Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_N07
(675 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 343 1e-95
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 196 3e-51
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 194 1e-50
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 175 4e-45
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 31 0.20
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 2.5
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.3
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.3
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 5.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.6
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.6
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 25 10.0
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 10.0
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 343 bits (844), Expect = 1e-95
Identities = 153/222 (68%), Positives = 179/222 (80%)
Frame = +1
Query: 7 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 186
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 187 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 366
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 367 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 546
R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 547 EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 672
EPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 196 bits (477), Expect = 3e-51
Identities = 91/228 (39%), Positives = 134/228 (58%), Gaps = 6/228 (2%)
Frame = +1
Query: 7 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 168
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 169 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 348
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 349 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 528
SVL+ +R+ +++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 529 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 672
VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 194 bits (473), Expect = 1e-50
Identities = 91/224 (40%), Positives = 131/224 (58%), Gaps = 2/224 (0%)
Frame = +1
Query: 7 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 180
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 181 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 360
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 361 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 540
+R+ +++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 541 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 672
VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 175 bits (427), Expect = 4e-45
Identities = 81/224 (36%), Positives = 132/224 (58%), Gaps = 3/224 (1%)
Frame = +1
Query: 10 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 189
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 190 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 363
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 364 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 540
+ +E++ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 541 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTY 672
VV+PYN+ L++ +L N D +DN AL I L PT+
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 30.7 bits (66), Expect = 0.20
Identities = 29/87 (33%), Positives = 44/87 (50%), Gaps = 7/87 (8%)
Frame = +2
Query: 416 TLSE-AVPAPVWAHYLSQRSVKSTPTES*THTQSSP----RPKYQTPSSNPTMRLSQCTN 580
T+S+ AVP+ +A+ +S RSV S T+S T++SP K S T+ S TN
Sbjct: 643 TVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSPPFVNNTKPIVAKSPVTVTASSETN 702
Query: 581 --S*KTQTKHTVSITRHSTIFASARSN 655
S K K + ++ + +F RSN
Sbjct: 703 KKSQKINKKASPRVSLWTKLFGKFRSN 729
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 310 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 399
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 27.1 bits (57), Expect = 2.5
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +2
Query: 248 SDRYSALTTLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 424
++ Y +TL P+P+ T+ + S S +LT ++ PN V + N++ S
Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101
Query: 425 EAVPAPVWAHYLSQRSVKST 484
A P+ A + +R V +T
Sbjct: 102 TAPLNPIDACRVGERKVFTT 121
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 46 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 147
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 241 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 342
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.2 bits (55), Expect = 4.3
Identities = 28/117 (23%), Positives = 42/117 (35%), Gaps = 1/117 (0%)
Frame = +2
Query: 296 PVTTGPRDTTQRALS*STQFLT*SEKNPNHVIVYKDSNSHTLSEAVPAPVWAHYL-SQRS 472
PVT+ P TT S S + + + N+ I + T L +
Sbjct: 485 PVTSTPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTSTP 544
Query: 473 VKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSITRHSTIFAS 643
V STP + T S+ P TP ++ +S T T T T S ++ S
Sbjct: 545 VTSTPLATTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTTTNCTTSTSVLYTS 601
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 5.7
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +2
Query: 377 PNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSVKSTPTES 496
P VIV +DS S L+ +P P H L P+ S
Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQLQSFVPPQPPSSS 62
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.6
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 262 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 393
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.6
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 299 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 204
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 25.0 bits (52), Expect = 10.0
Identities = 16/62 (25%), Positives = 32/62 (51%)
Frame = +2
Query: 470 SVKSTPTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHTVSITRHSTIFASAR 649
S S+P+ S T T +SP + SS+ + S ++S + + + S + S+ +S+
Sbjct: 139 SSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 198
Query: 650 SN 655
S+
Sbjct: 199 SS 200
>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 530
Score = 25.0 bits (52), Expect = 10.0
Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
Frame = +2
Query: 431 VPAPVWAHYLSQRSVKST-PTES*THTQSSPRPKYQTPSSNPTMRLSQCTNS*KTQTKHT 607
V APVW++ S T T S S P ++ SS P ++ + T+ T
Sbjct: 121 VTAPVWSNTSVPVSTPETSATSSSEFFTSYPATSSESSSSYPASSTEVASSYSASSTEVT 180
Query: 608 VSITRHSTIFASARSNYLHP 667
S S + A++ S+Y+ P
Sbjct: 181 SSYPASSEV-ATSTSSYVAP 199
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.136 0.415
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,665,638
Number of Sequences: 5004
Number of extensions: 53218
Number of successful extensions: 192
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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