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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_C04
         (998 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro...   318   8e-88
SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos...   317   2e-87
SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch...   316   2e-87
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos...    27   3.1  
SPBC18H10.10c |cwc16||complexed with Cdc5 protein Cwf16|Schizosa...    27   3.1  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    27   5.5  
SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p...    26   9.5  
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb...    26   9.5  

>SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal
           protein S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  318 bits (781), Expect = 8e-88
 Identities = 144/196 (73%), Positives = 164/196 (83%)
 Frame = +3

Query: 72  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
           V  I+ QRLIKVDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61  VKAILMQRLIKVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAK 120

Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
           YKLCKVKRV  G K VP+LVTHDGRTIRYPDPLI VND+I+L+  T KI  FIKF++   
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180

Query: 612 CMITGGRNLGRVGTIV 659
            M+TGGRN+GRVGTIV
Sbjct: 181 VMVTGGRNMGRVGTIV 196



 Score = 29.9 bits (64), Expect = 0.59
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +2

Query: 635 LGACGHHRVRERHPGSFDIVHIKDS 709
           +G  G    RE H GSF+I+H+KD+
Sbjct: 189 MGRVGTIVHREHHLGSFEIIHVKDA 213


>SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 262

 Score =  317 bits (778), Expect = 2e-87
 Identities = 143/196 (72%), Positives = 164/196 (83%)
 Frame = +3

Query: 72  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
           V  I+ QRLIKVDGKVRTD T+P GFMDV+S++KT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61  VKAILMQRLIKVDGKVRTDSTFPTGFMDVISVDKTGEHFRLVYDIKGRFTVHRITAEEAK 120

Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
           YKLCKVKRV  G K VP+LVTHDGRTIRYPDPLI VND+I+L+  T KI  FIKF++   
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180

Query: 612 CMITGGRNLGRVGTIV 659
            M+TGGRN+GRVGTIV
Sbjct: 181 VMVTGGRNMGRVGTIV 196



 Score = 29.9 bits (64), Expect = 0.59
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +2

Query: 635 LGACGHHRVRERHPGSFDIVHIKDS 709
           +G  G    RE H GSF+I+H+KD+
Sbjct: 189 MGRVGTIVHREHHLGSFEIIHVKDA 213


>SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  316 bits (777), Expect = 2e-87
 Identities = 143/196 (72%), Positives = 164/196 (83%)
 Frame = +3

Query: 72  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
           V  I+ QRLI+VDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61  VKAILMQRLIQVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAK 120

Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
           YKLCKVKRV  G K VP+LVTHDGRTIRYPDPLI VND+I+L+  T KI  FIKF++   
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180

Query: 612 CMITGGRNLGRVGTIV 659
            M+TGGRN+GRVGTIV
Sbjct: 181 VMVTGGRNMGRVGTIV 196



 Score = 29.9 bits (64), Expect = 0.59
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = +2

Query: 635 LGACGHHRVRERHPGSFDIVHIKDS 709
           +G  G    RE H GSF+I+H+KD+
Sbjct: 189 MGRVGTIVHREHHLGSFEIIHVKDA 213


>SPCC1450.11c |cek1||serine/threonine protein kinase
           Cek1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1338

 Score = 27.5 bits (58), Expect = 3.1
 Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
 Frame = -1

Query: 326 SSRISRVSPNFPIN--LYEALFH-NFQDFVSGQSILQTIPQENHQGQALAQLVGT 171
           SS+   + P+ P++   +E  F  NFQ      S L  IP++   G ++A+++GT
Sbjct: 27  SSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGT 81


>SPBC18H10.10c |cwc16||complexed with Cdc5 protein
           Cwf16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 294

 Score = 27.5 bits (58), Expect = 3.1
 Identities = 20/81 (24%), Positives = 33/81 (40%), Gaps = 1/81 (1%)
 Frame = +3

Query: 267 KQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCK 446
           K++  K+   +R +  +P    +  +I +    F  +    G +   +I     K  LC 
Sbjct: 13  KRKFDKLRNVIRFEMPFPVWCNNCENIIQQGTRFNAVKKEIGSYYTTKIWSFSLKCHLCS 72

Query: 447 VK-RVATGPKNVPYLVTHDGR 506
               V T PKN  Y+V   GR
Sbjct: 73  NPIDVHTDPKNTEYIVASGGR 93


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
 Frame = +3

Query: 333  DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYL-VTHDGRT 509
            +V S+EK N++F + Y+V  +     +   +   K+  ++ ++   KNVP + V H    
Sbjct: 1718 NVSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQIISFFLKNVPEITVQHQTEM 1777

Query: 510  IR 515
            ++
Sbjct: 1778 LK 1779


>SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 390

 Score = 25.8 bits (54), Expect = 9.5
 Identities = 9/15 (60%), Positives = 10/15 (66%)
 Frame = +2

Query: 500 WPHHPLPRPTYHSQR 544
           WPHH LP   + SQR
Sbjct: 265 WPHHNLPSQFFTSQR 279


>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1428

 Score = 25.8 bits (54), Expect = 9.5
 Identities = 8/14 (57%), Positives = 11/14 (78%)
 Frame = +2

Query: 536 SQRFHPVRHXNYED 577
           +++ HPVRH  YED
Sbjct: 6   NEKIHPVRHSKYED 19


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,650,131
Number of Sequences: 5004
Number of extensions: 75016
Number of successful extensions: 186
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 517271174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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