 387 DEPSVQWSEERYKECVDKLSMF 408
Score = 47.6 bits (108), Expect = 3e-06
Identities = 22/67 (32%), Positives = 35/67 (52%)
Frame = +1
Query: 196 IYKCGGIDXRTXEKFEKXAXXMGXGSFKYAWVLXXLKAXRERGIXIDIALWKFETSXYYV 375
++ G +D RT EK E+ A G S+ +W L RE+G +++ FET
Sbjct: 259 LFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRF 318
Query: 376 TIIDAPG 396
+++DAPG
Sbjct: 319 SLLDAPG 325
Score = 29.1 bits (62), Expect = 0.96
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +3
Query: 135 INIVVIGXVDSGKSTTTGSL 194
+NIV IG VD+GKST G++
Sbjct: 239 VNIVFIGHVDAGKSTLGGNI 258
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.0 bits (124), Expect = 3e-08
Identities = 31/82 (37%), Positives = 41/82 (50%)
Frame = +3
Query: 399 RXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXLXVGVHXM 578
R F M G S AD AVL+V + FE G +NG TRE A L LG+ + V V+ +
Sbjct: 266 RDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKL 325
Query: 579 DXXXXXYREPRFEXXXXXVSSY 644
D + E RF+ VS +
Sbjct: 326 D--LMSWSEDRFQEIKNIVSDF 345
Score = 53.6 bits (123), Expect = 4e-08
Identities = 25/67 (37%), Positives = 37/67 (55%)
Frame = +1
Query: 196 IYKCGGIDXRTXEKFEKXAXXMGXGSFKYAWVLXXLKAXRERGIXIDIALWKFETSXYYV 375
+++ G I+ R+ +K A G GSF YAW+L + R RG+ +D+A FE+
Sbjct: 198 MFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIY 257
Query: 376 TIIDAPG 396
I DAPG
Sbjct: 258 EIGDAPG 264
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +3
Query: 135 INIVVIGXVDSGKSTTTGSLDLQM 206
+++VV G VDSGKST G + ++
Sbjct: 178 VHLVVTGHVDSGKSTMLGRIMFEL 201
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 32.3 bits (70), Expect = 0.10
Identities = 21/68 (30%), Positives = 33/68 (48%)
Frame = +3
Query: 378 HH*CSWTRXFXKNMXXGTSQADCAVLIVAAGTGEFEAGXSKNGXTREXALLAFXLGVXXL 557
H C + KNM G + D A+++V+A G+ TRE LLA +GV +
Sbjct: 120 HVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMPQ-------TREHLLLARQVGVKQI 172
Query: 558 XVGVHXMD 581
V ++ +D
Sbjct: 173 VVYINKVD 180
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 31.5 bits (68), Expect = 0.18
Identities = 22/75 (29%), Positives = 28/75 (37%), Gaps = 4/75 (5%)
Frame = +2
Query: 647 HXXRVXPXXCPLSCPXPAGPATXCXXPPPQM-PXSKXXPXTLXT---AXSDRXAPXHXPX 814
H P P S P P PAT C PPQ+ P + P + + + + P
Sbjct: 201 HPIHNHPYSSPTSYPPPLCPATYCPSNPPQLAPATAIAPSSQSSQHKSVNYSVTPSSINN 260
Query: 815 HTXPPXPPXXTRPLP 859
HT P P LP
Sbjct: 261 HTAVPLSPTLAVWLP 275
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.42
Identities = 24/67 (35%), Positives = 26/67 (38%), Gaps = 2/67 (2%)
Frame = +2
Query: 665 PXXCPLSCPXPAGPAT-XCXXPPPQMPXSKXXPXTLXTAXSDRXAPXHXPXHTXPPXP-P 838
P PLS P PA PP SK P L +A AP T PP P P
Sbjct: 991 PPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSA----DAPPIPVPSTAPPVPIP 1046
Query: 839 XXTRPLP 859
T P+P
Sbjct: 1047 TSTPPVP 1053
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 28.3 bits (60), Expect = 1.7
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 289 VLXXLKAXRERGIXIDIALWKFETSXYYVTIIDAPG 396
V+ L A R+RGI I+ A F + +ID PG
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPG 102
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,838,801
Number of Sequences: 5004
Number of extensions: 21277
Number of successful extensions: 71
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 49
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 485316198
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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