BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP01_F_A10 (971 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.11 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 0.99 SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 29 1.3 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 3.0 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 5.3 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 7.0 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 7.0 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 32.3 bits (70), Expect = 0.11 Identities = 28/82 (34%), Positives = 29/82 (35%), Gaps = 9/82 (10%) Frame = -1 Query: 908 GGXWGKXGXFXGGNPP---------EFXGEXXXXRXXGGGGNRXAPXRXGPXGXGXSXXG 756 GG +G G GG PP F GE GG G GP G G G Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFG-GGPG 245 Query: 755 GKGEGXAQKRXXGXXXGGVGGG 690 G G G G GG GGG Sbjct: 246 GFGGGLG---GFGGGPGGFGGG 264 Score = 26.2 bits (55), Expect = 7.0 Identities = 24/90 (26%), Positives = 26/90 (28%), Gaps = 1/90 (1%) Frame = -1 Query: 896 GKXGXFXGGNPPEFXGEXXXXRXXGGGGNRXAPXRXGPXGXGXSXXGGKGEGXAQKRXXG 717 G G GG G R GGG G G GG G + G Sbjct: 162 GLGGLALGGLASHALGNLFHHRGHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHG 221 Query: 716 XXXG-GVGGGXXXGXXRXXPXTPXGXGGXI 630 G G G G G P G GG + Sbjct: 222 GHGGFGGGPGGFEGGPGGFGGGPGGFGGGL 251 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 29.1 bits (62), Expect = 0.99 Identities = 12/33 (36%), Positives = 14/33 (42%) Frame = +1 Query: 814 PPPPXXRXXXXSPXNSGGLPPXXXPFXPHXPPP 912 PPP SP ++ LP P P PPP Sbjct: 172 PPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPP 204 Score = 28.7 bits (61), Expect = 1.3 Identities = 13/42 (30%), Positives = 16/42 (38%) Frame = +1 Query: 820 PPXXRXXXXSPXNSGGLPPXXXPFXPHXPPPNXPXNPXKXXP 945 PP SP ++ +P P PPP P P K P Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185 Score = 26.6 bits (56), Expect = 5.3 Identities = 16/53 (30%), Positives = 17/53 (32%), Gaps = 2/53 (3%) Frame = +1 Query: 814 PPPPXXRXXXXS--PXNSGGLPPXXXPFXPHXPPPNXPXNPXKXXPFXXPXXP 966 PP P S P LPP P P PP+ P P P P Sbjct: 151 PPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPP 203 >SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 194 Score = 28.7 bits (61), Expect = 1.3 Identities = 16/40 (40%), Positives = 18/40 (45%), Gaps = 1/40 (2%) Frame = -1 Query: 965 GXXGXXKGXXFXGLXGXFGGGX-WGKXGXFXGGNPPEFXG 849 G G +G G G FGGG G G F GG+ F G Sbjct: 153 GFGGNSRGGFGGGSRGGFGGGSRGGSRGGFRGGSRGGFRG 192 Score = 27.9 bits (59), Expect = 2.3 Identities = 15/47 (31%), Positives = 18/47 (38%) Frame = -1 Query: 818 GGNRXAPXRXGPXGXGXSXXGGKGEGXAQKRXXGXXXGGVGGGXXXG 678 G + R GP G G G G ++ G GG GGG G Sbjct: 123 GPKKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGG 169 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 27.5 bits (58), Expect = 3.0 Identities = 20/55 (36%), Positives = 21/55 (38%), Gaps = 1/55 (1%) Frame = -1 Query: 908 GGXWGKXGXFXGGNPPEFXGEXXXXRXXGGGGNRXA-PXRXGPXGXGXSXXGGKG 747 GG G G F GG F G R G GG R R G G GG+G Sbjct: 12 GGSRGGRGGFNGGR-GGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRG 65 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.6 bits (56), Expect = 5.3 Identities = 14/40 (35%), Positives = 17/40 (42%), Gaps = 1/40 (2%) Frame = +1 Query: 814 PPPPXXRXXXXSPXNSGG-LPPXXXPFXPHXPPPNXPXNP 930 PPPP P SG +PP P P+ P P +P Sbjct: 926 PPPPKVGETYHPPTASGTRVPPVQQPSHPNPYTPVAPQSP 965 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 26.2 bits (55), Expect = 7.0 Identities = 25/113 (22%), Positives = 30/113 (26%), Gaps = 3/113 (2%) Frame = +1 Query: 637 PPLPXGVXGXXLXXP---HXXPPPTPPXXXPXXLFWAXXXXXXXXXDXPXPXGPXRLGAX 807 PP P + P PPP PP P P P P + Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNP-PAPPPAIPGR 398 Query: 808 RFPPPPXXRXXXXSPXNSGGLPPXXXPFXPHXPPPNXPXNPXKXXPFXXPXXP 966 P P + PP P P PP+ P + P P P Sbjct: 399 SAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPP 451 Score = 25.8 bits (54), Expect = 9.2 Identities = 14/53 (26%), Positives = 15/53 (28%) Frame = +2 Query: 695 PPPPXXKXRXXXXXXXXXXXXXQXTXPXPXXPXXXXXPGFPPPPXXGXXGXPP 853 PPPP R + P P P G P P G PP Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPP 363 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 26.2 bits (55), Expect = 7.0 Identities = 14/53 (26%), Positives = 26/53 (49%) Frame = -2 Query: 259 VNAMIFIHNFHWINAGGDFAIGTVPAVFLRVDSYNRKHSDQCKSDRKFHDFYS 101 V+ ++ I NFHW + I V + ++D Y R + + +S + H Y+ Sbjct: 388 VDVLLAIMNFHWDESNELTPIVAVDNMLQKLDKYERLYPSR-RSILQEHSLYA 439 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,705,055 Number of Sequences: 5004 Number of extensions: 42891 Number of successful extensions: 128 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 117 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 499296500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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