Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_B05
(958 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 35 0.015
SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor |Schizosacc... 31 0.18
SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 30 0.42
SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 27 3.0
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.0
SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr ... 27 5.2
SPAC17G6.07c |||SNARE Slt1 |Schizosaccharomyces pombe|chr 1|||Ma... 27 5.2
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 27 5.2
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 9.0
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 9.0
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 35.1 bits (77), Expect = 0.015
Identities = 30/80 (37%), Positives = 39/80 (48%)
Frame = +2
Query: 137 MPTKPQSPTPNSKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*TAW 316
+PT S TP S TTA+S T PL N +T T A S+ +S NS TT +A
Sbjct: 415 LPTSSVSSTPLSSANSTTATSASST--PLSSVNSTTAT-SASSTPLSSVNS--TTATSAS 469
Query: 317 STPTSYGSKAPKISSGSASL 376
STP + + S+ S L
Sbjct: 470 STPLTSVNSTTATSASSTPL 489
Score = 33.1 bits (72), Expect = 0.060
Identities = 31/102 (30%), Positives = 48/102 (47%), Gaps = 4/102 (3%)
Frame = +2
Query: 83 AKDENRSSYSCVFSWRLCMPTKPQSPTPNSK---TIFTTASSLPITTIPLKRANRSTRTR 253
A + +S S ++ +P+ S TP S T T+ASS P+T++ ST T
Sbjct: 264 ATSASATSSSAQYNTSSLLPSSTPSSTPLSSANSTTATSASSTPLTSV------NSTTTT 317
Query: 254 RAKSSQMS*TNSYETTR*TAW-STPTSYGSKAPKISSGSASL 376
A S+ +S +S +T T+ STP S + S+ S L
Sbjct: 318 SASSTPLSSVSSANSTTATSTSSTPLSSVNSTTATSASSTPL 359
Score = 29.5 bits (63), Expect = 0.73
Identities = 21/67 (31%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = +2
Query: 170 SKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*TAWS-TPTSYGSKA 346
+ T T+ASS P+T++ A ++ T + S T++ T +A S T TS S A
Sbjct: 347 NSTTATSASSTPLTSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTA 406
Query: 347 PKISSGS 367
P ++ S
Sbjct: 407 PSYNTSS 413
Score = 29.5 bits (63), Expect = 0.73
Identities = 21/67 (31%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = +2
Query: 170 SKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*TAWS-TPTSYGSKA 346
+ T T+ASS P+T++ A ++ T + S T++ T +A S T TS S A
Sbjct: 461 NSTTATSASSTPLTSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTA 520
Query: 347 PKISSGS 367
P ++ S
Sbjct: 521 PSYNTSS 527
Score = 26.2 bits (55), Expect = 6.8
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
Frame = +2
Query: 161 TPNSKTIFTTASSLPITTIPLKRAN--RSTRTRRAKSSQMS*TNSYETTR*TAWSTPTSY 334
T + T T+ASS P+T++ A ST A S+ + +S + T+ PTS
Sbjct: 360 TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTSSVLPTSS 419
Query: 335 GSKAPKISSGSAS 373
S P S+ S +
Sbjct: 420 VSSTPLSSANSTT 432
Score = 26.2 bits (55), Expect = 6.8
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
Frame = +2
Query: 161 TPNSKTIFTTASSLPITTIPLKRAN--RSTRTRRAKSSQMS*TNSYETTR*TAWSTPTSY 334
T + T T+ASS P+T++ A ST A S+ + +S + T+ PTS
Sbjct: 474 TSVNSTTATSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTSSVLPTSS 533
Query: 335 GSKAPKISSGSAS 373
S P S+ S +
Sbjct: 534 VSSTPLSSANSTT 546
>SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 539
Score = 31.5 bits (68), Expect = 0.18
Identities = 27/123 (21%), Positives = 47/123 (38%)
Frame = +2
Query: 347 PKISSGSASLFEFRLIFAENNIKLMYKRDGLALTLDDENSNDGRLAYGDGKDKTSPKVSW 526
P G+ S + +N+ +G T +DG L Y D + +T+
Sbjct: 164 PSKVEGALSHLRVTKVICSDNLTAAITDNGCCFTWGTFRCSDGVLGYSDSQKRTAEPTQM 223
Query: 527 KFVPLCEXTTVSYFKIVNTXP*SVFDVGXFQQPRTIXXGLRGPQRPRIXAQWTXQPA*VR 706
+ +C+ T + I T V+ G QQ G R +R R+ T QP ++
Sbjct: 224 RLPEICQLATGTDHIIALTTTGKVYTWGNGQQ---FQLGRRMLERRRLQG-LTPQPLALK 279
Query: 707 HXV 715
+ +
Sbjct: 280 NII 282
>SPCC576.13 |swc5||chromatin remodeling complex subunit
Swc5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 215
Score = 30.3 bits (65), Expect = 0.42
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +1
Query: 223 EKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWLQGSK--DIVRECFPV*VQTYI 396
+K I + +KS + ++ K+++ NK+N +E A Q W + K DI E Y+
Sbjct: 132 KKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKEQDIQDELRIANKDGYV 191
Query: 397 RRKQH*ADVQA 429
R++ A +A
Sbjct: 192 ERQEFLAKTRA 202
>SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 749
Score = 27.5 bits (58), Expect = 3.0
Identities = 27/82 (32%), Positives = 43/82 (52%), Gaps = 3/82 (3%)
Frame = +2
Query: 143 TKPQSPTPNSKT--IFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*TAW 316
+KP S +PN T IFT+ +SL ++ + R S + R+ S + S T + +
Sbjct: 626 SKPSSISPNKATTNIFTSQASL--SSQGVARETSSASSYRSYSQRAS-TPAVTAKSSFHY 682
Query: 317 STPTSYGSKAPKIS-SGSASLF 379
+TPTS S ++ S SASL+
Sbjct: 683 ATPTSGLSNFNSVTPSSSASLY 704
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 3.0
Identities = 22/74 (29%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = +2
Query: 155 SPTPNSKTIFT-TASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSYETTR*TAWSTPTS 331
S +P+S + T T+SSL ++IP ++ S+ + SS S T S ++ + S+ +S
Sbjct: 219 SSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSS 278
Query: 332 YGSKAPKISSGSAS 373
S SS +S
Sbjct: 279 SSSSPTSTSSTISS 292
>SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 774
Score = 26.6 bits (56), Expect = 5.2
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 250 KKSEVITNVVNKLIRNNKMNCMEYAYQLWLQ 342
K+ +T+ NKL+ ++ + +YAY L LQ
Sbjct: 35 KREAQLTDTPNKLLTDHDQSASDYAYALKLQ 65
>SPAC17G6.07c |||SNARE Slt1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 222
Score = 26.6 bits (56), Expect = 5.2
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = -1
Query: 592 LXXXVHNLEVGHXGXLTXRNELPADFWTRLVLAIAVGKSAIVAVLIVQ 449
L H+L + G + FW +L + IAV S IV + I+Q
Sbjct: 169 LDKNAHSLRHANHGVQSYSKSKRLSFWLQLGMIIAVVVSFIVMIFILQ 216
>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 910
Score = 26.6 bits (56), Expect = 5.2
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 5/61 (8%)
Frame = +1
Query: 109 FLCLFVASLYANETSVSDSKLEDDLY-----NSILVADYDHSVEKSKQIYEDKKSEVITN 273
F L +L N S K++D L+ N L +HS K K+ YE S ++TN
Sbjct: 731 FYYLCSINLMENVEEESIDKIKDILFDIHVDNLYLFPQLEHSFYKRKKNYEKALSVLVTN 790
Query: 274 V 276
+
Sbjct: 791 I 791
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 25.8 bits (54), Expect = 9.0
Identities = 13/33 (39%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +1
Query: 349 KDIVRECFPV*VQTYIRRKQH*A-DVQARRSRF 444
+DI+ E + V ++TY RRK+H +++ R RF
Sbjct: 1045 EDILTEFYEVRLRTYQRRKEHMVNELEKRFDRF 1077
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 9.0
Identities = 16/60 (26%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 193 ILVADYDHSVEKSKQIYEDKKSEVITNVVN-KLIRNNKMNCMEYAYQLWLQGSKDIVREC 369
+LV + + E+S + + + N+ KL NN +E Q +LQG K+ +++C
Sbjct: 152 VLVIELLETQERSSKRRRREGPTMKANIQRQKLDFNNLKKAIELRNQKFLQGIKEYIKKC 211
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,804,087
Number of Sequences: 5004
Number of extensions: 47592
Number of successful extensions: 192
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 489310570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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