Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_E05
(721 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 46 5e-06
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 45 1e-05
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 43 4e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.51
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.51
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 29 0.88
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 28 1.5
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.0
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 27 2.0
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 26 4.7
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 26 6.2
SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Sch... 25 8.2
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 46.0 bits (104), Expect = 5e-06
Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
Frame = +1
Query: 220 EHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYGKGSSL-YPEDARARAL 387
E PE L LNP VPTL+D + +IWES AI+ YL +KY + P D
Sbjct: 40 EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99
Query: 388 VDQRLYF 408
V Q L+F
Sbjct: 100 VIQYLFF 106
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 44.8 bits (101), Expect = 1e-05
Identities = 46/174 (26%), Positives = 64/174 (36%), Gaps = 15/174 (8%)
Frame = +1
Query: 232 PEFLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYFD 411
P + KL+P P +VDDG++ ES AI+ +LV KYG ED + ++F
Sbjct: 40 PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWMHFS 99
Query: 412 IGTLYQ------------RFGDYFYPQVFAG-APADKEKLAKIEDALQL--LDKFLDGQK 546
+L F+ + K K E L L +D L +
Sbjct: 100 EASLMPFIWASHVLDLSVNMTPIFFRYIVRQFVNGIKSKYLSKETFLNLDYIDNHLASNE 159
Query: 547 YAAGPNXXXXXXXXXXXXXXFEASDIDFKKYPNIKRWYETVRSTAPGYQEANEK 708
Y AG + + K Y NIKRW V S P + A EK
Sbjct: 160 YFAGEQFTAADPQMCFPIFAAQRDYLSQKPYKNIKRWMRVV-SDRPACRIAAEK 212
>SPCC965.07c |gst2||glutathione S-transferase
Gst2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 43.2 bits (97), Expect = 4e-05
Identities = 43/126 (34%), Positives = 61/126 (48%), Gaps = 15/126 (11%)
Frame = +1
Query: 217 GEHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY--GKGSSLYPEDARAR 381
GE E L LNP VPTLVD + +IWES AI+ YL +KY + SL +D
Sbjct: 39 GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKYDTDRKISLSFDDPEYY 98
Query: 382 ALVDQRLYFDI---GTLYQRFG--DYFYPQVFAGAPAD-----KEKLAKIEDALQLLDKF 531
L+ Q L+F G ++ + G ++F+ + A K L +ED L+ D +
Sbjct: 99 KLI-QYLFFQASGQGVIWGQAGWFNFFHHEPVVSAVTRYRNEIKRVLGVLEDILKDRD-Y 156
Query: 532 LDGQKY 549
L KY
Sbjct: 157 LVANKY 162
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.5 bits (63), Expect = 0.51
Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = -2
Query: 567 QIRAGCVLLTIEEFVQKLESVLDLGQLLFVGRRSGKYLRIEV---ISESLVEGTNVKVQA 397
+I A V + I + KLE V+DL Q+ + R K +IE+ + E+L+E K A
Sbjct: 226 KIPAAAVQIPIGQ-EDKLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSA 284
Query: 396 LIDE 385
LI++
Sbjct: 285 LIEK 288
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.51
Identities = 39/138 (28%), Positives = 57/138 (41%), Gaps = 7/138 (5%)
Frame = +2
Query: 311 PSSLTWSTSTVKAAASTQKTLERG----RSSISACTLTLVPSTRDSEITSIRKYLPERLP 478
P + T STST AST T +S S T T +P T +S T + +P P
Sbjct: 360 PPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNS-TTPVTPTVP---P 415
Query: 479 TKRSWPRSRTLSSFWTNSSMVKSTQPALI*P*LT--*ASSQACPASKRPI-STSKNTLTL 649
T S P T ++ T++S+ ++ P P T +S + P + P+ ST T
Sbjct: 416 TSSSTP--LTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNC 473
Query: 650 KGGMKQSEAQRPVTRKPM 703
PVT P+
Sbjct: 474 TTSTSIPYTSTPVTSTPL 491
>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 28.7 bits (61), Expect = 0.88
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +2
Query: 401 CTLTLVPSTRDSEITSIRKYLPERLPTKRSWP 496
CT+ +P+T++ + R ++P R PTK++WP
Sbjct: 139 CTM-YIPATKEHPL---RIWVPRRSPTKQTWP 166
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 27.9 bits (59), Expect = 1.5
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +1
Query: 283 DGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYF 408
+ LSI ESR I + ++G S L+P D + A + RL F
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF 303
>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 386
Score = 27.5 bits (58), Expect = 2.0
Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
Frame = +2
Query: 314 SSLTWSTSTVKAAASTQKTLERGRSSISACTLTLVPSTRDSEITSIRK-----YLPERLP 478
SS+ W++++ + + K SS+ + + +PS+ ++ +S+ K YLP L
Sbjct: 62 SSVKWASTSFDSTVTAHKEETPYSSSLGSHDSSSLPSSTNNRYSSVLKELCNTYLPSILS 121
Query: 479 TKRSWPRSRTL 511
T S P R L
Sbjct: 122 TYGSLPIRRLL 132
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 454 QVFAGAPADKEKLAKIEDALQLLDKFLDGQKYA 552
Q+ +GAP E+LAK + L++ D D YA
Sbjct: 395 QMKSGAPCRSERLAKYNELLRIEDNLGDSAIYA 427
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 26.2 bits (55), Expect = 4.7
Identities = 8/26 (30%), Positives = 17/26 (65%)
Frame = +1
Query: 280 DDGLSIWESRAIITYLVNKYGKGSSL 357
+ + IW+ R ++ L+N +G G+S+
Sbjct: 6 EKAIEIWKIRRLVKQLINCHGNGTSM 31
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 6.2
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = +1
Query: 229 KPEFLKLNPQHTVPTLVDDGL---SIWESRAIITYLVNKY 339
KP+FL NPQ V + V +GL + E + YL+ +Y
Sbjct: 349 KPKFLDANPQSAVASRVLEGLIECAPAEKDLYLYYLIMRY 388
>SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 8.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 420 GTNVKVQALIDERPRSSVFWVEAAAFTVL 334
GT+ KV ID +S +F+ AAF +L
Sbjct: 263 GTDAKVFIWIDRNSKSVLFFAFLAAFAIL 291
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,690,561
Number of Sequences: 5004
Number of extensions: 50448
Number of successful extensions: 152
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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