Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_B12
(630 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 83 2e-16
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 73 1e-13
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 66 1e-11
At1g64030.1 68414.m07252 serpin family protein / serine protease... 65 3e-11
At2g35580.1 68415.m04357 serpin family protein / serine protease... 63 1e-10
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 57 1e-08
At2g14540.1 68415.m01628 serpin family protein / serine protease... 52 4e-07
At1g62170.1 68414.m07013 serpin family protein / serine protease... 52 4e-07
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 42 3e-04
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 40 0.002
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi... 31 0.48
At2g21870.2 68415.m02599 expressed protein 31 0.83
At2g21870.1 68415.m02598 expressed protein 31 0.83
At3g04960.1 68416.m00538 expressed protein low similarity to SP|... 28 4.4
At1g66020.1 68414.m07493 terpene synthase/cyclase family protein... 28 4.4
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R... 28 5.9
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 82.6 bits (195), Expect = 2e-16
Identities = 52/163 (31%), Positives = 83/163 (50%), Gaps = 6/163 (3%)
Frame = +1
Query: 88 TMANKIYVGNKYTLDEKFTSS-SRQYQSEVETIDF-SDTKKAADIINQWANEKTRGHIKS 261
++AN +++ ++L F Y++ +DF S + D +N WA T G IK
Sbjct: 28 SIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVHTNGLIKQ 87
Query: 262 PISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSL 435
+S D+ID ++ L N ++F+G W F+A+ T K DFH+ T VK P M +
Sbjct: 88 ILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLDGTSVKVPFMTNYEDQ 147
Query: 436 FYTENKELGARLVELPYKES--DFRMVVILPDKLEGLSSVLEK 558
+ G +++ LPY E F M + LP+ EGL+ +LEK
Sbjct: 148 YLRSYD--GFKVLRLPYIEDQRQFSMYIYLPNDKEGLAPLLEK 188
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 73.3 bits (172), Expect = 1e-13
Identities = 50/143 (34%), Positives = 75/143 (52%), Gaps = 6/143 (4%)
Frame = +1
Query: 160 YQSEVETIDFSDTKKAADI--INQWANEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQ 327
Y + +DF+ TK A I +N WA T G IK +SDD+I + L N ++F+
Sbjct: 118 YNATCNQVDFA-TKPAEVINEVNAWAEVHTNGLIKEILSDDSIKTIRESMLILANAVYFK 176
Query: 328 GHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSLFYTENKELGARLVELPYKES--DF 501
G W F+A T DFH+ T+VK P M + Y E + G +++ LPY E F
Sbjct: 177 GAWSKKFDAKLTKSYDFHLLDGTMVKVPFMTNYKKQ-YLEYYD-GFKVLRLPYVEDQRQF 234
Query: 502 RMVVILPDKLEGLSSVLEKAAEK 570
M + LP+ +GL ++LE+ + K
Sbjct: 235 AMYIYLPNDRDGLPTLLEEISSK 257
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 66.5 bits (155), Expect = 1e-11
Identities = 46/150 (30%), Positives = 74/150 (49%), Gaps = 9/150 (6%)
Frame = +1
Query: 160 YQSEVETIDFSDTK-KAADIINQWANEKTRGHIKSPISDDTIDP-----NAAAALFNVIF 321
Y++ +DF+ + D +N WA+ T G IK +S D D N+ L N ++
Sbjct: 115 YKASCSQVDFATKPVEVIDEVNIWADVHTNGLIKQILSRDCTDTIKEIRNSTLILANAVY 174
Query: 322 FQGHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSLFYTENKELGARLVELPYKES-- 495
F+ W F+A T DFH+ VK P M + Y + G +++ LPY E
Sbjct: 175 FKAAWSRKFDAKLTKDNDFHLLDGNTVKVPFMMSYKDQ-YLRGYD-GFQVLRLPYVEDKR 232
Query: 496 DFRMVVILPDKLEGLSSVLEK-AAEKGLIE 582
F M + LP+ +GL+++LEK + E G ++
Sbjct: 233 HFSMYIYLPNDKDGLAALLEKISTEPGFLD 262
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 65.3 bits (152), Expect = 3e-11
Identities = 47/167 (28%), Positives = 73/167 (43%), Gaps = 8/167 (4%)
Frame = +1
Query: 88 TMANKIYVGNKYTLDEKFTSSSRQYQSEVET-IDF-SDTKKAADIINQWANEKTRGHIKS 261
T AN +++ D KF + V +DF S+ ++ +N W T IK
Sbjct: 94 TAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWVEHHTNNLIKD 153
Query: 262 PISDDTIDPNAAAALFNVIFFQGHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSLFY 441
+ D ++ N + F+G W PF T DF++ + T V P M ++ +
Sbjct: 154 LLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVPFMSSYENQYV 213
Query: 442 TENKELGARLVELPYKE-SD-----FRMVVILPDKLEGLSSVLEKAA 564
G +++ LPY+ SD F M LPDK +GL +LEK A
Sbjct: 214 RAYD--GFKVLRLPYQRGSDDTNRKFSMYFYLPDKKDGLDDLLEKMA 258
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 63.3 bits (147), Expect = 1e-10
Identities = 49/175 (28%), Positives = 83/175 (47%), Gaps = 11/175 (6%)
Frame = +1
Query: 94 ANKIYVGNKYTLDEKFTSSS-RQYQSEVETIDFSDTKKAADI---INQWANEKTRGHIKS 261
AN +++ ++ F Y++ +DF KA ++ +N W ++T G I +
Sbjct: 95 ANGLWIEKTLNVEPSFKDLLLNSYKAAFNRVDFRT--KADEVNREVNSWVEKQTNGLITN 152
Query: 262 PISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSLF 438
+ + +F N +FF G W F+ S T DFH+ T V+ P M S
Sbjct: 153 LLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDGTKVRVPFM-TGASCR 211
Query: 439 YTENKELGARLVELPYKES-----DFRMVVILPDKLEGLSSVLEK-AAEKGLIED 585
YT E G +++ L Y+ F M + LPD+ +GL S+LE+ A+ +G ++D
Sbjct: 212 YTHVYE-GFKVINLQYRRGREDSRSFSMQIYLPDEKDGLPSMLERLASTRGFLKD 265
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 56.8 bits (131), Expect = 1e-08
Identities = 42/165 (25%), Positives = 73/165 (44%), Gaps = 8/165 (4%)
Frame = +1
Query: 88 TMANKIYVGNKYTLDEKFTSSSRQ-YQSEVETIDFSDTKKAADII---NQWANEKTRGHI 255
++AN ++ + F Y++ DF KA ++I N WA ++T G I
Sbjct: 93 SVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS--KAVEVIAEVNSWAEKETNGLI 150
Query: 256 KSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETIKKDFHVDSTTVVKKPTMHLLQSL 435
+ + + D N ++F+G W+ F+ S T + +FH+ V P M +
Sbjct: 151 TEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGNKVTAPFMTSKKKQ 210
Query: 436 FYTENKELGARLVELPYKES----DFRMVVILPDKLEGLSSVLEK 558
+ + G +++ LPY + F M LPD GLS +L+K
Sbjct: 211 YVSAYD--GFKVLGLPYLQGQDKRQFSMYFYLPDANNGLSDLLDK 253
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 51.6 bits (118), Expect = 4e-07
Identities = 35/140 (25%), Positives = 63/140 (45%), Gaps = 7/140 (5%)
Frame = +1
Query: 160 YQSEVETIDFSDTKKAADI-INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHW 336
+++ +DF + + +N WA+ T IK + ++ N ++F+G W
Sbjct: 147 FKASFAKVDFRHKAEEVRLDVNTWASRHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAW 206
Query: 337 HVPFNASETIKKDFHVDSTTVVKKPTMHLLQSLFYTENKELGARLVELPYKES------D 498
F+ S T K FH+ + V P M + F G +++ LPY++ +
Sbjct: 207 EKAFDKSMTRDKPFHLLNGKSVSVPFMRSYEKQFIEAYD--GFKVLRLPYRQGRDDTNRE 264
Query: 499 FRMVVILPDKLEGLSSVLEK 558
F M + LPDK L ++LE+
Sbjct: 265 FSMYLYLPDKKGELDNLLER 284
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 51.6 bits (118), Expect = 4e-07
Identities = 34/133 (25%), Positives = 62/133 (46%), Gaps = 7/133 (5%)
Frame = +1
Query: 181 IDF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNAS 357
+DF S ++ +N WA+ T G IK + ++ + ++F+G W ++ S
Sbjct: 190 VDFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKS 249
Query: 358 ETIKKDFHVDSTTVVKKPTMHLLQSLFYTENKELGARLVELPYKES------DFRMVVIL 519
T K F++ + T V P M + + G +++ LPY++ +F M + L
Sbjct: 250 MTKCKPFYLLNGTSVSVPFMSSFEKQYIAAYD--GFKVLRLPYRQGRDNTNRNFAMYIYL 307
Query: 520 PDKLEGLSSVLEK 558
PDK L +LE+
Sbjct: 308 PDKKGELDDLLER 320
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 42.3 bits (95), Expect = 3e-04
Identities = 31/120 (25%), Positives = 52/120 (43%), Gaps = 3/120 (2%)
Frame = +1
Query: 217 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETIKKDFHVDSTT 396
+N WA T G IK+ + ++ N ++F+G W F S TI K FH+ +
Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98
Query: 397 VVKKPTMHLLQSLF---YTENKELGARLVELPYKESDFRMVVILPDKLEGLSSVLEKAAE 567
V P M + + Y K L + YK++ + + + L L + E++AE
Sbjct: 99 QVLVPFMKSYERKYMKAYNGFKVLRILQYRVDYKDTSRQFSIDM--DLNVLIEIDEESAE 156
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 39.5 bits (88), Expect = 0.002
Identities = 23/92 (25%), Positives = 42/92 (45%)
Frame = +1
Query: 217 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETIKKDFHVDSTT 396
+N+WA++ T G I + ++ N ++F+G W F+ S T +FH
Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFHQGKE- 74
Query: 397 VVKKPTMHLLQSLFYTENKELGARLVELPYKE 492
V P M +S + G +++ LPY++
Sbjct: 75 -VHVPFMRSYESQYIMACD--GFKVLGLPYQQ 103
>At1g64010.1 68414.m07250 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 185
Score = 31.5 bits (68), Expect = 0.48
Identities = 22/73 (30%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
Frame = +1
Query: 361 TIKKDFHVDSTTVVKKPTMHLLQSLFYTENKELGARLVELPYKESD-----FRMVVILPD 525
T +DFH+ + T V M + Y E + G ++++LP+++ + F M LPD
Sbjct: 2 TKDRDFHLINGTSVSVSLMSSYKDQ-YIEAYD-GFKVLKLPFRQGNDTSRNFSMHFYLPD 59
Query: 526 KLEGLSSVLEKAA 564
+ +GL +++EK A
Sbjct: 60 EKDGLDNLVEKMA 72
>At2g21870.2 68415.m02599 expressed protein
Length = 220
Score = 30.7 bits (66), Expect = 0.83
Identities = 44/151 (29%), Positives = 67/151 (44%), Gaps = 10/151 (6%)
Frame = +1
Query: 85 FTMANKIYVGNKYTLDEKFTSSSRQYQSEVETIDFSDTKKAADIINQWANEKTRGHIKSP 264
F A I K TLD + ++ +QY + ++TI ++ AD+ + E R IK
Sbjct: 58 FQAAVDILRKEKITLDPEDPAAVKQYANVMKTI-----RQKADMFS----ESQR--IKHD 106
Query: 265 ISDDTID-PNAAAALFNVIFFQ---------GHWHVPFNASETIKKDFHVDSTTVVKKPT 414
I +T D P+A A L + + G + F A E ++KD KK
Sbjct: 107 IDTETQDIPDARAYLLKLQEIRTRRGLTDELGAEAMMFEALEKVEKDIKKPLLRSDKKG- 165
Query: 415 MHLLQSLFYTENKELGARLVELPYKESDFRM 507
M LL + F NK+LG R +LP E + +
Sbjct: 166 MDLLVAEFEKGNKKLGIRKEDLPKYEENLEL 196
>At2g21870.1 68415.m02598 expressed protein
Length = 240
Score = 30.7 bits (66), Expect = 0.83
Identities = 44/151 (29%), Positives = 67/151 (44%), Gaps = 10/151 (6%)
Frame = +1
Query: 85 FTMANKIYVGNKYTLDEKFTSSSRQYQSEVETIDFSDTKKAADIINQWANEKTRGHIKSP 264
F A I K TLD + ++ +QY + ++TI ++ AD+ + E R IK
Sbjct: 58 FQAAVDILRKEKITLDPEDPAAVKQYANVMKTI-----RQKADMFS----ESQR--IKHD 106
Query: 265 ISDDTID-PNAAAALFNVIFFQ---------GHWHVPFNASETIKKDFHVDSTTVVKKPT 414
I +T D P+A A L + + G + F A E ++KD KK
Sbjct: 107 IDTETQDIPDARAYLLKLQEIRTRRGLTDELGAEAMMFEALEKVEKDIKKPLLRSDKKG- 165
Query: 415 MHLLQSLFYTENKELGARLVELPYKESDFRM 507
M LL + F NK+LG R +LP E + +
Sbjct: 166 MDLLVAEFEKGNKKLGIRKEDLPKYEENLEL 196
>At3g04960.1 68416.m00538 expressed protein low similarity to
SP|P32380 NUF1 protein (Spindle poly body spacer protein
SPC110) {Saccharomyces cerevisiae}
Length = 556
Score = 28.3 bits (60), Expect = 4.4
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +1
Query: 490 ESDFRMVVILPDKLEGLSSVLEKAAEKGLIE 582
+SD + V ++EG +SV E+AA+ GL+E
Sbjct: 465 DSDRGIGVAYLGRVEGFTSVYERAAQNGLVE 495
>At1g66020.1 68414.m07493 terpene synthase/cyclase family protein
contains Pfam profile: PF01397: Terpene synthase family
Length = 598
Score = 28.3 bits (60), Expect = 4.4
Identities = 20/79 (25%), Positives = 33/79 (41%)
Frame = +1
Query: 106 YVGNKYTLDEKFTSSSRQYQSEVETIDFSDTKKAADIINQWANEKTRGHIKSPISDDTID 285
+ G+ E T ++ S E TK D I Q A T H++S + T
Sbjct: 176 FKGSNGNFKESLTGYAKGMLSLYEAAHLGTTK---DYILQEALSFTSSHLESLAACGTCP 232
Query: 286 PNAAAALFNVIFFQGHWHV 342
P+ + + NV+ HW++
Sbjct: 233 PHLSVHIQNVLSVPQHWNM 251
>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
(RPN1) contains an APC-complex (cyclosome) and
proteasome component repeat ( PS50248)
Length = 891
Score = 27.9 bits (59), Expect = 5.9
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = -1
Query: 234 ISPLINDVSCFFGIAEVNSFNLALVLTGRAREFLIQSIFISDVD 103
+SP++ND + S +L ++ G E + QSI + +D
Sbjct: 506 LSPILNDAKAPLDVIAFASLSLGMIYVGSCNEEVAQSIIFALMD 549
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,492,335
Number of Sequences: 28952
Number of extensions: 275508
Number of successful extensions: 818
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 811
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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