BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0008_D07
(547 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.45
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.59
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 28 0.78
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 27 1.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 3.2
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 4.2
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 25 5.5
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 7.3
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3... 25 9.6
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 25 9.6
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 29.1 bits (62), Expect = 0.45
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -1
Query: 346 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 233
CI GF ++ + +RTR + PS +LS T V+ FL
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS-AFKTLSLTSVMVTSFL 361
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.59
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -2
Query: 516 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 436
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 28.3 bits (60), Expect = 0.78
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 346 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 233
CI GF ++ + +RTR + PS +LS + V+ FL
Sbjct: 325 CIAGFVVNELYTTRTRIIAPS-AFQTLSLSSVMVTSFL 361
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 27.1 bits (57), Expect = 1.8
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -2
Query: 84 PIKDCTSVDLQCRHHLPLSR 25
P K+C + L+C +H+P SR
Sbjct: 46 PCKNCKAGKLECTYHMPSSR 65
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.2 bits (55), Expect = 3.2
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +3
Query: 264 DKESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIV 428
D ES H EG F + E MK+ALN ++ +S ++ I FD I D V
Sbjct: 985 DYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSV 1044
Query: 429 NIQAFDYY 452
++Y
Sbjct: 1045 EDILTEFY 1052
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 4.2
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -3
Query: 428 DNVDQVYDAWDVKVNGAIYVWQ 363
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 25.4 bits (53), Expect = 5.5
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +3
Query: 93 QARTAFTNSALLLAEQYGFDGIDLSWQLPK 182
Q T F S+L +A G+D I L W L K
Sbjct: 531 QGVTEFVPSSLGVALAKGYDEIGLEWSLTK 560
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 7.3
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +3
Query: 387 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 482
Y D P I+ V +N+ D T + NP + D
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
L32|Schizosaccharomyces pombe|chr 2|||Manual
Length = 103
Score = 24.6 bits (51), Expect = 9.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +3
Query: 297 ALVREMKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINL 416
AL+R A++ K S LP+++ I+ PSI N+
Sbjct: 2 ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 24.6 bits (51), Expect = 9.6
Identities = 26/108 (24%), Positives = 45/108 (41%), Gaps = 4/108 (3%)
Frame = +3
Query: 123 LLLAEQYGFDGIDLSWQLPKRKPKKIRSSIGSFWHSIKKTFGTTPVDDKESEHREGFTAL 302
++ E YG DGI+LS +R + + I KT + D FT
Sbjct: 831 IIAKEMYGADGIELSPLAKERLETFTKQGYNNLPICIAKTQYSLSHDPDLKGAPTNFTVP 890
Query: 303 VREMKQALN---VKPNMQLVISVLPNV-NSSIYFDVPSIINLVDIVNI 434
+R+M+ + + P + IS +P + Y+++ I DIV +
Sbjct: 891 IRDMRLSAGAGFIYP-LAAAISTIPGLPTKPAYYNI-DIAENGDIVGL 936
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,117,470
Number of Sequences: 5004
Number of extensions: 41192
Number of successful extensions: 138
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 225926624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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