Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_A21
(963 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P36188 Cluster: Troponin I; n=50; cellular organisms|Re... 124 3e-27
UniRef50_Q9XUN9 Cluster: Troponin I 3; n=6; Caenorhabditis|Rep: ... 80 7e-14
UniRef50_Q5DDC3 Cluster: SJCHGC00516 protein; n=4; Schistosoma j... 64 5e-09
UniRef50_Q7M3Y3 Cluster: Troponin I; n=5; Pectinidae|Rep: Tropon... 64 5e-09
UniRef50_UPI0000E48440 Cluster: PREDICTED: similar to troponin I... 62 3e-08
UniRef50_Q8WPC1 Cluster: Adult cardiac troponin I; n=9; Ascidiac... 55 2e-06
UniRef50_Q71N42 Cluster: Fast muscle troponin I; n=31; Euteleost... 50 1e-04
UniRef50_P50754 Cluster: Troponin I, cardiac muscle; n=18; Tetra... 48 3e-04
UniRef50_Q9NDR9 Cluster: Troponin I; n=1; Branchiostoma belcheri... 48 4e-04
UniRef50_P19429 Cluster: Troponin I, cardiac muscle; n=108; Chor... 46 0.001
UniRef50_P48788 Cluster: Troponin I, fast skeletal muscle; n=15;... 45 0.003
UniRef50_P24152 Cluster: Extensin precursor; n=9; Poaceae|Rep: E... 41 0.041
UniRef50_P45379 Cluster: Troponin T, cardiac muscle; n=294; Eute... 40 0.072
UniRef50_Q4RGJ7 Cluster: Chromosome undetermined SCAF15099, whol... 38 0.29
UniRef50_Q23D13 Cluster: Viral A-type inclusion protein repeat c... 37 0.67
UniRef50_P45378 Cluster: Troponin T, fast skeletal muscle; n=64;... 37 0.88
UniRef50_Q6AHQ1 Cluster: Troponin t protein 3, isoform b; n=9; R... 36 1.5
UniRef50_Q25148 Cluster: Troponin T; n=2; Halocynthia roretzi|Re... 35 3.6
UniRef50_Q9H6N6 Cluster: CDNA: FLJ22037 fis, clone HEP08868; n=2... 35 3.6
UniRef50_Q22M90 Cluster: Putative uncharacterized protein; n=1; ... 34 6.2
UniRef50_P19351 Cluster: Troponin T, skeletal muscle; n=46; Panc... 34 6.2
UniRef50_Q54QF2 Cluster: Putative uncharacterized protein; n=1; ... 33 8.2
>UniRef50_P36188 Cluster: Troponin I; n=50; cellular organisms|Rep:
Troponin I - Drosophila melanogaster (Fruit fly)
Length = 269
Score = 124 bits (300), Expect = 3e-27
Identities = 63/146 (43%), Positives = 83/146 (56%)
Frame = +2
Query: 179 IDRKRAEVRKRMEEASXXXXXXXGFMTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 358
I+RKRAEVRKRMEEAS GFMTP
Sbjct: 75 IERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRI 134
Query: 359 XXXXCGKPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVND 538
CG P+N+ DA+E ++ +C +Y+ER+ E +K+DLEY V++KD EI+ LN+QVND
Sbjct: 135 IEERCGSPRNLSDASEGELQEICEEYYERMYICEGQKWDLEYEVRKKDWEINDLNAQVND 194
Query: 539 XXGQFVKPTLKKVSPYENXFAXSRRR 616
G+FVKP LKKVS YEN FA +++
Sbjct: 195 LRGKFVKPALKKVSKYENKFAKLQKK 220
Score = 39.9 bits (89), Expect = 0.095
Identities = 20/36 (55%), Positives = 23/36 (63%)
Frame = +1
Query: 604 LQKKAXEFHXRNQLKVXKKXESPXEXXDXETQPXXS 711
LQKKA EF+ RNQLKV KK E E + E +P S
Sbjct: 217 LQKKAAEFNFRNQLKVVKKKEFTLEEEEKEKKPDWS 252
>UniRef50_Q9XUN9 Cluster: Troponin I 3; n=6; Caenorhabditis|Rep:
Troponin I 3 - Caenorhabditis elegans
Length = 260
Score = 80.2 bits (189), Expect = 7e-14
Identities = 51/159 (32%), Positives = 70/159 (44%), Gaps = 1/159 (0%)
Frame = +2
Query: 188 KRAEVRKRMEEASXXXXXXXGFMTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 367
K+AEVRKRMEEA+ GF+TP
Sbjct: 31 KKAEVRKRMEEAAKKGSKKKGFLTPERKKKLRKLLMMKAAEDLKQQQMLKEQERQKTLQQ 90
Query: 368 XCGKPKNIDDANED-TIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXX 544
++D N+ + + D R+ LE+EKFD+ + V + + EI+ L QVND
Sbjct: 91 RTIPLPDVDSINDQGQLLKIYEDMFARVCALEEEKFDINFGVSQTEAEINQLTIQVNDLR 150
Query: 545 GQFVKPTLKKVSPYENXFAXSRRRPXNSTXVTN*RSXKR 661
G+FVKPTLKKVS Y+N F S S N + K+
Sbjct: 151 GKFVKPTLKKVSKYDNKFKSSGEVKEKSNFRNNLKVVKK 189
>UniRef50_Q5DDC3 Cluster: SJCHGC00516 protein; n=4; Schistosoma
japonicum|Rep: SJCHGC00516 protein - Schistosoma
japonicum (Blood fluke)
Length = 221
Score = 64.1 bits (149), Expect = 5e-09
Identities = 30/80 (37%), Positives = 48/80 (60%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFV 556
+P +D E+ + +ER+ +LE +K+D E ++R+D EI+ L +VND G+FV
Sbjct: 108 EPLKLDGLGENDLNAKVKQLYERVRQLEGDKYDWEEKLRRQDFEINELTIKVNDVKGKFV 167
Query: 557 KPTLKKVSPYENXFAXSRRR 616
KP LKKVS E+ A ++
Sbjct: 168 KPVLKKVSKTESHMARFEKK 187
>UniRef50_Q7M3Y3 Cluster: Troponin I; n=5; Pectinidae|Rep: Troponin
I - Chlamys nipponensis akazara (Akazara scallop)
(Japanese scallop)
Length = 292
Score = 64.1 bits (149), Expect = 5e-09
Identities = 29/80 (36%), Positives = 52/80 (65%), Gaps = 1/80 (1%)
Frame = +2
Query: 380 PK-NIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFV 556
PK + D + ++ +C D+H+R+A LE++ +D E ++++D EI+ L +VND G+FV
Sbjct: 184 PKFSTDGKDVAALQALCKDFHKRLASLEEDVYDWEAKIRKQDFEINELTLKVNDTKGKFV 243
Query: 557 KPTLKKVSPYENXFAXSRRR 616
KP L+KV+ E+ +R+
Sbjct: 244 KPVLRKVNKTESKLDKIQRK 263
>UniRef50_UPI0000E48440 Cluster: PREDICTED: similar to troponin I,
partial; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to troponin I, partial -
Strongylocentrotus purpuratus
Length = 312
Score = 61.7 bits (143), Expect = 3e-08
Identities = 25/67 (37%), Positives = 43/67 (64%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFV 556
KP +ID + D ++ +C + E++ E KFDLE I++++D+EI + + N+ G+F
Sbjct: 230 KPLSIDSLSRDRLRALCEELTEQLTDAEGNKFDLEVIIRKQDLEIEDMKVRANEARGKFE 289
Query: 557 KPTLKKV 577
+P LKKV
Sbjct: 290 RPELKKV 296
>UniRef50_Q8WPC1 Cluster: Adult cardiac troponin I; n=9;
Ascidiacea|Rep: Adult cardiac troponin I - Halocynthia
roretzi (Sea squirt)
Length = 260
Score = 55.2 bits (127), Expect = 2e-06
Identities = 20/64 (31%), Positives = 41/64 (64%)
Frame = +2
Query: 386 NIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPT 565
N+ +E +K +C + H +I +++++++D+E V + D EI LN ++ D G+F +P
Sbjct: 132 NLGGMSEQDLKDLCRELHAKIEKVDEQRYDIEVKVNKNDQEIEDLNQRIFDLRGKFKRPP 191
Query: 566 LKKV 577
L++V
Sbjct: 192 LRRV 195
>UniRef50_Q71N42 Cluster: Fast muscle troponin I; n=31;
Euteleostomi|Rep: Fast muscle troponin I - Danio rerio
(Zebrafish) (Brachydanio rerio)
Length = 176
Score = 49.6 bits (113), Expect = 1e-04
Identities = 21/55 (38%), Positives = 36/55 (65%)
Frame = +2
Query: 413 IKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPTLKKV 577
++ +C H++I ++++E++D+E V + D EI L +V D G+F KP LKKV
Sbjct: 57 LQDLCKKLHQQIDKVDEERYDMEAKVAKADKEIEDLKVKVIDLKGKFKKPALKKV 111
>UniRef50_P50754 Cluster: Troponin I, cardiac muscle; n=18;
Tetrapoda|Rep: Troponin I, cardiac muscle - Xenopus
laevis (African clawed frog)
Length = 244
Score = 48.4 bits (110), Expect = 3e-04
Identities = 19/67 (28%), Positives = 39/67 (58%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFV 556
+P + + ++ +C + H RI +++E++D+E V + E+ LN ++ D G+F
Sbjct: 106 QPLQLSGLSRSELQDLCQELHARIDVVDEERYDMEAKVNKNITELEDLNQKIFDLRGKFK 165
Query: 557 KPTLKKV 577
KP L++V
Sbjct: 166 KPNLRRV 172
>UniRef50_Q9NDR9 Cluster: Troponin I; n=1; Branchiostoma
belcheri|Rep: Troponin I - Branchiostoma belcheri
(Amphioxus)
Length = 180
Score = 48.0 bits (109), Expect = 4e-04
Identities = 21/66 (31%), Positives = 37/66 (56%)
Frame = +2
Query: 380 PKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVK 559
P + D ++ +C H +I + E+EK+D+E V+ D E++ L ++ G+F K
Sbjct: 50 PISTSGLGHDELEELCRRLHAQITKAEEEKYDVEQKVEINDKELADLTQRMYSLTGKFKK 109
Query: 560 PTLKKV 577
P LK+V
Sbjct: 110 PQLKRV 115
>UniRef50_P19429 Cluster: Troponin I, cardiac muscle; n=108;
Chordata|Rep: Troponin I, cardiac muscle - Homo sapiens
(Human)
Length = 210
Score = 46.0 bits (104), Expect = 0.001
Identities = 17/55 (30%), Positives = 36/55 (65%)
Frame = +2
Query: 413 IKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPTLKKV 577
++ +C H R+ ++++E++D+E V + EI+ L ++ D G+F +PTL++V
Sbjct: 93 LQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRV 147
>UniRef50_P48788 Cluster: Troponin I, fast skeletal muscle; n=15;
Eutheria|Rep: Troponin I, fast skeletal muscle - Homo
sapiens (Human)
Length = 182
Score = 45.2 bits (102), Expect = 0.003
Identities = 17/55 (30%), Positives = 34/55 (61%)
Frame = +2
Query: 413 IKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPTLKKV 577
++ +C H +I E+EK+D+E V++ E+ +N ++ D G+F +P L++V
Sbjct: 61 VQELCKQLHAKIDAAEEEKYDMEVRVQKTSKELEDMNQKLFDLRGKFKRPPLRRV 115
>UniRef50_P24152 Cluster: Extensin precursor; n=9; Poaceae|Rep:
Extensin precursor - Sorghum bicolor (Sorghum) (Sorghum
vulgare)
Length = 283
Score = 41.1 bits (92), Expect = 0.041
Identities = 24/67 (35%), Positives = 27/67 (40%), Gaps = 2/67 (2%)
Frame = +1
Query: 769 TPXLXXTXXPTXXPLHXD-VPHTXVXSAHTHXTXYFXLXPPPS-SPXCYXPXISXLXLSP 942
TP T PT P H PHT AH PP+ +P Y P +S SP
Sbjct: 215 TPKPPATKPPTSTPTHPKPTPHTPYPQAHPPTYKPAPKPSPPAPTPPTYTPPVSHTPSSP 274
Query: 943 XPAPPXP 963
P PP P
Sbjct: 275 PPPPPPP 281
>UniRef50_P45379 Cluster: Troponin T, cardiac muscle; n=294;
Euteleostomi|Rep: Troponin T, cardiac muscle - Homo
sapiens (Human)
Length = 298
Score = 40.3 bits (90), Expect = 0.072
Identities = 19/50 (38%), Positives = 30/50 (60%)
Frame = +2
Query: 389 IDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVND 538
ID NED ++ + + I LE EKFDL+ K++ EI+ L +++ND
Sbjct: 231 IDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRIND 280
>UniRef50_Q4RGJ7 Cluster: Chromosome undetermined SCAF15099, whole
genome shotgun sequence; n=2; Tetraodontidae|Rep:
Chromosome undetermined SCAF15099, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 334
Score = 38.3 bits (85), Expect = 0.29
Identities = 19/72 (26%), Positives = 39/72 (54%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFV 556
KP N+D+ ++D ++ + E + LE+ KFD +KR+ E+ L +++++
Sbjct: 262 KPLNVDNLSDDKLRETAKELWELLYNLEEIKFDHCEKLKRQRYEVISLRNRIDELQKHLE 321
Query: 557 KPTLKKVSPYEN 592
K T+ K + +N
Sbjct: 322 KMTVGKKNKNKN 333
>UniRef50_Q23D13 Cluster: Viral A-type inclusion protein repeat
containing protein; n=1; Tetrahymena thermophila
SB210|Rep: Viral A-type inclusion protein repeat
containing protein - Tetrahymena thermophila SB210
Length = 1753
Score = 37.1 bits (82), Expect = 0.67
Identities = 21/56 (37%), Positives = 27/56 (48%)
Frame = +2
Query: 392 DDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVK 559
D A E+ + ERI LE KFDLE ++ K+ E L QVN+ Q K
Sbjct: 1105 DQAKEERSNSTLTEKEERIQNLEKSKFDLESSLQDKENECERLVQQVNNFQQQVKK 1160
>UniRef50_P45378 Cluster: Troponin T, fast skeletal muscle; n=64;
Euteleostomi|Rep: Troponin T, fast skeletal muscle -
Homo sapiens (Human)
Length = 269
Score = 36.7 bits (81), Expect = 0.88
Identities = 17/53 (32%), Positives = 31/53 (58%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVN 535
KP NID ED ++ + E + +LE +KF+ +KR+ +I+ L S+++
Sbjct: 195 KPLNIDHLGEDKLRDKAKELWETLHQLEIDKFEFGEKLKRQKYDITTLRSRID 247
>UniRef50_Q6AHQ1 Cluster: Troponin t protein 3, isoform b; n=9;
Rhabditida|Rep: Troponin t protein 3, isoform b -
Caenorhabditis elegans
Length = 1178
Score = 35.9 bits (79), Expect = 1.5
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +2
Query: 386 NIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQ 529
N + + +K + H+RI +LE EK+DLE +R++ ++ LN +
Sbjct: 1043 NASEVPQSELKAKIKELHQRICKLETEKYDLEKRHERQEYDMKELNER 1090
>UniRef50_Q25148 Cluster: Troponin T; n=2; Halocynthia roretzi|Rep:
Troponin T - Halocynthia roretzi (Sea squirt)
Length = 248
Score = 34.7 bits (76), Expect = 3.6
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +2
Query: 386 NIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVK 559
++D + D ++ + + + LE+EK D E + R+ +++ L +VN+ G++ K
Sbjct: 170 SVDHLSPDKLREKAQELWDLLYSLEEEKIDYEVRIDRQKYDLNQLRQRVNEYMGKYSK 227
>UniRef50_Q9H6N6 Cluster: CDNA: FLJ22037 fis, clone HEP08868; n=28;
Eutheria|Rep: CDNA: FLJ22037 fis, clone HEP08868 - Homo
sapiens (Human)
Length = 746
Score = 34.7 bits (76), Expect = 3.6
Identities = 16/45 (35%), Positives = 29/45 (64%)
Frame = +2
Query: 440 ERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPTLKK 574
+ + +E K DLE +VK++D+EI+ +NS+ D Q + TL++
Sbjct: 256 DNLNEMERSKLDLEEVVKKRDLEINSVNSKYEDE--QSLNSTLQR 298
>UniRef50_Q22M90 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 1698
Score = 33.9 bits (74), Expect = 6.2
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +2
Query: 401 NEDTIKX--VCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQVNDXXGQFVKPTLKK 574
NE+ +K + +Y + I L + DLE +++ ++I N+Q+N+ QF + LK
Sbjct: 193 NEEKVKINQMIDEYEQIIDELNQKNNDLEQRLQQSYIQIQNANNQLNEKQNQFAEEQLKW 252
Query: 575 VSPYEN 592
EN
Sbjct: 253 KKENEN 258
>UniRef50_P19351 Cluster: Troponin T, skeletal muscle; n=46;
Pancrustacea|Rep: Troponin T, skeletal muscle -
Drosophila melanogaster (Fruit fly)
Length = 397
Score = 33.9 bits (74), Expect = 6.2
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +2
Query: 377 KPKNIDDANEDTIKXVCXDYHERIARLEDEKFDLEYIVKRKDMEISXLNSQ 529
KP I+ E ++ + E I +LE EK+DLE KR+D ++ L +
Sbjct: 185 KPLAIEGFGEAKLREKAQELWELIVKLETEKYDLEERQKRQDYDLKELKER 235
>UniRef50_Q54QF2 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 495
Score = 33.5 bits (73), Expect = 8.2
Identities = 18/55 (32%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +2
Query: 458 EDEKFDLEYIVK--RKDMEISXLNSQVNDXXGQFVKPTLKKVSPYENXFAXSRRR 616
EDE+ ++ + K R++ +I L +Q N+ G++VKP K YE F S ++
Sbjct: 30 EDEQKKIDQLKKEMREEEQIRDLKNQFNEASGKYVKPDNKMDWMYEGAFQLSMKK 84
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 619,335,461
Number of Sequences: 1657284
Number of extensions: 9073402
Number of successful extensions: 21918
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 19218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21782
length of database: 575,637,011
effective HSP length: 101
effective length of database: 408,251,327
effective search space used: 89407040613
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -