BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_B23 (1060 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 39 0.001 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.4 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 3.4 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.9 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 7.8 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 39.1 bits (87), Expect = 0.001 Identities = 24/65 (36%), Positives = 27/65 (41%) Frame = +2 Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXPTXPX 694 APP P+P T PP P TPP P + GA PP AP PSI P Sbjct: 1031 APPIPVPSTA----PPVPIPTSTPPVPKSSSGAPSAPPPVP--APSSEIPSIPAPSGAPP 1084 Query: 695 GXTPT 709 P+ Sbjct: 1085 VPAPS 1089 Score = 33.1 bits (72), Expect = 0.068 Identities = 21/64 (32%), Positives = 22/64 (34%) Frame = +2 Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXPTXPX 694 APP P P A P G PP P G PP AP PS+ P P Sbjct: 1158 APPVPKPSVAAPPVPAPSSG--IPPVPKPAAGVPPVPPP--SEAPPVPKPSVGVPPVPPP 1213 Query: 695 GXTP 706 P Sbjct: 1214 STAP 1217 Score = 28.3 bits (60), Expect = 1.9 Identities = 19/56 (33%), Positives = 20/56 (35%) Frame = +2 Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXP 682 APP P P A PP P PP P A P AP PS+ P Sbjct: 1120 APPVPKPSVAA---PPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAPP 1170 Score = 27.5 bits (58), Expect = 3.4 Identities = 19/55 (34%), Positives = 19/55 (34%) Frame = +2 Query: 518 PPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPSIXQXP 682 PP P P A PP P PP P G PP AP PS P Sbjct: 1179 PPVPKP---AAGVPPVPPPSEAPPVPKPSVGVPPVPPP--STAPPVPTPSAGLPP 1228 Score = 27.5 bits (58), Expect = 3.4 Identities = 18/51 (35%), Positives = 20/51 (39%) Frame = +2 Query: 515 APPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAPXKRNPS 667 APP P P PP P PP P+ G VP +AP PS Sbjct: 1197 APPVPKPSVGV---PPVPPPSTAPPVPTPSAG-LPPVPVPTAKAPPVPAPS 1243 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 27.5 bits (58), Expect = 3.4 Identities = 17/56 (30%), Positives = 20/56 (35%), Gaps = 2/56 (3%) Frame = +3 Query: 558 PRXPXAXXRPPXLQPXXAXXWXXPPXGXAPPXREIPQX--SXXPPHQXAXPXQXPP 719 P P + RPP P P P + P S PP Q A P + PP Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185 Score = 27.1 bits (57), Expect = 4.5 Identities = 22/64 (34%), Positives = 24/64 (37%), Gaps = 7/64 (10%) Frame = +2 Query: 515 APPHPI----PGTXARKXP--PXPXGXXTPPXPSTXXGAXXXV-PPXXXRAPXKRNPSIX 673 APP P P T A P P P PP PS + PP AP K PS Sbjct: 129 APPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAP 188 Query: 674 QXPT 685 P+ Sbjct: 189 SLPS 192 Score = 26.6 bits (56), Expect = 5.9 Identities = 15/42 (35%), Positives = 15/42 (35%) Frame = +2 Query: 869 PPPXK*XXXPXRXPXTPXPPXPXXPSXPPXXSXXPXPXPXQL 994 P P P P P P P S PP S P P P L Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAP-PIPSSL 171 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.5 bits (58), Expect = 3.4 Identities = 15/48 (31%), Positives = 16/48 (33%) Frame = +2 Query: 506 GNTAPPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRAP 649 G PP P PG PP P PP P+ G P P Sbjct: 759 GGPPPPPPPPGVAGAGPPPPP-----PPPPAVSAGGSRYYAPAPQAEP 801 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 26.6 bits (56), Expect = 5.9 Identities = 13/45 (28%), Positives = 17/45 (37%) Frame = +2 Query: 512 TAPPHPIPGTXARKXPPXPXGXXTPPXPSTXXGAXXXVPPXXXRA 646 T PP P+ PP P G + P P + VP R+ Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 26.2 bits (55), Expect = 7.8 Identities = 20/56 (35%), Positives = 21/56 (37%), Gaps = 6/56 (10%) Frame = +2 Query: 482 PXSXSXXGGNT-APPHPIPGTXARKXPPXPXGXXT-----PPXPSTXXGAXXXVPP 631 P S S N APP IPG A PP T P PS A +PP Sbjct: 378 PLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,575,289 Number of Sequences: 5004 Number of extensions: 16678 Number of successful extensions: 95 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 89 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 557214894 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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