Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_M20
(624 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 338 3e-94
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 195 5e-51
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 182 4e-47
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 108 7e-25
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 103 2e-23
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 86 4e-18
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 48 5e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 2e-08
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 28 1.3
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 28 1.3
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 338 bits (832), Expect = 3e-94
Identities = 160/188 (85%), Positives = 171/188 (90%)
Frame = +3
Query: 3 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 182
TGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREI
Sbjct: 149 TGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREI 208
Query: 183 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLG 362
VRDIKEKLCYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG
Sbjct: 209 VRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALG 268
Query: 363 MESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTXXXX 542
+E+ GIHE YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS+
Sbjct: 269 LENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVK 328
Query: 543 XXAPPEKE 566
APPE++
Sbjct: 329 IVAPPERK 336
Score = 42.7 bits (96), Expect = 4e-05
Identities = 19/19 (100%), Positives = 19/19 (100%)
Frame = +1
Query: 568 YSVWIGGSILASLSTFQQM 624
YSVWIGGSILASLSTFQQM
Sbjct: 337 YSVWIGGSILASLSTFQQM 355
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 195 bits (475), Expect = 5e-51
Identities = 87/172 (50%), Positives = 123/172 (71%)
Frame = +3
Query: 3 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 182
+G+V+DSGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E
Sbjct: 149 SGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFET 208
Query: 183 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLG 362
VR++KEKLCYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G
Sbjct: 209 VREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVG 268
Query: 363 MESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL 518
E G+ E ++++I DVDIRK LY V+SGG++MY G+ R++KEI L
Sbjct: 269 SEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQL 320
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 182 bits (443), Expect = 4e-47
Identities = 82/187 (43%), Positives = 122/187 (65%)
Frame = +3
Query: 3 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 182
TGIVL+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EI
Sbjct: 154 TGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEI 213
Query: 183 VRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLG 362
VR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G
Sbjct: 214 VREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMG 273
Query: 363 MESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTXXXX 542
E+ G+H ++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++
Sbjct: 274 SEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVK 333
Query: 543 XXAPPEK 563
A PE+
Sbjct: 334 IYASPER 340
Score = 32.7 bits (71), Expect = 0.044
Identities = 12/19 (63%), Positives = 18/19 (94%)
Frame = +1
Query: 568 YSVWIGGSILASLSTFQQM 624
++ W+GGSILASLSTF+++
Sbjct: 342 HNAWLGGSILASLSTFRRL 360
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 108 bits (259), Expect = 7e-25
Identities = 64/176 (36%), Positives = 96/176 (54%), Gaps = 7/176 (3%)
Frame = +3
Query: 3 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 176
TG V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER
Sbjct: 180 TGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239
Query: 177 EIVRDIKEKLCYVALDFEQEMA--TAAASTSLEKSYELPDGQ--VITIGNERFRCPEALF 344
IKE+ CYV D +E + L+ + E G I +G ERF PE F
Sbjct: 240 -----IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFF 294
Query: 345 QPSFLGME-SCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEI 509
P + + E V N + +D+RK LY N V+SGG+T++ +R+Q+++
Sbjct: 295 NPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDL 350
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 103 bits (247), Expect = 2e-23
Identities = 63/193 (32%), Positives = 99/193 (51%), Gaps = 20/193 (10%)
Frame = +3
Query: 9 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 188
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216
Query: 189 DIKEKLCYVALDFEQEMATA--AASTSLEKSYELPD------------------GQVITI 308
+IKE +CYV+ +F+++M + LE Y LPD QV+ +
Sbjct: 217 EIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNL 276
Query: 309 GNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIA 488
NERF PE LF PS + + GI E V S+ +I+ L N V GG +PG
Sbjct: 277 SNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFH 336
Query: 489 DRMQKEITALAPS 527
R+ E+ +LAP+
Sbjct: 337 KRLSSELRSLAPA 349
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 85.8 bits (203), Expect = 4e-18
Identities = 48/132 (36%), Positives = 70/132 (53%), Gaps = 9/132 (6%)
Frame = +3
Query: 153 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 332
S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299
Query: 333 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 485
E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 486 ADRMQKEITALA 521
++R+Q E+ LA
Sbjct: 360 SERLQAEVQRLA 371
Score = 30.3 bits (65), Expect = 0.24
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = +1
Query: 571 SVWIGGSILASLSTFQQM 624
+VW GGSILASL FQ +
Sbjct: 390 AVWFGGSILASLDNFQHL 407
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 48.0 bits (109), Expect(2) = 5e-11
Identities = 21/52 (40%), Positives = 32/52 (61%)
Frame = +3
Query: 375 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPST 530
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P +
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS 377
Score = 34.3 bits (75), Expect(2) = 5e-11
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +3
Query: 246 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 350
++ +T E +E PDG + G ER++ PE LF P
Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288
Score = 32.3 bits (70), Expect = 0.059
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +1
Query: 568 YSVWIGGSILASLSTFQQM 624
Y+ W+GGSIL+SL TF Q+
Sbjct: 392 YASWLGGSILSSLGTFHQL 410
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 54.0 bits (124), Expect = 2e-08
Identities = 21/70 (30%), Positives = 45/70 (64%)
Frame = +3
Query: 315 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 494
E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 495 MQKEITALAP 524
+++E+T++ P
Sbjct: 654 IKRELTSIMP 663
Score = 41.5 bits (93), Expect = 1e-04
Identities = 23/90 (25%), Positives = 41/90 (45%)
Frame = +3
Query: 3 TGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 182
+GIVL+ G+ SH +P+ G + R+ G + YL+K+ + SF
Sbjct: 166 SGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQ 225
Query: 183 VRDIKEKLCYVALDFEQEMATAAASTSLEK 272
+ C+V+ D+ ++A A LE+
Sbjct: 226 AELLMHDHCHVSSDYTHDIAHALDRDILER 255
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 27.9 bits (59), Expect = 1.3
Identities = 12/53 (22%), Positives = 24/53 (45%)
Frame = +3
Query: 429 KDLYANTVMSGGTTMYPGIADRMQKEITALAPSTXXXXXXAPPEKEVLRMDRW 587
K+LY++ ++ GG +PG A +++ I + + PP + W
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAW 579
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/49 (24%), Positives = 26/49 (53%)
Frame = +3
Query: 12 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 158
V+D G + + EG +P++ ++++ G D+T MK+L + +
Sbjct: 295 VVDMGAQKTSISCVEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +1
Query: 19 TLVTVSLTPCPSTKVTPCPTPSS 87
T T + TP P+T TP PT +S
Sbjct: 935 TKATTTFTPTPTTTTTPTPTTTS 957
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 2/25 (8%)
Frame = +1
Query: 28 TVSLTPCPST--KVTPCPTPSSVWT 96
T S+TP P+T +TP PT +S T
Sbjct: 847 TSSITPTPTTTSSITPTPTTTSTTT 871
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 2.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 52 STKVTPCPTPSSVWTW 99
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,575,675
Number of Sequences: 5004
Number of extensions: 49346
Number of successful extensions: 178
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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