Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP01_F_A18
(961 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 66 5e-12
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 37 0.004
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 36 0.008
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 31 0.32
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.7
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 5.2
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 6.9
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 6.9
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 9.1
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 9.1
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 66.5 bits (155), Expect = 5e-12
Identities = 35/90 (38%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
Frame = +1
Query: 277 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 453
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 454 MHGETPYEIMFGPDICGPGTKKVHVIFSYK 543
M Y IMFGPD CG +VH IF +K
Sbjct: 148 MSNSIDYRIMFGPDKCGV-NDRVHFIFKHK 176
Score = 37.9 bits (84), Expect = 0.002
Identities = 20/54 (37%), Positives = 23/54 (42%), Gaps = 2/54 (3%)
Frame = +2
Query: 710 LPPXKIXDP*SPXTXXTXDDXPLFXTPXTXXPEDWDK--PEXXPXPXAPXPEXW 865
LPP +I DP D P P P+DWD+ P P P A PE W
Sbjct: 237 LPPVEIYDP-EDIKPADWVDEPEIPDPNAVKPDDWDEDAPRMIPDPDAVKPEDW 289
Score = 35.9 bits (79), Expect = 0.008
Identities = 19/52 (36%), Positives = 24/52 (46%), Gaps = 3/52 (5%)
Frame = +2
Query: 722 KIXDP*SPXTXXTXDDXP-LFXTPXTXXPEDW--DKPEXXPXPXAPXPEXWD 868
+I DP + +D P + P PEDW D+P P P A PE WD
Sbjct: 258 EIPDPNAVKPDDWDEDAPRMIPDPDAVKPEDWLEDEPLYIPDPEAQKPEDWD 309
Score = 29.5 bits (63), Expect = 0.74
Identities = 15/43 (34%), Positives = 18/43 (41%), Gaps = 1/43 (2%)
Frame = +2
Query: 788 PXTXXPEDW-DKPEXXPXPXAPXPEXWDXXNXXXXGTPPXIXP 913
P P DW D+PE P P A P+ WD P + P
Sbjct: 245 PEDIKPADWVDEPEI-PDPNAVKPDDWDEDAPRMIPDPDAVKP 286
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/38 (36%), Positives = 17/38 (44%), Gaps = 1/38 (2%)
Frame = +2
Query: 716 PXKIXDP*SPXTXXTXDDXPLFXT-PXTXXPEDWDKPE 826
P I DP + +D PL+ P PEDWD E
Sbjct: 275 PRMIPDPDAVKPEDWLEDEPLYIPDPEAQKPEDWDDEE 312
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 37.1 bits (82), Expect = 0.004
Identities = 38/137 (27%), Positives = 57/137 (41%), Gaps = 6/137 (4%)
Frame = +1
Query: 157 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 324
F E+F + +W S W K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 325 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 498
P + + P+V+ F VK + CG Y+ + + K++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 499 CGPGTKKVHVIFSYKGK 549
CG I SY GK
Sbjct: 149 CGMFDYISLSIISYDGK 165
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 35.9 bits (79), Expect = 0.008
Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
Frame = -3
Query: 311 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQFIE 135
N SSE F S+S SL P VN + S + ++ L S + S+ NF+S ++S +
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531
Query: 134 DNASKLTTTSTT 99
+ S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 30.7 bits (66), Expect = 0.32
Identities = 23/71 (32%), Positives = 28/71 (39%), Gaps = 4/71 (5%)
Frame = -3
Query: 242 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT----TSTTAFIFDSQY 75
N N F + + S +SGNFS T Q DN S +T ST F S
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFSTQPGRLSTGLHHFPSDR 634
Query: 74 HLLQQKTGHES 42
LL +T S
Sbjct: 635 DLLSNQTRRSS 645
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 28.3 bits (60), Expect = 1.7
Identities = 32/142 (22%), Positives = 57/142 (40%), Gaps = 3/142 (2%)
Frame = -3
Query: 443 SSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGS 264
+SL S P S S + T ++ +PS + + A ++SS PLSSS
Sbjct: 12 TSLSSSASSSIPASSSSAAASTSLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSF 71
Query: 263 LKNFPA---VNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTTTSTTAF 93
+ +L +S S L S S+ SS + S ++ L+++S++
Sbjct: 72 TSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSSSSSHALSSSSSSLV 131
Query: 92 IFDSQYHLLQQKTGHESNLKNS 27
S + H S++ +S
Sbjct: 132 ASSSSSGMSSSSLSHSSSVPSS 153
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 5.2
Identities = 33/137 (24%), Positives = 59/137 (43%)
Frame = -3
Query: 503 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 324
P S I S SP S SS S + S S + + + T+ + + + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392
Query: 323 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQ 144
+ + SS + SSS S + V+ F ++ S ++ SH SS + SS ++
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHN----STSSRSSSHSSSHSLSSLSSKP 448
Query: 143 FIEDNASKLTTTSTTAF 93
+ ++S L T+S+ +
Sbjct: 449 ILTASSSSLLTSSSHTY 465
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.9
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -3
Query: 311 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 216
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 163 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 306
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 9.1
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = -3
Query: 335 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 174
++LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 9.1
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 160 EEKFPDDSWESNWVYS 207
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,347,418
Number of Sequences: 5004
Number of extensions: 64350
Number of successful extensions: 206
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 491307756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -