Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0008_C08
(461 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch... 134 8e-33
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 40 2e-04
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 29 0.46
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 27 1.1
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 27 1.8
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 27 1.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 2.4
SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces... 26 2.4
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 26 2.4
SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr ... 26 3.2
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 25 4.3
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 25 4.3
SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 25 5.6
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 25 7.4
>SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 341
Score = 134 bits (323), Expect = 8e-33
Identities = 72/151 (47%), Positives = 92/151 (60%)
Frame = +2
Query: 5 PKAIVAIITNPVNSMVPIASEVLKKGGVYDPARVLGVTTLDVVRAAAFIGEINGVDPRSV 184
P+A ++TNPVNS VPI + L++ GV+ P + GVTTLD VRA+ F ++ +
Sbjct: 138 PEAKYLVVTNPVNSTVPIFKKALERVGVHQPKHLFGVTTLDSVRASRFTSQVTNGKAELL 197
Query: 185 TIPVIGGHSGVTIIPVLSQSQPAVQLSDQGKIKALTERIQEAGTEVVKAKAGGGSATLSM 364
IPV+GGHSG TI+P+LSQ V+L+ + K AL RIQ G EVVKAKAG GSATLSM
Sbjct: 198 HIPVVGGHSGATIVPLLSQG--GVELTGE-KRDALIHRIQFGGDEVVKAKAGAGSATLSM 254
Query: 365 XXXXXXXXXXXXXXXKGESNVVECAYVKSDL 457
GES V EC +V+S L
Sbjct: 255 AYAGARMASSVLRALAGESGVEECTFVESPL 285
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 39.5 bits (88), Expect = 2e-04
Identities = 25/67 (37%), Positives = 37/67 (55%), Gaps = 1/67 (1%)
Frame = +2
Query: 11 AIVAIITNPVNSMVPIASEVLKKGGVYDPARVLGV-TTLDVVRAAAFIGEINGVDPRSVT 187
AI+ + TNPV+ + LK G + RV+G T +D R IG++ G+DP+SV
Sbjct: 130 AILLVATNPVDVLT---YATLKLTG-FPAERVIGSGTIIDTARFQYLIGKLYGLDPQSVN 185
Query: 188 IPVIGGH 208
+IG H
Sbjct: 186 ADIIGEH 192
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 28.7 bits (61), Expect = 0.46
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = -3
Query: 417 SPLSPRSTA-PASLAPAYAIDSVAEPPPALAFTTSVPA 307
SP S+A PA+++ ++ ++ PPP + T S PA
Sbjct: 885 SPFPGASSAQPAAMSRTSSVSTLPPPPPTASMTASAPA 922
>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 559
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/45 (28%), Positives = 26/45 (57%)
Frame = +2
Query: 14 IVAIITNPVNSMVPIASEVLKKGGVYDPARVLGVTTLDVVRAAAF 148
+VA ++ P+ + +AS+++ ++DP ++ T RAAAF
Sbjct: 307 LVAAVSAPIGIVSGVASKIMYGTAMWDPLQIANNWTSRGGRAAAF 351
>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
Sap49|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 26.6 bits (56), Expect = 1.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = -3
Query: 411 LSPRSTAPASLAPAYAIDSVAEPPPALAFTTSVP 310
++P+ST P +PA + A PA TS+P
Sbjct: 204 VTPQSTLPPGFSPATPAPTSAANTPATIAATSIP 237
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 26.6 bits (56), Expect = 1.8
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 158 INGVDPRSVTIPVIGGHSGVTIIPVLSQSQPAVQLSD 268
++G DP + PVI GH G I+ + VQ+ D
Sbjct: 57 LSGKDPEGL-FPVILGHEGAGIVESVGPQVTTVQVGD 92
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 2.4
Identities = 13/36 (36%), Positives = 18/36 (50%), Gaps = 2/36 (5%)
Frame = +2
Query: 92 DPARVLGVTTLDVVRAAAFIGEINGVD--PRSVTIP 193
D R LG+ D++R + E+NGV P S P
Sbjct: 835 DTLRTLGLKEGDIIRVMNHVNELNGVSTKPASAITP 870
>SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 565
Score = 26.2 bits (55), Expect = 2.4
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = -3
Query: 453 SDFTYAHSTTFDSPLSPRSTAPASLAPAYAIDSVAEPPPALAFTT 319
SDF + P+SP STAP+S P ++ S + P T
Sbjct: 378 SDFAHFRYQRRSRPVSPCSTAPSS--PTFSTRSFSPTPDVTPLVT 420
>SPBC4B4.04 |||translation initiation factor eIF2A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 576
Score = 26.2 bits (55), Expect = 2.4
Identities = 18/66 (27%), Positives = 36/66 (54%), Gaps = 5/66 (7%)
Frame = +2
Query: 122 LDVVRAAAFIGEINGVDPRSV--TIPVIGGH--SGVTIIPVLSQ-SQPAVQLSDQGKIKA 286
+DV+ + + +N R V PVI G+ + P + Q +QP ++SD+ KI++
Sbjct: 469 IDVMSSGSANKHVNSSRQRVVPGATPVIDGNKKNNKKTKPAVKQVAQPTAEVSDEKKIRS 528
Query: 287 LTERIQ 304
L ++++
Sbjct: 529 LCKKLR 534
>SPBPB8B6.02c |||urea transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 673
Score = 25.8 bits (54), Expect = 3.2
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -3
Query: 255 TAGWLCDSTGMMVTPECPPMT 193
TAGWL G+++TPE +T
Sbjct: 420 TAGWLLTFLGIILTPEVSAVT 440
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 25.4 bits (53), Expect = 4.3
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -3
Query: 411 LSPRSTAPASLAPAYAIDSVAEPPPALAFTTSVP 310
L+P S PA PA +S PP + + TT P
Sbjct: 400 LAPTSNVPAYSTPARPTESPPPPPISSSSTTPRP 433
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 25.4 bits (53), Expect = 4.3
Identities = 10/42 (23%), Positives = 21/42 (50%)
Frame = +2
Query: 158 INGVDPRSVTIPVIGGHSGVTIIPVLSQSQPAVQLSDQGKIK 283
+ G P +P+IGGH G ++ + +++ D+ +K
Sbjct: 53 LQGDWPLPAKMPLIGGHEGAGVVVKVGAGVTRLKIGDRVGVK 94
>SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.0 bits (52), Expect = 5.6
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = -3
Query: 432 STTFDSPLSPRSTAPASLAPAYAIDSVAEPPPALAFTTSVPASCILSVSALILP 271
S+ +P+SPR+ A SL P + S+ ++ F+T P LS SA I P
Sbjct: 417 SSLKSAPVSPRTAAAQSLLPNSVVSSI-NGMNSVNFSTISPPP--LSRSASISP 467
>SPAC343.11c |msc1||multi-copy suppressor of Chk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1588
Score = 24.6 bits (51), Expect = 7.4
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3
Query: 9 KLLSLLSPIQSTLWC 53
KLL+ LSP+++ WC
Sbjct: 427 KLLNTLSPLETFFWC 441
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.134 0.428
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,333,147
Number of Sequences: 5004
Number of extensions: 20885
Number of successful extensions: 100
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 174340060
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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