BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_B24 (1067 letters) Database: fruitfly 53,049 sequences; 24,988,368 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY973546-1|AAX84047.1| 962|Drosophila melanogaster midlife cris... 71 2e-12 AY973545-1|AAX84046.1| 962|Drosophila melanogaster midlife cris... 71 2e-12 AE013599-1094|AAM68758.1| 598|Drosophila melanogaster CG11763-P... 71 2e-12 AE013599-1093|AAF58795.2| 960|Drosophila melanogaster CG11763-P... 71 2e-12 AE013599-1092|ABC66064.1| 962|Drosophila melanogaster CG11763-P... 71 2e-12 AY052091-1|AAK93515.1| 445|Drosophila melanogaster SD04057p pro... 48 3e-05 AY119485-1|AAM50139.1| 846|Drosophila melanogaster GH07623p pro... 34 0.38 AE014297-4203|AAF56763.2| 1109|Drosophila melanogaster CG5514-PA... 34 0.38 AE014297-4202|AAN14134.1| 1150|Drosophila melanogaster CG5514-PB... 34 0.38 BT025908-1|ABG02152.1| 400|Drosophila melanogaster IP05642p pro... 29 8.2 AE014134-2537|AAX52666.1| 867|Drosophila melanogaster CG33515-P... 29 8.2 >AY973546-1|AAX84047.1| 962|Drosophila melanogaster midlife crisis protein. Length = 962 Score = 71.3 bits (167), Expect = 2e-12 Identities = 35/54 (64%), Positives = 39/54 (72%), Gaps = 1/54 (1%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXGXGXTHRDAASVRATHPIPAA 320 +NE ETPLPR WS DK IGLSQNNLRV Y G G H DAASVR +PIP++ Sbjct: 411 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKGVGKQHSDAASVRTAYPIPSS 464 Score = 40.7 bits (91), Expect = 0.003 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 313 PPPCGLYYFEVRIVSKGRXG*WG 381 P CGLYYFEVRI+SKGR G G Sbjct: 462 PSSCGLYYFEVRIISKGRNGYMG 484 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 392 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 421 >AY973545-1|AAX84046.1| 962|Drosophila melanogaster midlife crisis protein. Length = 962 Score = 71.3 bits (167), Expect = 2e-12 Identities = 35/54 (64%), Positives = 39/54 (72%), Gaps = 1/54 (1%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXGXGXTHRDAASVRATHPIPAA 320 +NE ETPLPR WS DK IGLSQNNLRV Y G G H DAASVR +PIP++ Sbjct: 411 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKGVGKQHSDAASVRTAYPIPSS 464 Score = 40.7 bits (91), Expect = 0.003 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 313 PPPCGLYYFEVRIVSKGRXG*WG 381 P CGLYYFEVRI+SKGR G G Sbjct: 462 PSSCGLYYFEVRIISKGRNGYMG 484 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 392 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 421 >AE013599-1094|AAM68758.1| 598|Drosophila melanogaster CG11763-PC, isoform C protein. Length = 598 Score = 71.3 bits (167), Expect = 2e-12 Identities = 35/54 (64%), Positives = 39/54 (72%), Gaps = 1/54 (1%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXGXGXTHRDAASVRATHPIPAA 320 +NE ETPLPR WS DK IGLSQNNLRV Y G G H DAASVR +PIP++ Sbjct: 49 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKGVGKQHSDAASVRTAYPIPSS 102 Score = 40.7 bits (91), Expect = 0.003 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 313 PPPCGLYYFEVRIVSKGRXG*WG 381 P CGLYYFEVRI+SKGR G G Sbjct: 100 PSSCGLYYFEVRIISKGRNGYMG 122 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 30 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 59 >AE013599-1093|AAF58795.2| 960|Drosophila melanogaster CG11763-PA, isoform A protein. Length = 960 Score = 71.3 bits (167), Expect = 2e-12 Identities = 35/54 (64%), Positives = 39/54 (72%), Gaps = 1/54 (1%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXGXGXTHRDAASVRATHPIPAA 320 +NE ETPLPR WS DK IGLSQNNLRV Y G G H DAASVR +PIP++ Sbjct: 411 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKGVGKQHSDAASVRTAYPIPSS 464 Score = 40.7 bits (91), Expect = 0.003 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 313 PPPCGLYYFEVRIVSKGRXG*WG 381 P CGLYYFEVRI+SKGR G G Sbjct: 462 PSSCGLYYFEVRIISKGRNGYMG 484 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 392 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 421 >AE013599-1092|ABC66064.1| 962|Drosophila melanogaster CG11763-PD, isoform D protein. Length = 962 Score = 71.3 bits (167), Expect = 2e-12 Identities = 35/54 (64%), Positives = 39/54 (72%), Gaps = 1/54 (1%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXGXGXTHRDAASVRATHPIPAA 320 +NE ETPLPR WS DK IGLSQNNLRV Y G G H DAASVR +PIP++ Sbjct: 411 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKGVGKQHSDAASVRTAYPIPSS 464 Score = 40.7 bits (91), Expect = 0.003 Identities = 17/23 (73%), Positives = 18/23 (78%) Frame = +1 Query: 313 PPPCGLYYFEVRIVSKGRXG*WG 381 P CGLYYFEVRI+SKGR G G Sbjct: 462 PSSCGLYYFEVRIISKGRNGYMG 484 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 392 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 421 >AY052091-1|AAK93515.1| 445|Drosophila melanogaster SD04057p protein. Length = 445 Score = 47.6 bits (108), Expect = 3e-05 Identities = 24/34 (70%), Positives = 25/34 (73%), Gaps = 1/34 (2%) Frame = +3 Query: 162 MNE-ETPLPRAWSTKDKFS*IGLSQNNLRVHYXG 260 +NE ETPLPR WS DK IGLSQNNLRV Y G Sbjct: 411 VNESETPLPRCWSPHDKCLSIGLSQNNLRVTYKG 444 Score = 32.3 bits (70), Expect = 1.2 Identities = 15/30 (50%), Positives = 16/30 (53%) Frame = +2 Query: 101 SQNQVQSDTVDRLKLLYPNVNERRDSFTPC 190 S N Q VD L+LLYPNVNE C Sbjct: 392 SSNSPQHQGVDPLRLLYPNVNESETPLPRC 421 >AY119485-1|AAM50139.1| 846|Drosophila melanogaster GH07623p protein. Length = 846 Score = 33.9 bits (74), Expect = 0.38 Identities = 19/65 (29%), Positives = 23/65 (35%) Frame = +1 Query: 652 PXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXFXXCPXLPSXXG 831 P P PP+ V PP P PP T+ P P + P P P+ Sbjct: 141 PPPAPPTLVPPPPPAPPTIKPPPPPAPPTVEPPPPPPPAPPTVEPP---PPPPPAPTKVE 197 Query: 832 PPXXP 846 PP P Sbjct: 198 PPPPP 202 Score = 31.9 bits (69), Expect = 1.5 Identities = 23/76 (30%), Positives = 24/76 (31%) Frame = +1 Query: 619 PXXXLSSTXXRPXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXF 798 P S P P PP PP P PP TI PPP A P+ Sbjct: 97 PPRPASPKVEPPPPAPPGVESPPGPQPPASPRFDPPPPHTI-----EPPPPPA--PPTLV 149 Query: 799 XXCPXLPSXXGPPXXP 846 P P PP P Sbjct: 150 PPPPPAPPTIKPPPPP 165 >AE014297-4203|AAF56763.2| 1109|Drosophila melanogaster CG5514-PA, isoform A protein. Length = 1109 Score = 33.9 bits (74), Expect = 0.38 Identities = 19/65 (29%), Positives = 23/65 (35%) Frame = +1 Query: 652 PXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXFXXCPXLPSXXG 831 P P PP+ V PP P PP T+ P P + P P P+ Sbjct: 404 PPPAPPTLVPPPPPAPPTIKPPPPPAPPTVEPPPPPPPAPPTVEPP---PPPPPAPTKVE 460 Query: 832 PPXXP 846 PP P Sbjct: 461 PPPPP 465 Score = 31.9 bits (69), Expect = 1.5 Identities = 23/76 (30%), Positives = 24/76 (31%) Frame = +1 Query: 619 PXXXLSSTXXRPXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXF 798 P S P P PP PP P PP TI PPP A P+ Sbjct: 360 PPRPASPKVEPPPPAPPGVESPPGPQPPASPRFDPPPPHTI-----EPPPPPA--PPTLV 412 Query: 799 XXCPXLPSXXGPPXXP 846 P P PP P Sbjct: 413 PPPPPAPPTIKPPPPP 428 >AE014297-4202|AAN14134.1| 1150|Drosophila melanogaster CG5514-PB, isoform B protein. Length = 1150 Score = 33.9 bits (74), Expect = 0.38 Identities = 19/65 (29%), Positives = 23/65 (35%) Frame = +1 Query: 652 PXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXFXXCPXLPSXXG 831 P P PP+ V PP P PP T+ P P + P P P+ Sbjct: 404 PPPAPPTLVPPPPPAPPTIKPPPPPAPPTVEPPPPPPPAPPTVEPP---PPPPPAPTKVE 460 Query: 832 PPXXP 846 PP P Sbjct: 461 PPPPP 465 Score = 31.9 bits (69), Expect = 1.5 Identities = 23/76 (30%), Positives = 24/76 (31%) Frame = +1 Query: 619 PXXXLSSTXXRPXPNPPSXVXPPXPXPPXXXXXXXXXXGTITHGXXNXPPPTAFQXPSXF 798 P S P P PP PP P PP TI PPP A P+ Sbjct: 360 PPRPASPKVEPPPPAPPGVESPPGPQPPASPRFDPPPPHTI-----EPPPPPA--PPTLV 412 Query: 799 XXCPXLPSXXGPPXXP 846 P P PP P Sbjct: 413 PPPPPAPPTIKPPPPP 428 >BT025908-1|ABG02152.1| 400|Drosophila melanogaster IP05642p protein. Length = 400 Score = 29.5 bits (63), Expect = 8.2 Identities = 17/46 (36%), Positives = 29/46 (63%), Gaps = 2/46 (4%) Frame = +2 Query: 44 RILGNHPW--LR*VVDGATMDSQNQVQSDTVDRLKLLYPNVNERRD 175 R++G P +R +V+G +D+QN+ S V +LL+P+V +R D Sbjct: 103 RVVGGRPQPQVRWLVNGLLVDNQNEHNSGDVIENRLLWPSV-QRND 147 >AE014134-2537|AAX52666.1| 867|Drosophila melanogaster CG33515-PA protein. Length = 867 Score = 29.5 bits (63), Expect = 8.2 Identities = 17/46 (36%), Positives = 29/46 (63%), Gaps = 2/46 (4%) Frame = +2 Query: 44 RILGNHPW--LR*VVDGATMDSQNQVQSDTVDRLKLLYPNVNERRD 175 R++G P +R +V+G +D+QN+ S V +LL+P+V +R D Sbjct: 207 RVVGGRPQPQVRWLVNGLLVDNQNEHNSGDVIENRLLWPSV-QRND 251 Database: fruitfly Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 24,988,368 Number of sequences in database: 53,049 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 32,345,410 Number of Sequences: 53049 Number of extensions: 605006 Number of successful extensions: 3132 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 1617 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 2762 length of database: 24,988,368 effective HSP length: 86 effective length of database: 20,426,154 effective search space used: 5494635426 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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