Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_A20
(500 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60140.1 68416.m06715 glycosyl hydrolase family 1 protein con... 33 0.082
At3g07050.1 68416.m00837 GTP-binding family protein contains Pfa... 29 2.3
At3g59980.1 68416.m06696 tRNA-binding region domain-containing p... 28 4.1
At2g27570.1 68415.m03340 sulfotransferase family protein similar... 27 5.4
At5g60970.1 68418.m07648 TCP family transcription factor, putati... 27 7.1
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 27 7.1
At2g44460.1 68415.m05528 glycosyl hydrolase family 1 protein con... 27 7.1
At3g32904.1 68416.m04164 hypothetical protein 27 9.4
At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A... 27 9.4
>At3g60140.1 68416.m06715 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to Cyanogenic Beta-Glucosidase
(GI:1311386)(pdb:1CBG) [Trifolium Repens]; identical
beta-glucosidase GI:10834547
Length = 577
Score = 33.5 bits (73), Expect = 0.082
Identities = 13/42 (30%), Positives = 26/42 (61%)
Frame = +1
Query: 136 IRYKRQIENNANLFIDKNGWNKSQDGNRPEWIPIQNGYRIQY 261
+ Y ++ NN ++I +NG N + DG +P +++ +RI+Y
Sbjct: 394 LNYIKERYNNMPVYIKENGINDNDDGTKPREEIVKDTFRIEY 435
>At3g07050.1 68416.m00837 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 582
Score = 28.7 bits (61), Expect = 2.3
Identities = 15/38 (39%), Positives = 26/38 (68%), Gaps = 2/38 (5%)
Frame = +2
Query: 302 LIKCSSQTKH-RYQVKGKHQLLALV-EHHDKQLRNARK 409
++K S ++K R +K KH++L V EHH K+ ++A+K
Sbjct: 1 MVKRSKKSKSKRVTLKQKHKVLKKVKEHHKKKAKDAKK 38
>At3g59980.1 68416.m06696 tRNA-binding region domain-containing
protein similar to SP|O54873 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Cricetulus griseus};
contains Pfam profile PF01588: Putative tRNA binding
domain
Length = 273
Score = 27.9 bits (59), Expect = 4.1
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = -1
Query: 440 RGCNLVLKSNSSAHFSIVCRDVPPVLGVDVCP*PGNGVWFGN 315
+ C ++L ++ +AH ++ VPP V PG+ VWFGN
Sbjct: 176 KSCGMLLAASDAAHENVELL-VPPEGSV-----PGDRVWFGN 211
>At2g27570.1 68415.m03340 sulfotransferase family protein similar to
steroid sulfotransferase from [Brassica napus]
GI:3420008, GI:3420006; contains Pfam profile PF00685:
Sulfotransferase domain
Length = 273
Score = 27.5 bits (58), Expect = 5.4
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -1
Query: 230 IHSGRFPS*DLFHPFLSINKFALFS 156
+H + PS D HP LS N LFS
Sbjct: 88 VHRSKHPSHDHHHPLLSNNPHVLFS 112
>At5g60970.1 68418.m07648 TCP family transcription factor, putative
putative basic helix-loop-helix DNA binding protein
TCP2, Arabidopsis thaliana, EMBL:AF072691
Length = 360
Score = 27.1 bits (57), Expect = 7.1
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +1
Query: 178 IDKNGWNKSQDGNRPEWIPIQNGYRIQYPLDNNYN 282
+DK W K+ + + + ++ Q+PL N YN
Sbjct: 192 LDKGKWIKNDENSNQDHQGFNTNHQQQFPLTNPYN 226
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein similar
to alpha-mannosidase II SP:P27046 from [Mus musculus]
Length = 1173
Score = 27.1 bits (57), Expect = 7.1
Identities = 14/44 (31%), Positives = 23/44 (52%)
Frame = +1
Query: 136 IRYKRQIENNANLFIDKNGWNKSQDGNRPEWIPIQNGYRIQYPL 267
+RYK ++N + D NG+ S+ + IP+Q Y YP+
Sbjct: 883 VRYKTDVDNKKVFYSDLNGFQMSRRETYDK-IPLQGNY---YPM 922
>At2g44460.1 68415.m05528 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to beta-glucosidase 1 (GI:12043529) [Arabidopsis
thaliana]
Length = 582
Score = 27.1 bits (57), Expect = 7.1
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +1
Query: 136 IRYKRQIENNANLFIDKNGWNKSQDGNRPEWIPIQNGYRIQYPLDN 273
+ Y + NN ++I +NG N DG + + + +RI Y D+
Sbjct: 396 LNYIKDKYNNPIVYIKENGINDYDDGTKSREEILNDTFRISYHEDH 441
>At3g32904.1 68416.m04164 hypothetical protein
Length = 330
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = +2
Query: 317 SQTKHRYQVKGKHQLLALVEHHDKQLRNARKNCFSTPDYN 436
S+ K ++ + L E + L+ R NCF DYN
Sbjct: 160 SRRKQSFETTLTDTITGLREFQRQSLQQMRPNCFDKDDYN 199
>At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit A (RCN1) identical to
phosphoprotein phosphatase 2A, regulatory subunit A
GI:1262171 from [Arabidopsis thaliana]
Length = 588
Score = 26.6 bits (56), Expect = 9.4
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -1
Query: 461 FMIAYHTRGCNLVLKSNSSAHFSIVCRDVPPVL 363
F +AY +GC VLK+ A +S +C+D P++
Sbjct: 149 FHVAY--QGCTDVLKTELRATYSQLCKDDMPMV 179
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,355,528
Number of Sequences: 28952
Number of extensions: 235221
Number of successful extensions: 541
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 541
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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