BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_B21
(930 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycos... 44 3e-05
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.71
SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi... 29 1.2
SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|c... 28 2.2
SPBC947.06c |||spermidine family transporter |Schizosaccharomyce... 26 6.6
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 8.7
>SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein
glycosyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 44.0 bits (99), Expect = 3e-05
Identities = 25/127 (19%), Positives = 56/127 (44%), Gaps = 2/127 (1%)
Frame = +1
Query: 190 NVDRTVDISTQLVKVVSKIVLENSGKSPVKHFLFAVEEAAKNNL--AFIGAKDSNSKDLR 363
N RT+D+ + K ++ +++ N G P + + + + ++ + K+ K
Sbjct: 26 NAIRTIDLRSTYTKTLTSLIIVNEGDEPQNTYRYFQQTSPGESVLSLSVSIKEEEKKGTN 85
Query: 364 LAETTVKGYDYVKFWRVELKEPLNAGATVNVVVETVFTKALQPYPTAITQQEDXLVKYVG 543
+ YD + L+ P+ G + ++++ ++P P A+ Q E+ + Y+
Sbjct: 86 PIKVNNNVYD------IPLQPPVKPGESRTLLIQAGLDGGVRPLPAAVDQDEEQYLVYLT 139
Query: 544 NLYTYSP 564
LY SP
Sbjct: 140 TLYLDSP 146
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.5 bits (63), Expect = 0.71
Identities = 27/110 (24%), Positives = 49/110 (44%), Gaps = 5/110 (4%)
Frame = +1
Query: 205 VDISTQLVKVVSKIVLENSGKSPVKH-----FLFAVEEAAKNNLAFIGAKDSNSKDLRLA 369
VD+ +QL++++ KIVL+ S S + FL A++ + +I D D +A
Sbjct: 994 VDLPSQLIELLEKIVLQPSSFSENANLQNLLFLTAIKADKSRVMEYIDKLDKYDVD-EIA 1052
Query: 370 ETTVKGYDYVKFWRVELKEPLNAGATVNVVVETVFTKALQPYPTAITQQE 519
E ++ Y + +R+ + A +V + V Q Y + Q E
Sbjct: 1053 EIAIENGLYEEAFRIYKIHNKHEQAMKVLVEDIVSLDRAQDYAETVEQPE 1102
>SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein
Urb1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1568
Score = 28.7 bits (61), Expect = 1.2
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +1
Query: 124 CFLTIQTLSASVDTISSDI-KLKNVDRTVDISTQLVKVVSKIVL 252
CFLT+ TL AS + S + ++K + + D +LV +S +L
Sbjct: 369 CFLTVNTLLASFKRLESVLDEIKRSELSEDNKVELVSKISDAIL 412
>SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 958
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 281 CFTGDLPLFSKTIFDTTFTSWVD 213
C G+LP FSKT ++ T + +D
Sbjct: 884 CVVGELPFFSKTCYNATVVAELD 906
>SPBC947.06c |||spermidine family transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 498
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -2
Query: 305 ASSTAKRKCFTGDLPLFSKTIFDTTFTSW 219
A++T R F G PLFS+ +F W
Sbjct: 433 AANTFTRSIFAGTFPLFSEIMFKKLGVHW 461
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 25.8 bits (54), Expect = 8.7
Identities = 17/34 (50%), Positives = 19/34 (55%)
Frame = +1
Query: 169 SSDIKLKNVDRTVDISTQLVKVVSKIVLENSGKS 270
SSDI N+D T DISTQ S + NSG S
Sbjct: 1303 SSDIPKANIDFTNDISTQNTFPASPV--SNSGFS 1334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,027,565
Number of Sequences: 5004
Number of extensions: 59879
Number of successful extensions: 184
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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