Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0006_P20
         (446 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c...    42   3e-05
SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase |Schizosacchar...    40   1e-04
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc...    28   0.75 
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    27   1.7  
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo...    25   4.0  
SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch...    25   5.3  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    25   7.0  
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|...    25   7.0  
SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomy...    24   9.3  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    24   9.3  

>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 429

 Score = 42.3 bits (95), Expect = 3e-05
 Identities = 23/57 (40%), Positives = 35/57 (61%), Gaps = 1/57 (1%)
 Frame = +3

Query: 276 LAALEGGKHALVFASGLGATTTIASLLSK-GDHILSSDDVYGGTNRLLRQVVSRMGI 443
           +AALE G  A+  +SG  A     + L+K GD+I+S+  +YGGT  L +  + R+GI
Sbjct: 72  IAALEHGAAAIATSSGTSALFMALTTLAKAGDNIVSTSYLYGGTYNLFKVTLPRLGI 128



 Score = 29.5 bits (63), Expect = 0.25
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 8/64 (12%)
 Frame = +2

Query: 104 FATQAIHAGQDPDKWDSAAVVAPIVTSTTF--------KQTAPTVHNGYEYGRSGNPTRN 259
           F T  +HAGQ+PD   S+  V PI  +T++         +       GY Y R  NPT +
Sbjct: 8   FETLQLHAGQEPDAATSSRAV-PIYATTSYVFRDCDHGGRLFGLQEPGYIYSRMMNPTAD 66

Query: 260 TLDR 271
             ++
Sbjct: 67  VFEK 70


>SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 390

 Score = 40.3 bits (90), Expect = 1e-04
 Identities = 20/52 (38%), Positives = 31/52 (59%)
 Frame = +2

Query: 110 TQAIHAGQDPDKWDSAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRNTL 265
           T+ +H   + D++ +++V  PI  S TFKQ        ++Y RSGNPTR+ L
Sbjct: 11  TELVHVEGNEDQYHASSV--PIYQSATFKQPCLEHMGKFDYTRSGNPTRSVL 60



 Score = 40.3 bits (90), Expect = 1e-04
 Identities = 18/47 (38%), Positives = 27/47 (57%)
 Frame = +3

Query: 276 LAALEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNRLL 416
           LA L   KHA V ++G+ A   I        H+++  D+YGG++RLL
Sbjct: 64  LAKLMKAKHAFVTSNGMSALDMILRCCKSNSHVVAGHDLYGGSDRLL 110


>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 767

 Score = 27.9 bits (59), Expect = 0.75
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = -2

Query: 424 TCLNNLFVPPYTSSED 377
           TC N L +PPYTS +D
Sbjct: 734 TCFNRLDLPPYTSKKD 749


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 26.6 bits (56), Expect = 1.7
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = -2

Query: 424 TCLNNLFVPPYTSSED 377
           TC N L +PPY S E+
Sbjct: 638 TCFNRLDIPPYNSKEE 653


>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
           Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 25.4 bits (53), Expect = 4.0
 Identities = 9/24 (37%), Positives = 14/24 (58%)
 Frame = +2

Query: 209 TVHNGYEYGRSGNPTRNTLDRMPR 280
           T HNG E+G +G P     + +P+
Sbjct: 629 TFHNGSEFGTTGVPDEPNEEELPK 652


>SPAC4F10.18 |||WD repeat protein, human NUP37
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 25.0 bits (52), Expect = 5.3
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +3

Query: 387 DVYGGTNRLLRQVVSRMGID 446
           DVY   NRL  QV++ +G D
Sbjct: 144 DVYSADNRLAEQVIASVGDD 163


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 24.6 bits (51), Expect = 7.0
 Identities = 12/39 (30%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
 Frame = +3

Query: 282 ALEGGKHALVFASGLGATTTIASLLSKGD-HILSSDDVY 395
           +++G    LV++   G+T  I  +   GD H+L   D+Y
Sbjct: 253 SIKGPGERLVYSMHSGSTLRIWEIFGTGDHHLLRGFDIY 291


>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 530

 Score = 24.6 bits (51), Expect = 7.0
 Identities = 11/38 (28%), Positives = 22/38 (57%)
 Frame = +2

Query: 152 SAAVVAPIVTSTTFKQTAPTVHNGYEYGRSGNPTRNTL 265
           S+AVV+ +V+S T    + T+ +      S +PT +++
Sbjct: 256 SSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSV 293


>SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 357

 Score = 24.2 bits (50), Expect = 9.3
 Identities = 13/42 (30%), Positives = 20/42 (47%)
 Frame = +3

Query: 285 LEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGGTNR 410
           +E GK+AL F +      T    +     ILS+  V+ G +R
Sbjct: 48  IESGKNALHFIASYAEKETEVQAIEVTKWILSNGGVWNGIDR 89


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 24.2 bits (50), Expect = 9.3
 Identities = 15/49 (30%), Positives = 24/49 (48%)
 Frame = +3

Query: 255 GTL*TECLAALEGGKHALVFASGLGATTTIASLLSKGDHILSSDDVYGG 401
           GT  ++ L  +E   H  VFA+G GA+    +   + D I +   + GG
Sbjct: 537 GTAKSQFLKYVEKTAHRAVFATGQGASAVGLTASVRKDPITNEWTLEGG 585


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,753,312
Number of Sequences: 5004
Number of extensions: 31801
Number of successful extensions: 95
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 164204010
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)