Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0008_G20
(421 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 77 1e-15
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 55 4e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 48 6e-07
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 42 3e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 33 0.014
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 33 0.024
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.032
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 29 0.22
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 29 0.22
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 0.68
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|... 25 3.6
SPAPYUG7.06 |mug67||PPPDE peptidase family |Schizosaccharomyces ... 25 3.6
SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein Rga5|Sch... 25 4.8
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 25 4.8
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 4.8
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 25 6.3
SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 comple... 25 6.3
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 6.3
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 25 6.3
SPAC6B12.11 |drc1|sld1|DNA replication protein Drc1|Schizosaccha... 24 8.4
SPAP27G11.09c |||GTP cyclohydrolase |Schizosaccharomyces pombe|c... 24 8.4
SPBP8B7.08c |||leucine carboxyl methyltransferase |Schizosacchar... 24 8.4
SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit... 24 8.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 76.6 bits (180), Expect = 1e-15
Identities = 35/106 (33%), Positives = 67/106 (63%)
Frame = +1
Query: 52 DQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 231
D+++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F
Sbjct: 8 DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67
Query: 232 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 369
F + + +++ D E +E++EAF+++D++GNGYIT L +
Sbjct: 68 TEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHV 110
Score = 50.8 bits (116), Expect = 8e-08
Identities = 23/72 (31%), Positives = 45/72 (62%)
Frame = +1
Query: 40 DTDDDQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSG 219
DTD+++++ R+AF++FD +GYI V +++ +L ++G+ E+ +I E D + G
Sbjct: 80 DTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136
Query: 220 KINFDGFCNIAS 255
IN++ F + S
Sbjct: 137 VINYEEFSRVIS 148
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.2 bits (127), Expect = 4e-09
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = +1
Query: 76 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGF 240
KAF++FD SGYI+ K + + T+G+ D+E+Q ++ E DP NSG ++ F
Sbjct: 82 KAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
Score = 47.6 bits (108), Expect = 8e-07
Identities = 28/95 (29%), Positives = 53/95 (55%)
Frame = +1
Query: 70 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 249
+++AF ++D K G I + ++L ++G D+EL L +E I+ F +
Sbjct: 11 MKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEKKFMSF 66
Query: 250 ASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTS 354
S+ L E ++E +E +AFR++D++ +GYI T+
Sbjct: 67 VSNKLRETESE---EEYIKAFRVFDKDNSGYIETA 98
Score = 26.6 bits (56), Expect = 1.6
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 295 ELKEAFRLYDREGNGYITTS 354
E+KEAF LYD + +G I TS
Sbjct: 10 EMKEAFVLYDIDKDGLIPTS 29
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 48.0 bits (109), Expect = 6e-07
Identities = 24/100 (24%), Positives = 50/100 (50%)
Frame = +1
Query: 70 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 249
+ +AF++FD+ K ID ++ + +G + SE+ ++ + D G + + F +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 250 ASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 369
+ + E D +E+K AF L+D + G I+ L+ +
Sbjct: 99 MTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 45.2 bits (102), Expect = 4e-06
Identities = 19/60 (31%), Positives = 36/60 (60%)
Frame = +1
Query: 70 LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 249
+++AF++FD ++G I + + + + + DD EL+A+I+E D + G+IN F I
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171
Score = 24.6 bits (51), Expect = 6.3
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +1
Query: 262 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLK 363
L E E +Q++ EAF+L+D + + I L+
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELR 60
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 42.3 bits (95), Expect = 3e-05
Identities = 30/103 (29%), Positives = 53/103 (51%)
Frame = +1
Query: 52 DQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 231
++++ +RK F D +SG ID + +I + S L +++DE + G ++F
Sbjct: 20 NEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDE---DGGGDVDF 75
Query: 232 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTL 360
F N S F + E ++LK AF++YD + +GYI+ L
Sbjct: 76 QEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGEL 115
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 33.5 bits (73), Expect = 0.014
Identities = 23/103 (22%), Positives = 44/103 (42%), Gaps = 8/103 (7%)
Frame = +1
Query: 64 AMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFC 243
A F +FD K+GYID + L+ + + +L D +N+G I++D
Sbjct: 63 AFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEML 122
Query: 244 NIASHFLE--------EEDAEAMQQELKEAFRLYDREGNGYIT 348
I + ED + ++ + + F + D+ +G +T
Sbjct: 123 RIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLT 165
Score = 25.4 bits (53), Expect = 3.6
Identities = 26/108 (24%), Positives = 45/108 (41%), Gaps = 1/108 (0%)
Frame = +1
Query: 49 DDQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSEL-QALIDENDPENSGKI 225
D +++ K F F SG+++ + I D S + + + D + +G I
Sbjct: 23 DKKELQQWYKGF--FKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYI 80
Query: 226 NFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 369
+F F S E + +L AF+LYD + NG I+ + I
Sbjct: 81 DFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRI 124
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 32.7 bits (71), Expect = 0.024
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +1
Query: 76 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIAS 255
+AF +FD T GYID + +G+ +LQ ++D +GK++ + F +
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73
Query: 256 H 258
H
Sbjct: 74 H 74
Score = 24.6 bits (51), Expect = 6.3
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +1
Query: 286 MQQELKEAFRLYDREGNGYITTSTLK 363
M +E +EAF L+D GYI L+
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLR 35
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.032
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 268 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 366
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.5 bits (63), Expect = 0.22
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +1
Query: 124 LKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 261
+ +ST+ +T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 29.5 bits (63), Expect = 0.22
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1
Query: 292 QELKEAFRLYDREGNGYITTSTLK 363
QELKEAF L D++G+G I +K
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVK 71
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 0.68
Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +1
Query: 118 DVLKISTILNTM-GQLFDDSELQALIDENDP--ENSGKINFDGFCNIASHFLEEEDAEAM 288
+ ++I+ L + G+ D ++ + + +P ENS N D +A+ FL+E + E
Sbjct: 402 ETIRIAVNLQELIGKYLGDKDIFQIENFINPIEENSADTNDDIVNQVAAQFLDEREFETD 461
Query: 289 QQELKEAFRLYDREGNGYITT 351
++E + + L ++ I T
Sbjct: 462 EEEEESQYLLSTKDAINAINT 482
>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
Length = 624
Score = 25.4 bits (53), Expect = 3.6
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +2
Query: 146 TPWDNCSMIPNCRRSSMKMIQRTLEKLTSMVSAISLPISLK-KKTQKLC 289
TP S+ PN RRSS+++ + T +++I P L ++ Q+ C
Sbjct: 61 TPTMRPSLAPNTRRSSVRVSNASFISQTPNITSIKDPRPLSDRRYQQEC 109
>SPAPYUG7.06 |mug67||PPPDE peptidase family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 201
Score = 25.4 bits (53), Expect = 3.6
Identities = 25/94 (26%), Positives = 38/94 (40%), Gaps = 3/94 (3%)
Frame = +2
Query: 44 PMTIKRWRCSAKHSRCSTQRSPDIS-MF*RFQQS*TPWDNCSMIPNCRR-SSMKMIQRTL 217
P+ RWRCS C T PD+ + R Q T + NC ++ I+ T
Sbjct: 60 PLEGCRWRCSIALPNC-TLPKPDVDRILIRLSQEFTGLSYSLLERNCNHFTNAAAIELTG 118
Query: 218 EKLTSMVSAIS-LPISLKKKTQKLCNKN*KKLSD 316
+ S ++ IS + ++ T L K SD
Sbjct: 119 SPIPSFLNRISRIGLAFPTITNALLQHGQKNTSD 152
>SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein
Rga5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 361
Score = 25.0 bits (52), Expect = 4.8
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 50 TIKRWRCSAKHSRCSTQRSP 109
+IKRWR ++HS + SP
Sbjct: 295 SIKRWRSFSRHSSATYSNSP 314
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 25.0 bits (52), Expect = 4.8
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +1
Query: 301 KEAFRLYDREGNGYITTSTLKEI 369
K+AF L+DR G G I +++ ++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDL 31
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.0 bits (52), Expect = 4.8
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +1
Query: 226 NFDGFCNIASHFLEEEDAEAM 288
+ DGF ++ASHF AE++
Sbjct: 269 SIDGFVSVASHFSSASTAESL 289
>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 24.6 bits (51), Expect = 6.3
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +1
Query: 196 ENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFR 315
E D EN ++ A LE+ED EAM +L+E R
Sbjct: 152 EIDKENLAVLSQKSVLKTAEE-LEKEDREAMSAKLQELIR 190
>SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 442
Score = 24.6 bits (51), Expect = 6.3
Identities = 20/79 (25%), Positives = 34/79 (43%)
Frame = +1
Query: 4 LVTLRLIFTNNMDTDDDQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQ 183
LVT + N + DQ+ L + TT +ID + TI+ + +L+ LQ
Sbjct: 69 LVTTYFEYVENAASSPDQRKWELLQNLYSNLTTCFSHIDGAWLCTIVKRVSKLYVKLSLQ 128
Query: 184 ALIDENDPENSGKINFDGF 240
+D + P + +GF
Sbjct: 129 --LDTSTPTLEDSFDGNGF 145
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 24.6 bits (51), Expect = 6.3
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +1
Query: 193 DENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDR 327
D N+PE+ GF +I S +E D E+ + +YDR
Sbjct: 645 DCNNPESFSLFVDAGFISITSQNVETNDKESNSESETLYHLIYDR 689
>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 504
Score = 24.6 bits (51), Expect = 6.3
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +1
Query: 97 TTKSGYIDVLKISTILNTMGQLFDDSE 177
TTKS +I++ ST + ++F+DS+
Sbjct: 143 TTKSRFIEISATSTTVADCRKIFEDSQ 169
>SPAC6B12.11 |drc1|sld1|DNA replication protein
Drc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 337
Score = 24.2 bits (50), Expect = 8.4
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -1
Query: 139 LLKSSEHRYIRTSLCRTS 86
LLK EH Y+ T+ C+ S
Sbjct: 16 LLKKWEHEYVHTNNCKPS 33
>SPAP27G11.09c |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 326
Score = 24.2 bits (50), Expect = 8.4
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 81 IPDVRHNEVRIYRCSEDFNNPEH 149
IP V+ E+ ++ DF+N EH
Sbjct: 74 IPTVKGTEIFLHLYKNDFDNKEH 96
>SPBP8B7.08c |||leucine carboxyl methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 310
Score = 24.2 bits (50), Expect = 8.4
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = +1
Query: 169 DSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQEL 300
D+ + I E PEN I FD + N E EA++Q +
Sbjct: 79 DTRVFRYISEYGPENLKFIEFDFYPNCIRKIRTIEKHEALKQNI 122
>SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit
Rpb4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 135
Score = 24.2 bits (50), Expect = 8.4
Identities = 12/47 (25%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
Frame = +1
Query: 67 MLRKAFQMFDTTKSGYI---DVLKISTILNTMGQLFDDSELQALIDE 198
+L F F+ + G + D + T++ ++ DD LQ ++DE
Sbjct: 80 ILGNRFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDE 126
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,728,156
Number of Sequences: 5004
Number of extensions: 34203
Number of successful extensions: 138
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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