Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_B07
(605 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g37720.1 68418.m04541 RNA and export factor-binding protein, ... 33 0.11
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 31 0.59
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 31 0.59
At3g57630.2 68416.m06421 exostosin family protein contains Pfam ... 29 1.8
At3g57630.1 68416.m06420 exostosin family protein contains Pfam ... 29 1.8
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 29 3.2
At5g56900.2 68418.m07101 CwfJ-like family protein / zinc finger ... 28 4.2
At5g56900.1 68418.m07100 CwfJ-like family protein / zinc finger ... 28 4.2
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 28 4.2
At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR... 28 4.2
At3g59870.1 68416.m06681 expressed protein hypothetical protein ... 28 4.2
At5g57660.1 68418.m07205 zinc finger (B-box type) family protein... 28 5.5
At5g64550.1 68418.m08112 loricrin-related contains weak similari... 27 9.6
At5g24930.1 68418.m02952 zinc finger (B-box type) family protein... 27 9.6
At5g22010.1 68418.m02561 AAA-type ATPase family protein / BRCT d... 27 9.6
At4g29310.1 68417.m04190 expressed protein 27 9.6
>At5g37720.1 68418.m04541 RNA and export factor-binding protein,
putative transcriptional coactivator ALY, Mus musculus,
EMBL:MMU89876
Length = 288
Score = 33.5 bits (73), Expect = 0.11
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = -2
Query: 367 SRCTPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 227
SR P + R RGG RG GRGRG G + P+ +S
Sbjct: 223 SRRLPIHNQQGGGMRGGRGGFRARGRGNGGRGRGGGRGNGKKPVEKS 269
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 31.1 bits (67), Expect = 0.59
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +3
Query: 270 LPRPLGAVPRGAVPPRADRVLRDLQGNAGVQRERNVRLPAAH 395
LPRP+ P G +PP + + + G+ G+Q N ++ +H
Sbjct: 608 LPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSH 649
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 31.1 bits (67), Expect = 0.59
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = -1
Query: 287 SEWSRQRQRCRYTSQSDDQVSLKFVHTLRLESVSYGHSLMQLQIASPCSLIS 132
S++SR QRCR S ++ + +T + + HSL +++PC IS
Sbjct: 13 SQFSRSSQRCRLQSLTNLDFNFLGFNTKQTNLSASSHSLNNRSVSTPCFSIS 64
>At3g57630.2 68416.m06421 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 791
Score = 29.5 bits (63), Expect = 1.8
Identities = 17/68 (25%), Positives = 27/68 (39%), Gaps = 1/68 (1%)
Frame = +1
Query: 52 ESRDAPDNEINLDDPC-MKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 228
+ DA ++ + + C K C GR CEI C C+ +C R C
Sbjct: 240 DPEDAYAMKVKIKEECDCKYDCLWGRFCEIPVQ----CTCVNQCSGHGKCRGGFCQCDKG 295
Query: 229 TWSSDCEV 252
+ +DC +
Sbjct: 296 WFGTDCSI 303
>At3g57630.1 68416.m06420 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 793
Score = 29.5 bits (63), Expect = 1.8
Identities = 17/68 (25%), Positives = 27/68 (39%), Gaps = 1/68 (1%)
Frame = +1
Query: 52 ESRDAPDNEINLDDPC-MKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 228
+ DA ++ + + C K C GR CEI C C+ +C R C
Sbjct: 242 DPEDAYAMKVKIKEECDCKYDCLWGRFCEIPVQ----CTCVNQCSGHGKCRGGFCQCDKG 297
Query: 229 TWSSDCEV 252
+ +DC +
Sbjct: 298 WFGTDCSI 305
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.7 bits (61), Expect = 3.2
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 358 TPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSA 260
+PA C S ++ S+ T + PSGR R SA
Sbjct: 40 SPALRCASAASSSSSSATTAETSKPSGRNRRSA 72
>At5g56900.2 68418.m07101 CwfJ-like family protein / zinc finger
(CCCH-type) family protein contains Pfam domain,
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF04677: Protein similar to CwfJ C-terminus 1,
PF04676: Protein similar to CwfJ C-terminus 2
Length = 593
Score = 28.3 bits (60), Expect = 4.2
Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = -1
Query: 539 SRSHHFQMAALVQRRVRLDSASRSMRR*WGVSSRRSARSRIMLNSQSRMRRGKSDISFSL 360
S H FQ + +QR+ R ++A+RS + W S S S ++++ SL
Sbjct: 354 SYKHEFQDESSIQRKPRSENANRS-KECWFCLSSPSVESHLIVSVGESFYCALP--KGSL 410
Query: 359 HSGISLQVP*YSICTWWYCAPRHSSEWSRQRQRCR--YTSQSDDQVSLKFV 213
L +P + +P SE SR + R Y SQ +D V + V
Sbjct: 411 VEDHILIIPIEHLPNTLVLSPEVESELSRYQNGLRNCYKSQGNDAVFFELV 461
>At5g56900.1 68418.m07100 CwfJ-like family protein / zinc finger
(CCCH-type) family protein contains Pfam domain,
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF04677: Protein similar to CwfJ C-terminus 1,
PF04676: Protein similar to CwfJ C-terminus 2
Length = 404
Score = 28.3 bits (60), Expect = 4.2
Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = -1
Query: 539 SRSHHFQMAALVQRRVRLDSASRSMRR*WGVSSRRSARSRIMLNSQSRMRRGKSDISFSL 360
S H FQ + +QR+ R ++A+RS + W S S S ++++ SL
Sbjct: 165 SYKHEFQDESSIQRKPRSENANRS-KECWFCLSSPSVESHLIVSVGESFYCALP--KGSL 221
Query: 359 HSGISLQVP*YSICTWWYCAPRHSSEWSRQRQRCR--YTSQSDDQVSLKFV 213
L +P + +P SE SR + R Y SQ +D V + V
Sbjct: 222 VEDHILIIPIEHLPNTLVLSPEVESELSRYQNGLRNCYKSQGNDAVFFELV 272
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -2
Query: 334 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 227
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
Score = 27.9 bits (59), Expect = 5.5
Identities = 24/61 (39%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = -2
Query: 403 SRACAAGSLTFRSRCTPAFPCKSRS-TRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 227
SR+ + G +SR P +SRS +RS +P+ A S R R A T RSP +RS
Sbjct: 199 SRSPSRGRSYSKSRSRGRSPSRSRSRSRSRSKSRSPK--AKSLR-RSPAKSTSRSPRSRS 255
Query: 226 R 224
R
Sbjct: 256 R 256
>At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 178
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -2
Query: 334 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 227
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
>At3g59870.1 68416.m06681 expressed protein hypothetical protein
F6E13.7 - Arabidopsis thaliana, PIR:T00674
Length = 288
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -1
Query: 569 RDTNXSLSCASRSHHFQMAALVQRRVRLDSASRSM 465
R ++ S SC+S+S FQ+ ++ +RR R S S M
Sbjct: 33 RRSSSSTSCSSQSQSFQLFSVRRRRSRSSSPSIPM 67
>At5g57660.1 68418.m07205 zinc finger (B-box type) family protein
contains Pfam domain, PF00643: B-box zinc finger
Length = 355
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 475 EAESNLTRRWTNAAIWKWCDLDAHDNDXFVSRHELFPI 588
+A + +T + AA+ CD D H + SRHE P+
Sbjct: 66 QAPAAVTCKADAAALCVSCDADIHSANPLASRHERVPV 103
>At5g64550.1 68418.m08112 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 634
Score = 27.1 bits (57), Expect = 9.6
Identities = 11/37 (29%), Positives = 18/37 (48%), Gaps = 3/37 (8%)
Frame = +1
Query: 82 NLDDPCMKVHC---SAGRVCEINEHGEAICNCIKECP 183
N D + + C SAGR+ + H +C+ + CP
Sbjct: 21 NFGDTALSLKCLGSSAGRLIGSSHHNHKLCSDVSNCP 57
>At5g24930.1 68418.m02952 zinc finger (B-box type) family protein
similar to CONSTANS-like protein 1 GI:4091804 from
[Malus x domestica]
Length = 406
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +1
Query: 475 EAESNLTRRWTNAAIWKWCDLDAHDNDXFVSRHELFPI 588
+A +++T + AA+ CD D H + RHE P+
Sbjct: 98 QAPAHVTCKADAAALCVTCDRDIHSANPLARRHERVPV 135
>At5g22010.1 68418.m02561 AAA-type ATPase family protein / BRCT
domain-containing protein contains Pfam profiles:
PF00533 BRCA1 C Terminus (BRCT) domain, PF00004 ATPase
family associated with various cellular activities (AAA)
Length = 956
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 334 RSTRSARGGTAPRGTAPSGRGRGSAVG 254
+S RGG A G + GRGRG G
Sbjct: 154 KSAGRGRGGRAAPGASTGGRGRGGGRG 180
>At4g29310.1 68417.m04190 expressed protein
Length = 424
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -2
Query: 355 PAFPCKSRSTRSARGGTAPRGTAPSGRG 272
P F CK S R+ R + P G S RG
Sbjct: 186 PVFSCKFSSDRNGRSRSLPSGFTYSSRG 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,225,692
Number of Sequences: 28952
Number of extensions: 254507
Number of successful extensions: 987
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 987
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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