BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0007_B10 (450 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase,... 29 1.9 At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine-... 28 3.4 At3g57420.1 68416.m06393 expressed protein contains Pfam domain ... 27 5.9 At3g52970.1 68416.m05839 cytochrome P450 family protein cytochro... 27 7.7 At3g19630.1 68416.m02488 radical SAM domain-containing protein s... 27 7.7 At1g33720.1 68414.m04169 cytochrome P450, putative similar to SP... 27 7.7 >At3g02620.1 68416.m00253 acyl-[acyl-carrier-protein] desaturase, putative / stearoyl-ACP desaturase, putative similar to Acyl-[acyl-carrier protein] desaturase from Spinacia oleracea SP|P28645, Olea europaea SP|Q43593; contains Pfam profile PF03405 Fatty acid desaturase Length = 396 Score = 28.7 bits (61), Expect = 1.9 Identities = 25/98 (25%), Positives = 40/98 (40%), Gaps = 3/98 (3%) Frame = +1 Query: 118 PNHVSKSITISASWSIENNINHY*NETAVKIWILMKKQNWLLALSVPF-SPLNPTHQFEA 294 P H+ S T + N + H T K+ I +NW + + P+ + Q + Sbjct: 36 PRHLQVSKTFRPIKEVSNQVTH--TITQEKLEIFKSMENWAQENLLSYLKPVETSWQPQ- 92 Query: 295 VIMFNVLYSAKDYDTFYKTTVYMKDRVNQ--DLYLYVL 402 + L KD D FY+ ++DR + D Y VL Sbjct: 93 ----DFLPETKDEDRFYEQVKELRDRTKEIPDDYFVVL 126 >At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) identical to methionyl-tRNA synthetase MEtRS [Arabidopsis thaliana] GI:2266985 Length = 616 Score = 27.9 bits (59), Expect = 3.4 Identities = 18/69 (26%), Positives = 32/69 (46%), Gaps = 3/69 (4%) Frame = +1 Query: 241 LALSVPFSPLNPTHQFEAVIM-FNVLYSAKD--YDTFYKTTVYMKDRVNQDLYLYVLSTL 411 +A S + NP + + + L+ D YD F +TT + + ++ Y V + Sbjct: 122 IATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEFYARVFANG 181 Query: 412 HIHRSDLEG 438 I+R+D EG Sbjct: 182 DIYRADYEG 190 >At3g57420.1 68416.m06393 expressed protein contains Pfam domain PF03385: Protein of unknown function, DUF288 Length = 765 Score = 27.1 bits (57), Expect = 5.9 Identities = 11/31 (35%), Positives = 16/31 (51%) Frame = +1 Query: 331 YDTFYKTTVYMKDRVNQDLYLYVLSTLHIHR 423 Y +KT V + R N DLY+ HI++ Sbjct: 534 YGRIFKTVVILSSRKNSDLYVQEAKLDHIYK 564 >At3g52970.1 68416.m05839 cytochrome P450 family protein cytochrome P450 76A2, eggplant, PIR:S38534 Length = 516 Score = 26.6 bits (56), Expect = 7.7 Identities = 13/42 (30%), Positives = 23/42 (54%) Frame = +1 Query: 136 SITISASWSIENNINHY*NETAVKIWILMKKQNWLLALSVPF 261 S+ S W++EN +N + +I I +KK L A+ +P+ Sbjct: 472 SMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIPIPY 513 >At3g19630.1 68416.m02488 radical SAM domain-containing protein similar to florfenicol resistance protein [Staphylococcus sciuri] GI:9909980; contains Pfam profile PF04055: radical SAM domain protein Length = 372 Score = 26.6 bits (56), Expect = 7.7 Identities = 13/45 (28%), Positives = 21/45 (46%) Frame = +1 Query: 253 VPFSPLNPTHQFEAVIMFNVLYSAKDYDTFYKTTVYMKDRVNQDL 387 +PF+P+ T QFE + V K YK ++ + QD+ Sbjct: 297 IPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDI 341 >At1g33720.1 68414.m04169 cytochrome P450, putative similar to SP|O64636 Cytochrome P450 76C1 (EC 1.14.-.-) {Arabidopsis thaliana}; contains Pfam profile PF00067: Cytochrome P450 Length = 511 Score = 26.6 bits (56), Expect = 7.7 Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 5/40 (12%) Frame = -3 Query: 136 ISKRDLVRE----HGSIIR-KYLSAFSRTRLNHQFSLGHI 32 I+ RD VRE H I+ +Y+S +++ +H+FS+G I Sbjct: 85 IASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWI 124 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,100,611 Number of Sequences: 28952 Number of extensions: 172473 Number of successful extensions: 381 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 374 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 381 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 732537840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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