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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0007_B10
         (450 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,...    29   1.9  
At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine-...    28   3.4  
At3g57420.1 68416.m06393 expressed protein contains Pfam domain ...    27   5.9  
At3g52970.1 68416.m05839 cytochrome P450 family protein cytochro...    27   7.7  
At3g19630.1 68416.m02488 radical SAM domain-containing protein s...    27   7.7  
At1g33720.1 68414.m04169 cytochrome P450, putative similar to SP...    27   7.7  

>At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,
           putative / stearoyl-ACP desaturase, putative similar to
           Acyl-[acyl-carrier protein] desaturase from Spinacia
           oleracea SP|P28645, Olea europaea SP|Q43593; contains
           Pfam profile PF03405 Fatty acid desaturase
          Length = 396

 Score = 28.7 bits (61), Expect = 1.9
 Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 3/98 (3%)
 Frame = +1

Query: 118 PNHVSKSITISASWSIENNINHY*NETAVKIWILMKKQNWLLALSVPF-SPLNPTHQFEA 294
           P H+  S T      + N + H    T  K+ I    +NW     + +  P+  + Q + 
Sbjct: 36  PRHLQVSKTFRPIKEVSNQVTH--TITQEKLEIFKSMENWAQENLLSYLKPVETSWQPQ- 92

Query: 295 VIMFNVLYSAKDYDTFYKTTVYMKDRVNQ--DLYLYVL 402
               + L   KD D FY+    ++DR  +  D Y  VL
Sbjct: 93  ----DFLPETKDEDRFYEQVKELRDRTKEIPDDYFVVL 126


>At3g55400.1 68416.m06153 methionyl-tRNA synthetase /
           methionine--tRNA ligase / MetRS (cpMetRS) identical to
           methionyl-tRNA synthetase MEtRS [Arabidopsis thaliana]
           GI:2266985
          Length = 616

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 18/69 (26%), Positives = 32/69 (46%), Gaps = 3/69 (4%)
 Frame = +1

Query: 241 LALSVPFSPLNPTHQFEAVIM-FNVLYSAKD--YDTFYKTTVYMKDRVNQDLYLYVLSTL 411
           +A S   +  NP    + +   +  L+   D  YD F +TT    + + ++ Y  V +  
Sbjct: 122 IATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFYARVFANG 181

Query: 412 HIHRSDLEG 438
            I+R+D EG
Sbjct: 182 DIYRADYEG 190


>At3g57420.1 68416.m06393 expressed protein contains Pfam domain
           PF03385: Protein of unknown function, DUF288
          Length = 765

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 11/31 (35%), Positives = 16/31 (51%)
 Frame = +1

Query: 331 YDTFYKTTVYMKDRVNQDLYLYVLSTLHIHR 423
           Y   +KT V +  R N DLY+      HI++
Sbjct: 534 YGRIFKTVVILSSRKNSDLYVQEAKLDHIYK 564


>At3g52970.1 68416.m05839 cytochrome P450 family protein cytochrome
           P450 76A2, eggplant, PIR:S38534
          Length = 516

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = +1

Query: 136 SITISASWSIENNINHY*NETAVKIWILMKKQNWLLALSVPF 261
           S+  S  W++EN +N    +   +I I +KK   L A+ +P+
Sbjct: 472 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIPIPY 513


>At3g19630.1 68416.m02488 radical SAM domain-containing protein
           similar to florfenicol resistance protein
           [Staphylococcus sciuri] GI:9909980; contains Pfam
           profile PF04055: radical SAM domain protein
          Length = 372

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 13/45 (28%), Positives = 21/45 (46%)
 Frame = +1

Query: 253 VPFSPLNPTHQFEAVIMFNVLYSAKDYDTFYKTTVYMKDRVNQDL 387
           +PF+P+  T QFE   +  V    K     YK    ++  + QD+
Sbjct: 297 IPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDI 341


>At1g33720.1 68414.m04169 cytochrome P450, putative similar to
           SP|O64636 Cytochrome P450 76C1 (EC 1.14.-.-)
           {Arabidopsis thaliana}; contains Pfam profile PF00067:
           Cytochrome P450
          Length = 511

 Score = 26.6 bits (56), Expect = 7.7
 Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 5/40 (12%)
 Frame = -3

Query: 136 ISKRDLVRE----HGSIIR-KYLSAFSRTRLNHQFSLGHI 32
           I+ RD VRE    H  I+  +Y+S  +++  +H+FS+G I
Sbjct: 85  IASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWI 124


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,100,611
Number of Sequences: 28952
Number of extensions: 172473
Number of successful extensions: 381
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 381
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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