Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0008_G13
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc... 27 1.4
SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like protein|Schi... 27 2.5
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po... 26 3.3
SPAC9.02c |||N-acetyltransferase|Schizosaccharomyces pombe|chr 1... 26 4.3
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 26 4.3
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 5.7
SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr... 25 5.7
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 25 7.5
SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces ... 25 7.5
SPAC644.12 |cdc5||cell division control protein Cdc5|Schizosacch... 25 7.5
SPBC31F10.09c |nut2|med10|mediator complex subunit Med10|Schizos... 25 10.0
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 25 10.0
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 25 10.0
SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces ... 25 10.0
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 10.0
>SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 27.5 bits (58), Expect = 1.4
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2
Query: 353 VSLKFVHTLRLESVSYGHSLMQLQIAS 273
VS +F H LRL+ V+ H + +IAS
Sbjct: 291 VSTRFSHELRLKPVAVAHGIKNSEIAS 317
>SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 308
Score = 26.6 bits (56), Expect = 2.5
Identities = 13/38 (34%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
Frame = -3
Query: 181 VTHSGSRRHELSQP-PGIVSRPGSPPCRSRGAPTRRCP 71
++ S S H SQ PG S P P + G ++CP
Sbjct: 66 ISSSSSEPHHTSQSTPGETSSPVCPVSGAHGGADKKCP 103
>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 942
Score = 26.2 bits (55), Expect = 3.3
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +3
Query: 405 LGAVPRGAVPPRADRVLRDLQG 470
LG V RG +P DR+L LQG
Sbjct: 476 LGHVQRGGIPCAYDRMLATLQG 497
>SPAC9.02c |||N-acetyltransferase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 165
Score = 25.8 bits (54), Expect = 4.3
Identities = 18/70 (25%), Positives = 27/70 (38%)
Frame = +1
Query: 73 DISESEHLENGMAESLDERRFQEAERARVSEILNESRDAPDNEINLDDPCMKVHCSAGRV 252
+I E E L+ F EAERA + I + + AP+ ++ L P G V
Sbjct: 3 NIRNIERSEVEEVSKLEAICFPEAERASFARIKDRVKRAPEIQLGLFAPTQHTSTLIGHV 62
Query: 253 CEINEHGEAI 282
E +
Sbjct: 63 LTSRTGSETV 72
>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 25.8 bits (54), Expect = 4.3
Identities = 30/148 (20%), Positives = 53/148 (35%), Gaps = 1/148 (0%)
Frame = +1
Query: 52 SAGLPRGDISESEHLEN-GMAESLDERRFQEAERARVSEILNESRDAPDNEINLDDPCMK 228
S G IS E +N G+ E D E++ + E + P LDD +
Sbjct: 12 SNGFSEEYISHPERNDNQGIVEITDLVFSSESKLTQRKESRDSKTFVPSFLEELDDDHLH 71
Query: 229 VHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNETWSSDCEVYRQRCLCLDHS 408
+ G + +N GE + +++C + + + + + +Q L +
Sbjct: 72 ELVTNGGILYMNSLGEGVSGSVRKC--RIRGTQMIFAMKTVLAAPNTALQKQLLRELKIN 129
Query: 409 ELCRGAQYHHVQIEYYGTCREMPECSEN 492
C ++YYG C EC N
Sbjct: 130 RSCTSPYI----VKYYGACYNNAECQLN 153
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.4 bits (53), Expect = 5.7
Identities = 7/23 (30%), Positives = 13/23 (56%)
Frame = +1
Query: 235 CSAGRVCEINEHGEAICNCIKEC 303
C+A R+C++ E C+ +C
Sbjct: 974 CTASRICKVREIASLSLTCLLDC 996
>SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 222
Score = 25.4 bits (53), Expect = 5.7
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +1
Query: 205 NLDDPCMKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 351
NL K++ G + + HG I CIK+ + + K T FNE
Sbjct: 105 NLHKGAAKIYSMGGVIAAVC-HGPVILPCIKDSTGFSIVKGKTVTAFNE 152
>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 601
Score = 25.0 bits (52), Expect = 7.5
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = -2
Query: 218 GSSRLISLSGASRDSFRISETRALSAS 138
G+ +L +SRD+FR ++T+A S+S
Sbjct: 47 GNKKLKESQFSSRDNFRTTQTQASSSS 73
>SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 528
Score = 25.0 bits (52), Expect = 7.5
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +2
Query: 236 AAPAVSVRSTSTGKLSATASRSVHTKPTLGARCAQTSTRPG 358
++P+ + STST S+ S + T+PT G Q + G
Sbjct: 464 SSPSGTNSSTSTSSTSSKHSTGISTEPTTGEENKQDGSVGG 504
>SPAC644.12 |cdc5||cell division control protein
Cdc5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 757
Score = 25.0 bits (52), Expect = 7.5
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -3
Query: 436 GGTAPRGTAPSGRGRGSAVGTPRSPMTRS 350
GGT G PS GSA+ P++ R+
Sbjct: 409 GGTGYTGVTPSHAANGSALAAPQATPFRT 437
>SPBC31F10.09c |nut2|med10|mediator complex subunit
Med10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 138
Score = 24.6 bits (51), Expect = 10.0
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +3
Query: 417 PRGAVPPRADRVLRDLQGNAGVQRERNVRLP 509
P A+P D ++RDL+ + R+ N +P
Sbjct: 34 PSDAIPESLDTLIRDLKSLPDISRKVNNLIP 64
>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 24.6 bits (51), Expect = 10.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 354 GLVEVCAHLAPRVGFVWTLLDAVADS 277
G+V A +AP V +W +L+ + DS
Sbjct: 104 GMVLQWASIAPAVDAIWHILNVIDDS 129
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 24.6 bits (51), Expect = 10.0
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +1
Query: 109 AESLDERRFQEAERARVSEILNESRDAPDNEIN 207
A+ LDER F+ E S+ L ++DA N IN
Sbjct: 450 AQFLDEREFETDEEEEESQYLLSTKDA-INAIN 481
>SPAC17A2.03c |vma6||V-type ATPase subunit d|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 343
Score = 24.6 bits (51), Expect = 10.0
Identities = 19/74 (25%), Positives = 31/74 (41%), Gaps = 10/74 (13%)
Frame = +1
Query: 307 YETDSRRKVCTNFNETWS-----SDCEVYRQRCLCLD-----HSELCRGAQYHHVQIEYY 456
+ET V TN E +S + Y + CL D H E+ R Y ++Y
Sbjct: 131 FETLPALCVATNVEELYSVVLIETPLAPYFKDCLSADDLDEQHIEIIRNTLYKAYLEDFY 190
Query: 457 GTCREMPECSENEM 498
C+++ C+ + M
Sbjct: 191 NFCKKIGACTADTM 204
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 24.6 bits (51), Expect = 10.0
Identities = 16/56 (28%), Positives = 21/56 (37%)
Frame = -3
Query: 529 ASRACAAGSLTFRSRCTPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSAVGTPRSP 362
AS A S + S P S + S+RG T T P RG + +P
Sbjct: 264 ASSTIAEVSPMYGSHAAPYASTPSAAVGSSRGSTPASATVPISPARGFPTTSAYNP 319
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,097
Number of Sequences: 5004
Number of extensions: 43177
Number of successful extensions: 151
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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