Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_C04
(998 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 302 2e-82
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 302 3e-82
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 302 3e-82
At4g38440.1 68417.m05432 expressed protein 31 1.2
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 1.2
At1g29760.1 68414.m03639 expressed protein 31 1.2
At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar... 31 1.6
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 4.8
At3g22310.1 68416.m02818 DEAD box RNA helicase, putative (RH9) s... 29 6.4
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 8.4
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 8.4
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 8.4
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 302 bits (742), Expect = 2e-82
Identities = 139/195 (71%), Positives = 161/195 (82%)
Frame = +3
Query: 72 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
V+ I+ QR I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAK 120
Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
+KLCKV+ + G K +PYL T+DGRTIRYPDPLI ND+I+LD KI++FIKF+ GN+
Sbjct: 121 FKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNV 180
Query: 612 CMITGGRNLGRVGTI 656
M+TGGRN GRVG I
Sbjct: 181 VMVTGGRNRGRVGVI 195
Score = 34.7 bits (76), Expect = 0.097
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +2
Query: 638 GACGHHRVRERHPGSFDIVHIKDSTG 715
G G + RE+H GSF+ +HI+DSTG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTG 215
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 302 bits (741), Expect = 3e-82
Identities = 139/195 (71%), Positives = 161/195 (82%)
Frame = +3
Query: 72 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
V+ I+ QR I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAK 120
Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
+KLCKV+ + G K +PYL T+DGRTIRYPDPLI ND+I+LD KI++FIKF+ GN+
Sbjct: 121 FKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNV 180
Query: 612 CMITGGRNLGRVGTI 656
M+TGGRN GRVG I
Sbjct: 181 VMVTGGRNRGRVGVI 195
Score = 34.7 bits (76), Expect = 0.097
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +2
Query: 638 GACGHHRVRERHPGSFDIVHIKDSTG 715
G G + RE+H GSF+ +HI+DSTG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTG 215
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 302 bits (741), Expect = 3e-82
Identities = 139/195 (71%), Positives = 161/195 (82%)
Frame = +3
Query: 72 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 251
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60
Query: 252 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 431
V+ I+ QR I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK
Sbjct: 61 VISILMQRHIQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAK 120
Query: 432 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLITVNDSIQLDXATTKIMDFIKFESGNL 611
+KLCKV+ + G K +PYL T+DGRTIRYPDPLI ND+I+LD KI++FIKF+ GN+
Sbjct: 121 FKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNV 180
Query: 612 CMITGGRNLGRVGTI 656
M+TGGRN GRVG I
Sbjct: 181 VMVTGGRNRGRVGVI 195
Score = 34.7 bits (76), Expect = 0.097
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +2
Query: 638 GACGHHRVRERHPGSFDIVHIKDSTG 715
G G + RE+H GSF+ +HI+DSTG
Sbjct: 190 GRVGVIKNREKHKGSFETIHIQDSTG 215
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 31.1 bits (67), Expect = 1.2
Identities = 25/104 (24%), Positives = 45/104 (43%), Gaps = 4/104 (3%)
Frame = +3
Query: 99 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL----VIFLRNRLKYALTGNEVLKIV 266
+R+ P W L + V++ + STGP + E L + V FL + G+ +V
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELLEVAKAGVFFLAGLESSSGFGSLPSPVV 1199
Query: 267 KQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRF 398
L+ K T MD++ + T L+ + ++ G+F
Sbjct: 1200 SVPLV---WKFHALSTVLLVGMDIIEDKNTRNLYNYLQELYGQF 1240
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 1.2
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 196 KHSRNLWGPVDGLGAYTPPSLSNIHAL 116
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 1.2
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 307 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 215
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar to
RNA helicases GI:3775995, GI:3775987 from [Arabidopsis
thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
helicase, PF00271: Helicase conserved C-terminal domain
Length = 616
Score = 30.7 bits (66), Expect = 1.6
Identities = 18/65 (27%), Positives = 32/65 (49%)
Frame = -2
Query: 730 VAXVXXRGVLDVHNVEGAGMSLADTMVPTRPKLRPPVIIHKFPDSNLMKSIIFVVAMSNW 551
V + RG L++ V+ + AD M+ +I+ K P+ +S++F M +W
Sbjct: 237 VIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILEKLPEKR--QSMMFSATMPSW 294
Query: 550 MESLT 536
+ SLT
Sbjct: 295 IRSLT 299
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 4.8
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 286 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 170
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At3g22310.1 68416.m02818 DEAD box RNA helicase, putative (RH9)
similar to RNA helicases GI:3775995, GI:3775987
[Arabidopsis thaliana]; contains Pfam profiles PF00270:
DEAD/DEAH box helicase, PF00271: Helicase conserved
C-terminal domain
Length = 610
Score = 28.7 bits (61), Expect = 6.4
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = -2
Query: 712 RGVLDVHNVEGAGMSLADTMVPTRPKLRPPVIIHKFPDSNLMKSIIFVVAMSNWMESLT 536
RG L++ V+ + AD M+ +I+ K P +S++F M +W+ SLT
Sbjct: 255 RGALNLSEVQFVVLDEADQMLQVGFAEDVEIILQKLPAKR--QSMMFSATMPSWIRSLT 311
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 8.4
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 219 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 353
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 8.4
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 219 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 353
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 8.4
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +3
Query: 219 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 353
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,067,532
Number of Sequences: 28952
Number of extensions: 442682
Number of successful extensions: 1290
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1289
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2441087448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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