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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP01_F_A18
         (961 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             134   1e-31
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                44   1e-04
SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   0.003
SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.4  
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   7.4  
SB_19075| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.8  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  134 bits (324), Expect = 1e-31
 Identities = 61/92 (66%), Positives = 71/92 (77%), Gaps = 1/92 (1%)
 Frame = +1

Query: 151  VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 330
            V F EKF D SWE  WV S   G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756  VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815

Query: 331  F-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLK 423
            F KPF+NEGK LV+QF+VKHEQ+IDCGGGY K
Sbjct: 816  FEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK 847



 Score = 57.6 bits (133), Expect = 1e-08
 Identities = 31/78 (39%), Positives = 33/78 (42%)
 Frame = +2

Query: 710  LPPXKIXDP*SPXTXXTXDDXPLFXTPXTXXPEDWDKPEXXPXPXAPXPEXWDXXNXXXX 889
            LPP  I DP         DD  +   P    PEDWDKP+  P P A  PE WD       
Sbjct: 886  LPPKTIKDP-EAKKPEDWDDKKMIDDPEDKKPEDWDKPQNIPDPDAKKPEDWD-DEIDGE 943

Query: 890  GTPPXIXPPXXKGLXGPK 943
              PP I  P  KG   PK
Sbjct: 944  WEPPMIDNPEYKGEWKPK 961


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 44.4 bits (100), Expect = 1e-04
 Identities = 22/48 (45%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
 Frame = +1

Query: 433 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNXLXSKKY 573
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN    +K+
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGE-DRKLHFIFRHKNPKNGTIEEKH 74



 Score = 35.9 bits (79), Expect = 0.049
 Identities = 13/27 (48%), Positives = 15/27 (55%)
 Frame = +2

Query: 788 PXTXXPEDWDKPEXXPXPXAPXPEXWD 868
           P    PEDWD+ E  P P A  P+ WD
Sbjct: 135 PDDKRPEDWDEREKIPDPDAEKPDDWD 161



 Score = 33.9 bits (74), Expect = 0.20
 Identities = 23/72 (31%), Positives = 27/72 (37%), Gaps = 4/72 (5%)
 Frame = +2

Query: 722 KIXDP*SPXTXXTXDDXPLFXTPXTXX--PEDW--DKPEXXPXPXAPXPEXWDXXNXXXX 889
           KI DP +       +D P+   P T    PE W  D+PE  P P    PE WD       
Sbjct: 148 KIPDPDAEKPDDWDEDAPV-KIPDTNAKKPEGWLDDEPEYVPDPNGIKPEDWDDEEDGEW 206

Query: 890 GTPPXIXPPXXK 925
             P    P   K
Sbjct: 207 EAPQIANPKCEK 218


>SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 539

 Score = 39.9 bits (89), Expect = 0.003
 Identities = 30/98 (30%), Positives = 44/98 (44%), Gaps = 3/98 (3%)
 Frame = -3

Query: 410 PPQSMSCSCLTVNWTTKGLPSLLNG---LNLRERA*NLASSEVFKPLSSSGSLKNFPAVN 240
           P +  SC CL V W T GLP L++G   L    R   L+   +  P   S SL +   V 
Sbjct: 429 PSEQNSCQCLFVGWQTVGLPQLIHGTVALISFRRKRTLSGGSL--PRGRSRSLSS-DRVG 485

Query: 239 LNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNA 126
              P++  G +    +    S+G+  S+ +SQ   D A
Sbjct: 486 TRLPSARKGSASKIGIPRTSSTGSLGSRRSSQSSTDGA 523


>SB_50300| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3669

 Score = 31.1 bits (67), Expect = 1.4
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -3

Query: 371  WTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGSLKNF 252
            +TT+GLPS ++ +NL E   NL S    KP   S  + ++
Sbjct: 959  YTTQGLPSKISSVNLTEALSNLISISWSKPSDGSSLITDY 998


>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 125

 Score = 28.7 bits (61), Expect = 7.4
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = -2

Query: 213 VLTVHPIAFPRIIRKLLLKEYI 148
           +LT H +  P++IRK+ LK+Y+
Sbjct: 73  LLTAHLVVAPKLIRKMPLKDYV 94


>SB_19075| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 6500

 Score = 28.3 bits (60), Expect = 9.8
 Identities = 20/84 (23%), Positives = 33/84 (39%), Gaps = 2/84 (2%)
 Frame = -3

Query: 251  PAVNLNFPNSFPGCSLYTQLLSH--ESSGNFSSKNTSQFIEDNASKLTTTSTTAFIFDSQ 78
            P+V LNF N+FPG  L  + +S   + SG  +     + +  N   +    +  FI+   
Sbjct: 6188 PSVELNFRNTFPGDDLLKRYISDILQDSGGSALDLALEGLNVNIVIVIKAESDPFIYQGA 6247

Query: 77   YHLLQQKTGHESNLKNSLX*VVXK 6
              +     G     K  L  +  K
Sbjct: 6248 RKVFDPAQGARPKAKKVLVIITDK 6271


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,547,473
Number of Sequences: 59808
Number of extensions: 507897
Number of successful extensions: 1141
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1050
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1134
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2824376637
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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