BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_B23
(1060 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 39 0.001
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.4
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 3.4
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.9
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 7.8
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 39.1 bits (87), Expect = 0.001
Identities = 24/65 (36%), Positives = 27/65 (41%)
Frame = +2
Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXPTXPX 694
APP P+P T PP P TPP P + GA PP AP PSI P
Sbjct: 1031 APPIPVPSTA----PPVPIPTSTPPVPKSSSGAPSAPPPVP--APSSEIPSIPAPSGAPP 1084
Query: 695 GXTPT 709
P+
Sbjct: 1085 VPAPS 1089
Score = 33.1 bits (72), Expect = 0.068
Identities = 21/64 (32%), Positives = 22/64 (34%)
Frame = +2
Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXPTXPX 694
APP P P A P G PP P G PP AP PS+ P P
Sbjct: 1158 APPVPKPSVAAPPVPAPSSG--IPPVPKPAAGVPPVPPP--SEAPPVPKPSVGVPPVPPP 1213
Query: 695 GXTP 706
P
Sbjct: 1214 STAP 1217
Score = 28.3 bits (60), Expect = 1.9
Identities = 19/56 (33%), Positives = 20/56 (35%)
Frame = +2
Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXP 682
APP P P A PP P PP P A P AP PS+ P
Sbjct: 1120 APPVPKPSVAA---PPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAPP 1170
Score = 27.5 bits (58), Expect = 3.4
Identities = 19/55 (34%), Positives = 19/55 (34%)
Frame = +2
Query: 518 PPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXP 682
PP P P A PP P PP P G PP AP PS P
Sbjct: 1179 PPVPKP---AAGVPPVPPPSEAPPVPKPSVGVPPVPPP--STAPPVPTPSAGLPP 1228
Score = 27.5 bits (58), Expect = 3.4
Identities = 18/51 (35%), Positives = 20/51 (39%)
Frame = +2
Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPS 667
APP P P PP P PP P+ G VP +AP PS
Sbjct: 1197 APPVPKPSVGV---PPVPPPSTAPPVPTPSAG-LPPVPVPTAKAPPVPAPS 1243
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 3.4
Identities = 17/56 (30%), Positives = 20/56 (35%), Gaps = 2/56 (3%)
Frame = +3
Query: 558 PRXPXAXXRPPXLQPXXAXXWXXPPXGXAPPXREIPQX--SXXPPHQXAXPXQXPP 719
P P + RPP P P P + P S PP Q A P + PP
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185
Score = 27.1 bits (57), Expect = 4.5
Identities = 22/64 (34%), Positives = 24/64 (37%), Gaps = 7/64 (10%)
Frame = +2
Query: 515 APPHPI----PGTXARKXP--PXPXGXXTPPXPSTXXGAXXXV-PPXXXRAPXKRNPSIX 673
APP P P T A P P P PP PS + PP AP K PS
Sbjct: 129 APPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAP 188
Query: 674 QXPT 685
P+
Sbjct: 189 SLPS 192
Score = 26.6 bits (56), Expect = 5.9
Identities = 15/42 (35%), Positives = 15/42 (35%)
Frame = +2
Query: 869 PPPXK*XXXPXRXPXTPXPPXPXXPSXPPXXSXXPXPXPXQL 994
P P P P P P P S PP S P P P L
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAP-PIPSSL 171
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 3.4
Identities = 15/48 (31%), Positives = 16/48 (33%)
Frame = +2
Query: 506 GNTAPPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAP 649
G PP P PG PP P PP P+ G P P
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPP-----PPPPAVSAGGSRYYAPAPQAEP 801
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.9
Identities = 13/45 (28%), Positives = 17/45 (37%)
Frame = +2
Query: 512 TAPPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRA 646
T PP P+ PP P G + P P + VP R+
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.2 bits (55), Expect = 7.8
Identities = 20/56 (35%), Positives = 21/56 (37%), Gaps = 6/56 (10%)
Frame = +2
Query: 482 PXSXSXXGGNT-APPHPIPGTXARKXPPXPXGXXT-----PPXPSTXXGAXXXVPP 631
P S S N APP IPG A PP T P PS A +PP
Sbjct: 378 PLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,575,289
Number of Sequences: 5004
Number of extensions: 16678
Number of successful extensions: 95
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 557214894
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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