Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_C13
(947 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 40 5e-04
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 39 0.001
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.31
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.7
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 2.9
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 8.9
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 39.9 bits (89), Expect = 5e-04
Identities = 31/118 (26%), Positives = 39/118 (33%)
Frame = +3
Query: 546 PPXPPPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLGXPXPXXXPXXKIXFXXGTXGPXS 725
PP P P PP + + PP P+ P P P +I P
Sbjct: 1032 PPIPVPSTA-----PPVP--IPTSTPPVPKSSSGAPSAPPPVPAPSSEIP---SIPAPSG 1081
Query: 726 XXFXXAPPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPPXG 899
AP P P P PP P +PP P +G P ++ PP P G
Sbjct: 1082 APPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKP-SVAAPPVPVPSG 1138
Score = 32.7 bits (71), Expect = 0.078
Identities = 16/53 (30%), Positives = 18/53 (33%)
Frame = +3
Query: 741 APPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPPXG 899
AP P P P PP P +PP P A P + P P G
Sbjct: 1154 APSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVG 1206
Score = 29.9 bits (64), Expect = 0.55
Identities = 25/106 (23%), Positives = 28/106 (26%), Gaps = 2/106 (1%)
Frame = +3
Query: 516 VPKKXXXXXXPPXPPPXXXXXXXXPPXXRGVXXAPP-PXPQXXXXXLGXPXPXXXPXXKI 692
VP P P PP + APP P P + P P
Sbjct: 1135 VPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Query: 693 XFXXGTXGPXSXXFXXAPPXXXPX-PNPXXTTPPXXLPXXXLPPXP 827
P PP P P P PP +P PP P
Sbjct: 1195 SEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVP 1240
Score = 29.5 bits (63), Expect = 0.72
Identities = 36/144 (25%), Positives = 39/144 (27%), Gaps = 18/144 (12%)
Frame = +3
Query: 516 VPKKXXXXXXPPXPPPXXXXXXXXPPXXRGVXXAPPPX--PQXXXXXLGXPXPXXXPXXK 689
VPK P P P P G P P P + P P P
Sbjct: 1052 VPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAP-PVPKPSVA 1110
Query: 690 IXFXXGTXG--PXSXXFXXAPPXXXPX-----PNPXXTTPPXXLPXXXLP------PXPP 830
+ G P APP P P P PP P P PP
Sbjct: 1111 VPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPP 1170
Query: 831 XAGPXAGXXP---XALXHPPXXPP 893
P +G P A PP PP
Sbjct: 1171 VPAPSSGIPPVPKPAAGVPPVPPP 1194
Score = 28.3 bits (60), Expect = 1.7
Identities = 33/128 (25%), Positives = 34/128 (26%), Gaps = 3/128 (2%)
Frame = +3
Query: 516 VPKKXXXXXXPPXP---PPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLGXPXPXXXPXX 686
VPK P P PP PP P P P + P P
Sbjct: 1123 VPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVP 1182
Query: 687 KIXFXXGTXGPXSXXFXXAPPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXA 866
K P S APP P P PP P P P AG P A
Sbjct: 1183 KPAAGVPPVPPPSE----APPV----PKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTA 1234
Query: 867 LXHPPXXP 890
P P
Sbjct: 1235 KAPPVPAP 1242
Score = 27.9 bits (59), Expect = 2.2
Identities = 32/133 (24%), Positives = 36/133 (27%), Gaps = 8/133 (6%)
Frame = +3
Query: 516 VPKKXXXXXXPPXP---PPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLGXPXPXXXPXX 686
VPK P P PP PP V PP P+ P P P
Sbjct: 1104 VPKPSVAVPPVPAPSGAPPVPKPSVAAPPVP--VPSGAPPVPKPSVAAPPVPAPSGAPP- 1160
Query: 687 KIXFXXGTXGPXSXXFXXAPPXXXPXPN-----PXXTTPPXXLPXXXLPPXPPXAGPXAG 851
+ P PP P P PP P +PP PP +
Sbjct: 1161 -VPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 852 XXPXALXHPPXXP 890
P A P P
Sbjct: 1220 PTPSAGLPPVPVP 1232
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 38.7 bits (86), Expect = 0.001
Identities = 33/118 (27%), Positives = 35/118 (29%), Gaps = 2/118 (1%)
Frame = +3
Query: 546 PPXPPPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLGXPXPXXXPXXKIXFXXGTXGPXS 725
PP PPP P +G APPP P G P P P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQG-RSAPPPPPPRSAPSTGRQPP---PLSSSRAVSNPPAPPP 393
Query: 726 XXFXXAPPXXXPXPNPXXT-TPPXXLPXXXLPPXPPXAGPXA-GXXPXALXHPPXXPP 893
+ P P N T TPP P P PP P A P P PP
Sbjct: 394 AIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPP 451
Score = 32.3 bits (70), Expect = 0.10
Identities = 28/125 (22%), Positives = 32/125 (25%)
Frame = +3
Query: 516 VPKKXXXXXXPPXPPPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLGXPXPXXXPXXKIX 695
+P PP PPP P A P G P P
Sbjct: 351 IPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNAS 410
Query: 696 FXXGTXGPXSXXFXXAPPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXH 875
P + P P P+ + P LPP P A P P A
Sbjct: 411 RTSTPPVPTPPSLPPSAPPSLP-PSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPL 469
Query: 876 PPXXP 890
PP P
Sbjct: 470 PPAAP 474
Score = 31.9 bits (69), Expect = 0.14
Identities = 28/119 (23%), Positives = 32/119 (26%), Gaps = 3/119 (2%)
Frame = +3
Query: 546 PPXPPPXXXXXXXXPPXXRGVXXA---PPPXPQXXXXXLGXPXPXXXPXXKIXFXXGTXG 716
PP PPP PP G + PPP P P P P +
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLP---PQGRSAPPPPPPR 367
Query: 717 PXSXXFXXAPPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPP 893
PP PP +P P PP P + PP PP
Sbjct: 368 SAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTP-PVPTPPSLPP 425
Score = 26.2 bits (55), Expect = 6.7
Identities = 16/51 (31%), Positives = 20/51 (39%)
Frame = +3
Query: 741 APPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPP 893
APP P+ + PP +P P PP P G + PP PP
Sbjct: 229 APPIPPSIPS---SRPPERVPSLSAPAPPPIPPPSNG----TVSSPPNSPP 272
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.31
Identities = 19/57 (33%), Positives = 19/57 (33%)
Frame = +3
Query: 744 PPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPPXGXRGXS 914
P P P P P P PP PP AG P PP PP G S
Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP---PPPPPPAVSAGGS 790
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = +2
Query: 554 PPPXXXXGPXXPPLXXRGXXGSPPXPPXP 640
PPP G PP G G+ P PP P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 26.6 bits (56), Expect = 5.1
Identities = 16/48 (33%), Positives = 17/48 (35%), Gaps = 3/48 (6%)
Frame = +3
Query: 759 PXPNPXXTTP-PXXLPXXXLPPXPPXAGPXAGXXPXAL--XHPPXXPP 893
P P P P P P PP P GP P + PP PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 26.2 bits (55), Expect = 6.7
Identities = 20/63 (31%), Positives = 20/63 (31%), Gaps = 4/63 (6%)
Frame = +3
Query: 516 VPKKXXXXXXPPXPPPXXXXXXXXPPXXRGVXXAPPPXPQXXXXXLG----XPXPXXXPX 683
VP PP PPP PP G PPP P G P P P
Sbjct: 751 VPPPAPIMGGPPPPPP--------PPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPE 802
Query: 684 XKI 692
KI
Sbjct: 803 PKI 805
Score = 25.8 bits (54), Expect = 8.9
Identities = 13/42 (30%), Positives = 15/42 (35%)
Frame = +3
Query: 783 TPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPPXGXRG 908
+PP P +P P P P PP PP G G
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAG 772
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.7
Identities = 16/51 (31%), Positives = 17/51 (33%)
Frame = +3
Query: 741 APPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPP 893
A P P P PP PP PP + P P PP PP
Sbjct: 1684 AHPVSTPPVRPQSAAPPQM--SAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732
Score = 26.2 bits (55), Expect = 6.7
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +3
Query: 546 PPXPPPXXXXXXXXPPXXRGVXXAPPP 626
PP PP PP G APPP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPP 1733
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.5 bits (58), Expect = 2.9
Identities = 15/36 (41%), Positives = 15/36 (41%)
Frame = +3
Query: 741 APPXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXA 848
APP P P PP P LPP P AG A
Sbjct: 1881 APPPPPPMALPK-AGPPSAAPTSALPPAGPPAGATA 1915
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/54 (29%), Positives = 17/54 (31%)
Frame = +3
Query: 747 PXXXPXPNPXXTTPPXXLPXXXLPPXPPXAGPXAGXXPXALXHPPXXPPXGXRG 908
P PN + PP P LPP P P P P P G G
Sbjct: 509 PGAPGMPNLNMSQPPMVPPGMALPPGMPAPFPGYPAVPAMPGIPGATAPPGAPG 562
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,551,314
Number of Sequences: 5004
Number of extensions: 40632
Number of successful extensions: 187
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 483319012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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