Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_G03
(715 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 109 3e-25
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 39 6e-04
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 30 0.38
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 27 3.5
SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 4.7
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 4.7
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.7
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 26 6.1
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 6.1
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 25 8.1
SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 25 8.1
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 109 bits (263), Expect = 3e-25
Identities = 86/261 (32%), Positives = 113/261 (43%), Gaps = 36/261 (13%)
Frame = +3
Query: 18 IRHEARFYALSRKF-KPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGET 194
++ EA +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ +M
Sbjct: 94 MKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGEMSNSI 152
Query: 195 PYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTLIVKPD 353
Y IMFGPD CG + VH IF +K + HL + T+LYTLIVKPD
Sbjct: 153 DYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTLIVKPD 211
Query: 354 NTYEVLIDNEKVESGELEADW--DFLPPKKIKDPE-----------------AKKPEDWX 476
T+EV I+ + V G L D+ LPP +I DPE A KP+DW
Sbjct: 212 QTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADWVDEPEIPDPNAVKPDDWD 271
Query: 477 XXXXXXXXXXXXXX----XXXXXXHIPDPDASKXXXXXXXXXXXXXXXXIDNPDYK---- 632
+IPDP+A K I NP
Sbjct: 272 EDAPRMIPDPDAVKPEDWLEDEPLYIPDPEAQKPEDWDDEEDGDWIPSEIINPKCIEGAG 331
Query: 633 -GVWAPKQIDNPAYKGPWIHP 692
G W P I NP Y+GPW P
Sbjct: 332 CGEWKPPMIRNPNYRGPWSPP 352
Score = 35.5 bits (78), Expect = 0.008
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 612 IDNPDYKGVWAPKQIDNPAYKGPW 683
I NP+Y+G W+P I NP + G W
Sbjct: 340 IRNPNYRGPWSPPMIPNPEFIGEW 363
Score = 29.1 bits (62), Expect = 0.66
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +3
Query: 612 IDNPDYKGVWAPKQIDNPAY 671
I NP++ G W P++I NP Y
Sbjct: 354 IPNPEFIGEWYPRKIPNPDY 373
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 39.1 bits (87), Expect = 6e-04
Identities = 34/121 (28%), Positives = 55/121 (45%), Gaps = 2/121 (1%)
Frame = +3
Query: 84 PLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDICGPGTKKVHVIFS 263
P+V+ F VK + CG Y+ + + + K++ E P I FG CG I S
Sbjct: 103 PIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKKCGMFDYISLSIIS 161
Query: 264 YKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGELEADWDFLPPKK 437
Y GK HL + T +YTL+++ + T + D V +G++ ++ P K
Sbjct: 162 YDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTGDVGTNFFHSPTKW 220
Query: 438 I 440
I
Sbjct: 221 I 221
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 29.9 bits (64), Expect = 0.38
Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = +3
Query: 261 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGELE-ADWDFLPPKK 437
S + K H KK +D Y+H+Y + P + + + ++ ES + E + D+LP
Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196
Query: 438 IKDPEAKKPED 470
+K + P+D
Sbjct: 197 MKQKKG-GPKD 206
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/54 (25%), Positives = 25/54 (46%)
Frame = +3
Query: 309 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPEAKKPED 470
D +Y L +L+ + N+YE + + + + W FL D E KK ++
Sbjct: 753 DIIYERLVSLLSRVPNSYEDTLKFSAINTLQSLLFWFFLRKDNPADEEKKKDDE 806
>SPAC23G3.05c |||regulator of G-protein signaling
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 343
Score = 26.2 bits (55), Expect = 4.7
Identities = 17/69 (24%), Positives = 33/69 (47%)
Frame = +3
Query: 144 LKVFDCRLDQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 323
+K+F + +K + +P+E+M P + P K H + ++R +DV T
Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204
Query: 324 HLYTLIVKP 350
+L ++KP
Sbjct: 205 YLLNFLLKP 213
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +3
Query: 273 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGELEADWDFLPPKKIKDPE 452
KNH ++ K+ V L + DN Y+ ++ + K + L+ D P +K +
Sbjct: 995 KNHALRIGNEFKEKVNERYSKLEIDIDNVYQRMLLHAKKKYAALQLDSQGKPNLDVKGLD 1054
Query: 453 AKKPE 467
K+ E
Sbjct: 1055 MKRRE 1059
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 390 ESGELEADWDFLPPKKIKDPEAKKPEDW 473
E L+ LP + K P AKKPED+
Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDY 184
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 6.1
Identities = 27/98 (27%), Positives = 46/98 (46%), Gaps = 7/98 (7%)
Frame = +3
Query: 132 GGGYLKVFDCRLDQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 299
G +LKV + + M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 300 R--CKDDVYTHLYTLIVKPDNTYEVLIDN-EKVESGEL 404
CK+D + LY+ D E L+++ E+V S EL
Sbjct: 674 LLFCKEDEF--LYS-----DQENEDLLESFERVISEEL 704
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 411 DWDFLPPKKIKDPEAKKPEDW 473
DWD +P ++ K EA KP DW
Sbjct: 209 DWDDVPWRE-KPLEAMKPRDW 228
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = -3
Query: 254 HVNLLCTRTTDIRPKHNLVGCLAMHVLLVQSTVEYLQVTASAVDVLFVL 108
H ++ T + +P H LV + + + QVT S + VLF +
Sbjct: 1908 HPDISLNMTPNFKPSHELVQLINSSPFYRKHIISVHQVTRSDLHVLFAI 1956
>SPBC19C2.11c |||mitochondrial outer membrane protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 415 GTSYLRRRSRIQKQRNLKIGTTNPQS 492
GTS RRR R++K+ +KI + +S
Sbjct: 340 GTSQFRRRERVKKRHVIKIHEASNKS 365
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,007,309
Number of Sequences: 5004
Number of extensions: 63708
Number of successful extensions: 188
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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