Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0007_A08
(585 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 34 0.061
At2g21410.1 68415.m02548 vacuolar proton ATPase, putative simila... 30 0.99
At4g39080.1 68417.m05534 vacuolar proton ATPase, putative simila... 29 1.7
At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 3.0
At1g70900.1 68414.m08181 expressed protein 29 3.0
At1g23110.1 68414.m02889 hypothetical protein 29 3.0
At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 5.3
At5g18840.1 68418.m02239 sugar transporter, putative similar to ... 28 5.3
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 5.3
At4g01290.1 68417.m00170 expressed protein 28 5.3
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 5.3
At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 7.0
At4g30550.1 68417.m04336 glutamine amidotransferase class-I doma... 27 7.0
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 7.0
At2g20170.1 68415.m02358 hypothetical protein and grail contain... 27 7.0
At2g16530.2 68415.m01896 3-oxo-5-alpha-steroid 4-dehydrogenase f... 27 7.0
At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 9.2
At5g13460.1 68418.m01549 calmodulin-binding family protein low s... 27 9.2
At4g11120.1 68417.m01804 translation elongation factor Ts (EF-Ts... 27 9.2
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 27 9.2
At3g06890.1 68416.m00818 expressed protein 27 9.2
At2g32830.1 68415.m04019 inorganic phosphate transporter (PHT5) ... 27 9.2
At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro... 27 9.2
At2g23990.1 68415.m02865 plastocyanin-like domain-containing pro... 27 9.2
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 34.3 bits (75), Expect = 0.061
Identities = 27/114 (23%), Positives = 53/114 (46%), Gaps = 3/114 (2%)
Frame = +3
Query: 168 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 347
NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + +
Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90
Query: 348 MANKIYVGNKYTLDEKF-TITVRQYQSEVETIDFSDTK--KAADIINQWANEKT 500
A+ +++ L F + Y++ +DF+ TK + D +N WA+ T
Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFA-TKPVEVIDEVNIWADVHT 143
>At2g21410.1 68415.m02548 vacuolar proton ATPase, putative similar
to vacuolar proton ATPase 100-kDa subunit from
Dictyostelium discoideum P|1384136|gb|AAB49621
Length = 821
Score = 30.3 bits (65), Expect = 0.99
Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +1
Query: 388 MRNSRSLSVSTKAKLKLLTSAIPKKQLTSLISGLMRRPRGHI---KSPISDDTIDPNA 552
++ +S+ + + KL LT +P+++ L R RG+I +S I + +DPN+
Sbjct: 178 LQEEKSVDPTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIRQSVIEESVVDPNS 235
>At4g39080.1 68417.m05534 vacuolar proton ATPase, putative similar
to Swiss-Prot:Q93050 vacuolar proton translocating
ATPase 116 kDa subunit A isoform 1 (Clathrin-coated
vesicle/synaptic vesicle proton pump 116 kDa subunit,
Vacuolar proton pump subunit 1, Vacuolar adenosine
triphosphatase subunit Ac116) [Homo sapiens]
Length = 821
Score = 29.5 bits (63), Expect = 1.7
Identities = 16/58 (27%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +1
Query: 388 MRNSRSLSVSTKAKLKLLTSAIPKKQLTSLISGLMRRPRGHI---KSPISDDTIDPNA 552
++ +S+ + + KL LT +P+++ L R RG+I ++ I + IDPN+
Sbjct: 177 LQEEKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNS 234
>At3g02280.1 68416.m00209 flavodoxin family protein low similarity
to SP|Q05001 NADPH-cytochrome P450 reductase (EC
1.6.2.4) {Catharanthus roseus}, similar to
NADPH-dependent FMN and FAD containing oxidoreductase
[Homo sapiens] GI:6694369; contains Pfam profiles
PF00258: flavodoxin, PF00667: FAD binding domain,
PF00175: Oxidoreductase NAD-binding domain
Length = 623
Score = 28.7 bits (61), Expect = 3.0
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%)
Frame = +3
Query: 234 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 395
G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K
Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182
Query: 396 FTITV-RQYQSEVETIDFSD 452
+ I +Q + E + + SD
Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202
>At1g70900.1 68414.m08181 expressed protein
Length = 244
Score = 28.7 bits (61), Expect = 3.0
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +3
Query: 165 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 344
K +S +GV + LY + SR + K+L DY+ +A I L++ E NP +
Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157
Query: 345 TMANKI 362
A+ +
Sbjct: 158 MAASAL 163
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 28.7 bits (61), Expect = 3.0
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +3
Query: 165 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 344
K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP +
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 345 TMANKI 362
A+ +
Sbjct: 175 MAASAL 180
>At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK16) mitogen-activated protein
kinase (MAPK)(AtMPK16), PMID:12119167; similar to
ATMPK9, Arabidopsis thaliana, EMBL:AB038694
Length = 567
Score = 27.9 bits (59), Expect = 5.3
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 228 GEGSRAEIDKFLGNGDYSGVANPY 299
GEGSR I++ +G G Y V + Y
Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43
>At5g18840.1 68418.m02239 sugar transporter, putative similar to
ERD6 protein {Arabidopsis thaliana} GI:3123712,
sugar-porter family protein 1 [Arabidopsis thaliana]
GI:14585699; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 482
Score = 27.9 bits (59), Expect = 5.3
Identities = 21/86 (24%), Positives = 37/86 (43%), Gaps = 2/86 (2%)
Frame = -2
Query: 572 TLKSAAAAFGSMVSSEIGDLICPRGLLISPLINDVSCFFGIAEVNSFNFALVLTDSDREF 393
TL GS VS IG LI + L ++ L + FG+ + L ++EF
Sbjct: 173 TLNQLMIVIGSSVSFLIGSLISWKTLALTGLAPCIVLLFGLCFIPESPRWLAKAGHEKEF 232
Query: 392 --LIQSIFISDVDFIGHSEVIRIHLR 321
+Q + D D ++ I++ ++
Sbjct: 233 RVALQKLRGKDADITNEADGIQVSIQ 258
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 27.9 bits (59), Expect = 5.3
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -3
Query: 217 LYRSISIITPNGEDTTFLSFDDVYVSLRN 131
LYR+ +++ P G+D ++S D+ RN
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 27.9 bits (59), Expect = 5.3
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%)
Frame = +1
Query: 352 PIKSTSEINILWMRNSRSLSVSTKAKLKLLT----SAIPKKQLTSLISGLMRRP 501
P+ + + + M R SV +A LL+ S+ PK Q T L+S RRP
Sbjct: 584 PVDQDTSVPSVKMTKQRKTSVDDQASQHLLSLLQRSSDPKSQDTQLLSATERRP 637
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 27.9 bits (59), Expect = 5.3
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +3
Query: 168 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 347
+VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337
Query: 348 MANKIYVGNKYTLDEKF 398
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 444
Score = 27.5 bits (58), Expect = 7.0
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +3
Query: 162 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 338
DKNV S LG + +L +G + + GDY G+ Y++ T M D
Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188
Query: 339 Y 341
Y
Sbjct: 189 Y 189
>At4g30550.1 68417.m04336 glutamine amidotransferase class-I
domain-containing protein similar to defense-related
protein [Brassica carinata] GI:14009290, component of
aniline dioxygenase (GMP synthase like protein) -
Acinetobacter sp.,PID:d1013698; contains Pfam profile
PF00117: glutamine amidotransferase class-I
Length = 249
Score = 27.5 bits (58), Expect = 7.0
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 8/60 (13%)
Frame = +3
Query: 27 LLAVIAVCNVRAFYSSDH--------EYNRTALGDAIDKTSLQFLKETYTSSKDKNVVSS 182
LLA VCNV F DH EYN+ L + +D+ L E + K K+ + +
Sbjct: 172 LLASSEVCNVEMFSIGDHFFCIQGHPEYNKEILFEIVDRVLNMKLMEQEFADKAKSTMET 231
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 27.5 bits (58), Expect = 7.0
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +3
Query: 252 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 374
D++L Y P I KT S P+ N++Y+GN
Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268
>At2g20170.1 68415.m02358 hypothetical protein and grail contains
Pfam profile PF03080: Arabidopsis proteins of unknown
function
Length = 401
Score = 27.5 bits (58), Expect = 7.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 90 RTALGDAIDKTSLQFLKETYTSSKDKNVVS 179
RT L D I L++L YT+SKDK+ ++
Sbjct: 121 RTTLEDLIQIQRLKYLGVKYTTSKDKDFLN 150
>At2g16530.2 68415.m01896 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to Arabidopsis thaliana DET2
[gi:1280611]; contains Pfam 3-oxo-5-alpha-steroid
4-dehydrogenase domain PF02544
Length = 342
Score = 27.5 bits (58), Expect = 7.0
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +1
Query: 370 EINILWMRNSRSLSVSTKAKLKLLTSAIPKKQLTSLISGLMR-RPRGHIKSPISDDTIDP 546
E+ I+W+ ++V + L L+ ++IP +L S ++ RG I P S TI P
Sbjct: 3 ELEIVWLVRGAWITVWIVSILPLVIASIPTSKLNSFRELVLSFAGRGKILHPSSQFTI-P 61
Query: 547 NAAAALFNVI 576
A F VI
Sbjct: 62 QKCFAHFYVI 71
>At5g59920.1 68418.m07514 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 710
Score = 27.1 bits (57), Expect = 9.2
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +2
Query: 83 VQQDSLRRCH*QNFFAVS*GNVYIIEGQECCVFTIRRDDAN 205
V D RRC V G++Y+ E +ECC F + AN
Sbjct: 384 VIHDERRRCE-ACIHPVQFGSIYVCEEEECC-FVLHEKCAN 422
>At5g13460.1 68418.m01549 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 443
Score = 27.1 bits (57), Expect = 9.2
Identities = 21/60 (35%), Positives = 26/60 (43%)
Frame = +3
Query: 375 KYTLDEKFTITVRQYQSEVETIDFSDTKKAADIINQWANEKTPRTY*ISNL**HHRSECS 554
KY LDE + T E+E +DFS K D KTPR L +HR + S
Sbjct: 255 KYWLDE-WVDTQLTKSKELEDLDFSSKTKPKDETLNEKQLKTPRNSSPRRLVNNHRRQVS 313
>At4g11120.1 68417.m01804 translation elongation factor Ts (EF-Ts),
putative similar to ethylene-responsive elongation
factor EF-Ts precursor [Lycopersicon esculentum]
GI:5669636; contains Pfam profile PF00889: Elongation
factor TS
Length = 395
Score = 27.1 bits (57), Expect = 9.2
Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 1/56 (1%)
Frame = +3
Query: 339 YFTMANKIYVGNKY-TLDEKFTITVRQYQSEVETIDFSDTKKAADIINQWANEKTP 503
Y++++++ GN+Y T+ KF T+ QY+S V + S + + I +++E P
Sbjct: 15 YYSVSSRFSSGNEYSTVASKFE-TLSQYKSSVPSGYTSLVRGFGNFIRSFSSEAPP 69
>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
contains Pfam profile PF04406: Type IIB DNA
topoisomerase; identical to cDNA putative topoisomerase
VIA (SPO11 gene 1) GI:7270974
Length = 362
Score = 27.1 bits (57), Expect = 9.2
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +3
Query: 105 DAIDKTSLQFLKETYTSSKDKNVVSSP 185
+A+ SL FL E Y SK + VSSP
Sbjct: 336 EALSVHSLSFLSEVYIPSKIRREVSSP 362
>At3g06890.1 68416.m00818 expressed protein
Length = 128
Score = 27.1 bits (57), Expect = 9.2
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -2
Query: 545 GSMVSSEIGDLICPRGLLISPLINDVSCFFGIAEVNSFNFALVLT 411
G ++ I +L+ P GLL+ P+I ++ F +E SF A+ T
Sbjct: 42 GEAITDAIAELLSPVGLLLLPIILLLTIQFLSSERGSFVSAIFST 86
>At2g32830.1 68415.m04019 inorganic phosphate transporter (PHT5)
identical to inorganic phosphate transporter (PHT5)
[Arabidopsis thaliana] GI:2780345
Length = 542
Score = 27.1 bits (57), Expect = 9.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 472 SLISGLMRRPRGHIKSPISDDTIDPNAAAALFNVIF 579
SL++ L+ R H+ S T+ PN AAA+ V F
Sbjct: 43 SLVTKLLGRIYYHVDSSKKPGTLPPNVAAAVNGVAF 78
>At2g23990.2 68415.m02866 plastocyanin-like domain-containing
protein
Length = 226
Score = 27.1 bits (57), Expect = 9.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 274 SPLPKNLSISARLPSPAPALYRSISIITPNG 182
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 181 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 210
>At2g23990.1 68415.m02865 plastocyanin-like domain-containing
protein
Length = 207
Score = 27.1 bits (57), Expect = 9.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 274 SPLPKNLSISARLPSPAPALYRSISIITPNG 182
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 162 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,695,966
Number of Sequences: 28952
Number of extensions: 265100
Number of successful extensions: 817
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 817
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1151426952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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